{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040282256719065984.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"12460017"},{"@type":"JGN","@value":"JP12460017"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12460017/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"分子生物学的アプローチによる球根作物の球根形成および休眠に関する研究"},{"@language":"en","@value":"Studies on bulb formation and dormancy in bulbous plants by molecular biological approach"}],"field":[{"keyword":[{"language":"ja","textList":["農学","農学","園芸・造園学"]},{"language":"en","textList":["Agriculture","Agriculture","園芸・造園学"]}]}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"ja","@value":"本研究は,分子生物学的アプローチにより球根植物における休眠導入と球根形成開始との関係を明らかにすることを目的として行った.\nまず,in vitroにおける球根形成の制御法を確立することを試みた.ヒアシンス(Hyacinthus orientalis L.cv.Delft Blue)の球根内葉原基由来のシュート外植体を,8週間の5℃暗黒処理後,25℃連続照明下で培養すると,処理後4週間で球根を形成したが,無低温処理区では球根形成が抑制された.また,無低温であっても,ABA添加培地上では球根形成が促進され,逆に,低温処理下であっても,フルリドンを培地に添加すると球根形成は抑制された.さらに低温処理区においてのみ,内生ABAが増加した.\n次に,低温およびABA処理によって誘導される球根形成遺伝子の探索をディファレンシャルディスプレイ法により行った.低温およびABA処理を行った外植体にPCR増幅断片の多型がみられた.また,低温処理により誘導された特異的なPCR増幅断片のうち,6断片がABA処理区でも特異的に発現し,2断片の発現が両処理区で共通して抑制されていた.\nそこで,低温処理により誘導された18遺伝子断片の塩基配列を決定するとともに,得られた遺伝子断片と既知遺伝子との相同性検索を行った.これらの遺伝子断片のうち8断片は17種類の既知遺伝子と80%以上の相同性を示した、低温およびABAにより共通して誘導された遺伝子断片は,Arabidopsis thalianaやOriza sativaのゲノムDNAと相同性を示した.また,低温誘導性の3断片は,サツマイモのデンプン合成に関わる遺伝子の配列と部分的な一致を示した.既知遺伝子との相同性が低かった10断片は新規な遺伝子である可能性が考えられた.\n以上のことから,球根植物における休眠導入と球根形成開始とは同一の現象であるという考え方を分子レベルにおいても支持していると考えられる."},{"@language":"en","@value":"Molecular biological studies were used to investigate the hypothesis that the induction of bulb formation and the induction of bulb dormancy are the same phenomenon. Shoot explants were taken from in vitro immature leaves of hyacinth (Hyacinthus orientalis L. cv. Delft Blue) and cultured at 25℃ on MS medium containing NAA and BA. The explants formed bulbs four after the cold treatment at 5℃, whereas bulb formation was supressed without cold treatment. ABA promoted bulb formation without cold treatment. By flundone, bulb formation of the cold treated explants was inhibited. Under bulb-forming conditions, endogenous ABA content increased. These results suggest that bulb formation is induced by low temperature and regulated by ABA. Thus, the controlling methods are bulb forming conditions are obtainable by cold treatment or BAB without cold treatment and non-bulb forming conditions are by non-cold treatment or fluridone with cold treatment.\nDifferential display analysis of genes induced by low temperature and ABA in bulb formation of hyacinth was conducted. Differential PCR amplified fragments between cold-treated and non-cold-treated shoot samples were observed. Six differential PCR amplified fragments were commonly expressed both in the cold and ABA treatments, and two were suppressed in both treatments. The cDNA amplified fragments observed in both treatments might be related to bulb formation in hyacinth.\nThe partial sequences of the cDNA clones obtained from 18 specific gene fragments by low temperature (designated as HL clones) were carried out. Eight HL clones had over 80% identity with identified sequences with FASTA program. The HL clones of cDNA induced by low temperature and ABA were homologous to genome DNA of A. thaliana or O. sativa. Three HL clones showed partial identity with starch phosphorylase gene of I. batatas. Some HL clones were homologous to genes or mRNAs of which the function had been suggested. It is possible that HL clones of which the functions were not clarified are novel genes related to bulb formation."}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1030003658364633860","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80150506"},{"@type":"NRID","@value":"1000080150506"},{"@type":"NRID","@value":"9000002226017"},{"@type":"NRID","@value":"9000364797182"},{"@type":"NRID","@value":"9000021756590"},{"@type":"NRID","@value":"9000002225855"},{"@type":"NRID","@value":"9000413871730"},{"@type":"NRID","@value":"9000021110194"},{"@type":"NRID","@value":"9000002225614"},{"@type":"NRID","@value":"9000021755055"},{"@type":"NRID","@value":"9000007740425"},{"@type":"NRID","@value":"9000021107487"},{"@type":"NRID","@value":"9000002226414"},{"@type":"NRID","@value":"9000002226416"},{"@type":"NRID","@value":"9000002506682"},{"@type":"NRID","@value":"9000002225971"},{"@type":"NRID","@value":"9000364959065"},{"@type":"NRID","@value":"9000363158355"},{"@type":"NRID","@value":"9000363158534"},{"@type":"NRID","@value":"9000363159076"},{"@type":"NRID","@value":"9000363158827"},{"@type":"NRID","@value":"9000363158697"},{"@type":"NRID","@value":"9000363158458"},{"@type":"NRID","@value":"9000363158671"},{"@type":"NRID","@value":"9000363158341"},{"@type":"NRID","@value":"9000363158554"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0067472"}],"foaf:name":[{"@language":"ja","@value":"大久保 敬"},{"@language":"en","@value":"OKUBO