{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040282256562277632.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"03556016"},{"@type":"JGN","@value":"JP03556016"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03556016/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"マツ材線虫病防除のための樹幹注入剤の開発"},{"@language":"en","@value":"Development of trunk injection agents for a pine-wilt desease"}],"field":[{"keyword":[{"language":"ja","textList":["農学","農芸化学","製造化学・食品"]},{"language":"en","textList":["Agriculture","Agricultural chemistry","製造化学・食品"]}]}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"ja","@value":"Dehydrosinefunginを大量生産するための生産条件を検討するために迅速にして正確なる定量法の確立が不可欠であった。イオン交換カラム処理によって10倍に濃縮した画分を試料としHPLCで本化合物の分離定量を行う系を確立した。本化合物とsinefungin,amidinosinefunginは塩基性のヌクレオシド類であるため一般のクロマトグラフィーでは分離が不十分であったが,ペアードイオンクロマトグラフィーを用いることにより分離可能となった。すなわち,ODSカラムを用い,1-ブタンスルホン酸ナトリウムをカウンターイオンとし,移動相に0.02Mリン酸一カリウム溶液を用いる条件が最も効果的であった。本法による定量値を指標にして,種々の培養条件の検討を行い,生産性を従来より10倍高めることに成功し,培養液1lあたり20mg以上生産できるようになった。この最適培養条件を用いて大量培養を行い,化合物の精製を試み,最終的におよそ200mgの精製標品を得た。この化合物量により,これまで行えなかった三年生松への投与実験が可能となった。\n一方,本化合物の松樹体への接種法についても,標品として,酒石酸モランテルを用いて,予備的な検討を加えた。三年生苗木の樹体に挿入したピペットチップから薬剤溶液を供試することにより,信頼できる結果が得られることがわかった。この方法でDehydrosinefunginを投与した三年生苗木にマツノザイセンチュウを後接種したところ,0.1mgの投与で防除効果が認められた。これは酒石酸モランテルの効果の10倍であり,本化合物が樹幹注入剤として有望であることが示された。今後さらに三年生苗木を用いる防除試験の再現性を確認するとともに,大径木を用いる防除試験を行う予定である。"},{"@language":"en","@value":"Dehydrosinefungin is the potent nematicidal compound, but the productivity of this compound by Strepromyces sp. H-114 is very low. Thus, an improvement of the productivity is needed. We developed the quantitative method for dehydrosinefungin to find the best conditions for production of this compound. Paired-ion chromatography using an HPLC aparatus resulted in\nthe best separation of dehydrosinefungin and its analogs, sinefungin and amidinosinefungin. Guided by the productivity of dehydrosinefungin, we determined the best culture conditions. Using the best conditions, the productivity reached more than 20 mg/l, that is, ten times as high as that of original conditions. Large scale cultivation using a jar-fermentor gave us more than 200 mg of pure dehydrosinefugin. This enabled us to carry out the assay using three-year pine trees.\nOn the other hand, we established the method for a trunk injection of the active compound using morantel tartarate as a test material. Using this method, dehydrosinefungin was injected to three-year pine trees before the treatment of nematodes. We found that 0.5 mg dehydrosinefungin per a three-year pine tree resulted in the resistance of tested trees against wilting. This result indicated that dehydrosinefungin was the promising agent for a pine-wilt desease."}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1030003658314228864","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30026520"},{"@type":"NRID","@value":"1000030026520"},{"@type":"NRID","@value":"9000002692992"},{"@type":"NRID","@value":"9000021163768"},{"@type":"NRID","@value":"9000020720333"},{"@type":"NRID","@value":"9000253151796"},{"@type":"NRID","@value":"9000253152098"},{"@type":"NRID","@value":"9000253084894"},{"@type":"NRID","@value":"9000253150566"},{"@type":"NRID","@value":"9000256469820"},{"@type":"NRID","@value":"9000003162582"},{"@type":"NRID","@value":"9000254651574"},{"@type":"NRID","@value":"9000252808220"},{"@type":"NRID","@value":"9000252804744"},{"@type":"NRID","@value":"9000253139249"},{"@type":"NRID","@value":"9000253142210"},{"@type":"NRID","@value":"9000020793322"},{"@type":"NRID","@value":"9000253147582"},{"@type":"NRID","@value":"9000254714653"},{"@type":"NRID","@value":"9000401541119"},{"@type":"NRID","@value":"9000002697193"},{"@type":"NRID","@value":"9000254195795"},{"@type":"NRID","@value":"9000254241818"},{"@type":"NRID","@value":"9000253144939"},{"@type":"NRID","@value":"9000021012216"},{"@type":"NRID","@value":"9000253148494"},{"@type":"NRID","@value":"9000253142823"},{"@type":"NRID","@value":"9000017624729"},{"@type":"NRID","@value":"9000020997943"},{"@type":"NRID","@value":"9000252805758"},{"@type":"NRID","@value":"9000002381107"},{"@type":"NRID","@value":"9000020819656"},{"@type":"NRID","@value":"9000253141726"},{"@type":"NRID","@value":"9000020770379"},{"@type":"NRID","@value":"9000020793475"},{"@type":"NRID","@value":"9000253152156"},{"@type":"NRID","@value":"9000401959465"},{"@type":"NRID","@value":"9000003142818"},{"@type":"NRID","@value":"9000252804785"},{"@type":"NRID","@value":"9000253142919"},{"@type":"NRID","@value":"9000020767996"},{"@type":"NRID","@value":"9000006768076"},{"@type":"NRID","@value":"9000020949906"},{"@type":"NRID","@value":"9000253141668"},{"@type":"NRID","@value":"9000254195545"},{"@type":"NRID","@value":"9000020846228"},{"@type":"NRID","@value":"9000021012194"},{"@type":"NRID","@value":"9000253138643"},{"@type":"NRID","@value":"9000256040886"},{"@type":"NRID","@value":"9000020717779"},{"@type":"NRID","@value":"9000255839080"},{"@type":"NRID","@value":"9000014349023"},{"@type":"NRID","@value":"9000021832164"},{"@type":"NRID","@value":"9000021901467"},{"@type":"NRID","@value":"9000253151474"},{"@type":"NRID","@value":"9000253085732"},{"@type":"NRID","@value":"9000253139246"},{"@type":"NRID","@value":"9000018398659"},{"@type":"NRID","@value":"9000004513958"}],"foaf:name":[{"@language":"ja","@value":"河津 一儀"},{"@language":"en","@value":"KAWAZU Kazuyoshi"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"岡山大学"},{"@language":"en","@value":"Okayama Univ., Fac. Agr. Prof"}],"role":"principal_investigator"},{"@id":"https://cir.nii.ac.jp/crid/1420564276175817600","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60183787"},{"@type":"NRID","@value":"1000060183787"},{"@type":"NRID","@value":"9000283849801"},{"@type":"NRID","@value":"9000252790404"},{"@type":"NRID","@value":"9000401541116"},{"@type":"NRID","@value":"9000021847717"},{"@type":"NRID","@value":"9000363292691"},{"@type":"NRID","@value":"9000006764503"},{"@type":"NRID","@value":"9000283255858"},{"@type":"NRID","@value":"9000363278082"},{"@type":"NRID","@value":"9000411567006"},{"@type":"NRID","@value":"9000257813416"},{"@type":"NRID","@value":"9000001836559"},{"@type":"NRID","@value":"9000257986781"},{"@type":"NRID","@value":"9000401959462"},{"@type":"NRID","@value":"9000343343991"},{"@type":"NRID","@value":"9000363292326"},{"@type":"NRID","@value":"9000363290751"},{"@type":"NRID","@value":"9000409027660"},{"@type":"NRID","@value":"9000020873934"},{"@type":"NRID","@value":"9000398151626"},{"@type":"NRID","@value":"9000257812984"},{"@type":"NRID","@value":"9000341540155"},{"@type":"NRID","@value":"9000021832168"},{"@type":"NRID","@value":"9000243853131"},{"@type":"NRID","@value":"9000406393618"},{"@type":"NRID","@value":"9000015777891"},{"@type":"NRID","@value":"9000363277245"},{"@type":"NRID","@value":"9000363276058"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0016182"}],"foaf:name":[{"@language":"ja","@value":"神崎 