{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040000781994357888.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18H04167"},{"@type":"JGN","@value":"JP18H04167"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H04167/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"流動性足場・曲面足場設計に基づくオルガノイドの精密誘導技術の開発"},{"@language":"en","@value":"Development of precision induction of organoids based on biomaterial design of fluidic and curved extracellular matrix"}],"field":[{"keyword":[{"language":"ja","textList":["中区分90:人間医工学およびその関連分野"]},{"language":"en","textList":["Medium-sized Section 90:Biomedical engineering and related fields"]}]}],"dc:language":"ja","description":[{"type":"outline_of_research_achievement","notation":[{"@language":"ja","@value":"人工小臓器の芽構造であるオルガノイドを培養環境にて再現よく高効率に発生・誘導する技術と学理を開拓することを目的とし、オルガノイドの培養足場材料の粘弾性特性および形状誘導に係る曲面特性の各パラメーター効果を系統的に調べた。従来のオルガノイド作製で汎用されるマトリゲルのレオロジー特性を、追加架橋および機械的切断操作により調節しオルガノイド形成の最適動的粘弾性値を見出した。シンプルな合成高分子PEGにポリペプチドを修飾した物理架橋マトリックスを作製し、ラミニンを導入した系においてそれら粘弾性特性を設定することでマトリゲルの系よりも安定した肝オルガノイドの誘導に成功した。"},{"@language":"en","@value":"To develop a technique and basic understandings for high reproducible and efficient preparation of organoids, i.e., the budding structures of artificial small organs formed in a culture environment, we systematically investigated the effects of parameters of viscoelastic properties and shape-inducing curvature of scaffolds for organoid culture. The rheological properties of Matrigel, which is commonly used in conventional organoid culture, were modulated by additional cross-linking and mechanical fragmentation of the matrix to determine the optimal viscoelastic property of the matrix for organoid formation. A simple synthetic polymer, PEG, was physically crosslinked with modified polypeptides, and the determined optimal viscoelastic properties were introduced to a laminin-loaded system through regulating the strength of physical crosslinking. This synthetic polymer matrix successfully produced liver organoids more stable than in the Matrigel system."}]},{"type":"significance_of_research_achievement","notation":[{"@language":"ja","@value":"オルガノイド形成のための足場材料の力学物性と形状特性の影響に関する基礎研究は関連の分野で近年注目が集まっている。また実用的な観点でも、生物由来材料であるマトリゲルを合成高分子マトリックスに置き換えてオルガノイドを高効率かつ安定して誘導可能な足場材料の開発は強く望まれている状況にある。本研究ではオルガノイドの再現性の高い高効率誘導を可能とするマトリックスの粘弾性特性を特定し、合成高分子マトリックスにおいてその物性を導入することでマトリゲルの代替材料の学理と実際の材料構築に成功し、学術的および社会的意義の両面において本質的な成果を得ている。"}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1420282801193785984","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10336018"},{"@type":"NRID","@value":"1000010336018"},{"@type":"NRID","@value":"9000018555914"},{"@type":"NRID","@value":"9000412281495"},{"@type":"NRID","@value":"9000402585467"},{"@type":"NRID","@value":"9000006869316"},{"@type":"NRID","@value":"9000021468926"},{"@type":"NRID","@value":"9000003794580"},{"@type":"NRID","@value":"9000017713506"},{"@type":"NRID","@value":"9000023392726"},{"@type":"NRID","@value":"9000406357922"},{"@type":"NRID","@value":"9000255959990"},{"@type":"NRID","@value":"9000410177519"},{"@type":"NRID","@value":"9000331417138"},{"@type":"NRID","@value":"9000329413038"},{"@type":"NRID","@value":"9000308712667"},{"@type":"NRID","@value":"9000406375519"},{"@type":"NRID","@value":"9000017714157"},{"@type":"NRID","@value":"9000017712206"},{"@type":"NRID","@value":"9000329413324"},{"@type":"NRID","@value":"9000244012260"},{"@type":"NRID","@value":"9000000013447"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/satorukid_0101"}],"foaf:name":[{"@language":"ja","@value":"木戸秋 悟"},{"@language":"en","@value":"Kidoaki Satoru"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"}],"role":"principal_investigator"}],"since":"2018-04-01","until":"2022-03-31","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0017102"}],"notation":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific 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