浩"},{"@language":"en","@value":"KANZAKI Hiroshi"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"岡山大学"},{"@language":"en","@value":"Okayama Univ. The graduate School Of Natural Science and Technology Assistant"}],"role":"co_investigator_buntan"},{"@id":"https://cir.nii.ac.jp/crid/1030003658361994497","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30115844"},{"@type":"NRID","@value":"1000030115844"},{"@type":"NRID","@value":"9000411038804"},{"@type":"NRID","@value":"9000411038823"}],"foaf:name":[{"@language":"ja","@value":"小林 昭雄"},{"@language":"en","@value":"KOBAYASHI Akio"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"岡山大学"},{"@language":"en","@value":"Okayama Univ. Agr. Prof."}],"role":"co_investigator_buntan"}],"since":"1991","until":"1992","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0015301"}],"notation":[{"@language":"ja","@value":"岡山大学"},{"@language":"en","@value":"Okayama University"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific Research"}]}],"url":[{"@id":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03556016/","notation":[{"@value":"KAKEN"}]}],"createdAt":"1991-03-31","modifiedAt":"2025-11-19","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%9E%E3%83%84%E3%83%8E%E3%82%B6%E3%82%A4%E3%82%BB%E3%83%B3%E3%83%81%E3%83%A5%E3%82%A6","dc:title":"マツノザイセンチュウ"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%AE%BA%E7%B7%9A%E8%99%AB%E7%89%A9%E8%B3%AA","dc:title":"殺線虫物質"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%A8%B9%E5%B9%B9%E6%B3%A8%E5%85%A5","dc:title":"樹幹注入"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%A0%B8%E9%85%B8%E7%B3%BB%E6%8A%97%E7%94%9F%E7%89%A9%E8%B3%AA","dc:title":"核酸系抗生物質"},{"@id":"https://cir.nii.ac.jp/all?q=dehydrosinefungin","dc:title":"dehydrosinefungin"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%9A%E3%82%A2-%E3%83%89%E3%82%A4%E3%82%AA%E3%83%B3%E3%82%AF%E3%83%AD%E3%83%9E%E3%83%88%E3%82%B0%E3%83%A9%E3%83%95%E3%82%A3-","dc:title":"ペア-ドイオンクロマトグラフィ-"},{"@id":"https://cir.nii.ac.jp/all?q=Nematicide","dc:title":"Nematicide"},{"@id":"https://cir.nii.ac.jp/all?q=Bursaphelenchus%20xylophilus","dc:title":"Bursaphelenchus xylophilus"},{"@id":"https://cir.nii.ac.jp/all?q=Paired-ion%20chromatography","dc:title":"Paired-ion chromatography"},{"@id":"https://cir.nii.ac.jp/all?q=Dehydrosinefungin","dc:title":"Dehydrosinefungin"},{"@id":"https://cir.nii.ac.jp/all?q=nucleoside%20antibiotic","dc:title":"nucleoside antibiotic"},{"@id":"https://cir.nii.ac.jp/all?q=trunk%20injection","dc:title":"trunk injection"}],"dataSourceIdentifier":[{"@type":"KAKEN","@value":"03556016"}],"grant":{"grantIdentifier":[{"@type":"NII","@value":"39"}],"jpcoar:fundingStream":[{"@language":"ja","@value":"試験研究(B)"},{"@language":"en","@value":"Grant-in-Aid for Developmental Scientific Research (B)"}]},"allocationClassification":[{"notation":[{"@language":"ja","@value":"補助金"},{"@language":"en","@value":"Single-year Grants"}]}],"projectStatus":"project_closed","allocationAmount":{"totalCost":{"amount":"8300000","currency":"JPY"},"breakdownCost":[{"notation":[{"@language":"ja","@value":"直接経費"},{"@language":"en","@value":"Direct Cost"}],"amount":"8300000","currency":"JPY"}]}}