Hiroshi"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Professor"}],"role":"principal_investigator"},{"@id":"https://cir.nii.ac.jp/crid/1420845751138232704","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30264104"},{"@type":"NRID","@value":"1000030264104"},{"@type":"NRID","@value":"9000002226337"},{"@type":"NRID","@value":"9000021102078"},{"@type":"NRID","@value":"9000006548010"},{"@type":"NRID","@value":"9000002227401"},{"@type":"NRID","@value":"9000241580248"},{"@type":"NRID","@value":"9000024955713"},{"@type":"NRID","@value":"9000005831298"},{"@type":"NRID","@value":"9000260189668"},{"@type":"NRID","@value":"9000283621838"},{"@type":"NRID","@value":"9000018879295"},{"@type":"NRID","@value":"9000018931689"},{"@type":"NRID","@value":"9000258082026"},{"@type":"NRID","@value":"9000003137982"},{"@type":"NRID","@value":"9000381493918"},{"@type":"NRID","@value":"9000258081880"},{"@type":"NRID","@value":"9000014187428"},{"@type":"NRID","@value":"9000258081318"},{"@type":"NRID","@value":"9000258081747"},{"@type":"NRID","@value":"9000002227165"},{"@type":"NRID","@value":"9000003087053"},{"@type":"NRID","@value":"9000408435686"},{"@type":"NRID","@value":"9000258081828"},{"@type":"NRID","@value":"9000018130873"},{"@type":"NRID","@value":"9000344800225"},{"@type":"NRID","@value":"9000410647042"},{"@type":"NRID","@value":"9000411496873"},{"@type":"NRID","@value":"9000260199266"},{"@type":"NRID","@value":"9000283621961"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/hiramatsu_michikazu"}],"foaf:name":[{"@language":"ja","@value":"比良松 道一"},{"@language":"en","@value":"HIRAMATSU Michikazu"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Assistant Professor"}],"role":"co_investigator_buntan"},{"@id":"https://cir.nii.ac.jp/crid/1420001326233579776","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60253514"},{"@type":"NRID","@value":"1000060253514"},{"@type":"ORCID","@value":"0000-0003-3005-5451"},{"@type":"NRID","@value":"9000411497988"},{"@type":"NRID","@value":"9000002227113"},{"@type":"NRID","@value":"9000413871751"},{"@type":"NRID","@value":"9000342446483"},{"@type":"NRID","@value":"9000413871746"},{"@type":"NRID","@value":"9000414834628"},{"@type":"NRID","@value":"9000260190009"},{"@type":"NRID","@value":"9000408574080"},{"@type":"NRID","@value":"9000408888152"},{"@type":"NRID","@value":"9000006855114"},{"@type":"NRID","@value":"9000006339681"},{"@type":"NRID","@value":"9000413871758"},{"@type":"NRID","@value":"9000408574084"},{"@type":"NRID","@value":"9000005831473"},{"@type":"NRID","@value":"9000260189978"},{"@type":"NRID","@value":"9000402454485"},{"@type":"NRID","@value":"9000415125500"},{"@type":"NRID","@value":"9000005831289"},{"@type":"NRID","@value":"9000406046692"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/hortsci4564"}],"foaf:name":[{"@language":"ja","@value":"尾崎 行生"},{"@language":"en","@value":"OZAKI Yukio"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Assistant Professor"}],"role":"co_investigator_buntan"},{"@id":"https://cir.nii.ac.jp/crid/1030003658330955521","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20182024"},{"@type":"NRID","@value":"1000020182024"},{"@type":"NRID","@value":"9000001989082"},{"@type":"NRID","@value":"9000412188250"},{"@type":"NRID","@value":"9000023608194"},{"@type":"NRID","@value":"9000312729103"},{"@type":"NRID","@value":"9000405731358"},{"@type":"NRID","@value":"9000003137977"},{"@type":"NRID","@value":"9000329020607"},{"@type":"NRID","@value":"9000258082197"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0118239"}],"foaf:name":[{"@language":"ja","@value":"宮島 郁夫"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"}],"role":"co_investigator_buntan"}],"since":"2000","until":"2002","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0017102"}],"notation":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific Research"}]}],"url":[{"@id":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12460017/","notation":[{"@value":"KAKEN"}]}],"createdAt":"2000-03-31","modifiedAt":"2025-11-20","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E7%90%83%E6%A0%B9","dc:title":"球根"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%BC%91%E7%9C%A0","dc:title":"休眠"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%90%83%E6%A0%B9%E5%BD%A2%E6%88%90","dc:title":"球根形成"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%A2%E3%83%96%E3%82%B7%E3%82%B8%E3%83%B3%E9%85%B8","dc:title":"アブシジン酸"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%90%83%E5%BD%A2%E6%88%90","dc:title":"球形成"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%87%E3%82%A3%E3%83%95%E3%82%A1%E3%83%AC%E3%83%B3%E3%82%B7%E3%83%A3%E3%83%AB%E3%83%82%E3%82%B9%E3%83%97%E3%83%AC%E3%82%A4","dc:title":"ディファレンシャルヂスプレイ"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%A2%E3%83%97%E3%82%B7%E3%82%B8%E3%83%B3%E9%85%B8","dc:title":"アプシジン酸"},{"@id":"https://cir.nii.ac.jp/all?q=Bulbs","dc:title":"Bulbs"},{"@id":"https://cir.nii.ac.jp/all?q=Dormancy","dc:title":"Dormancy"},{"@id":"https://cir.nii.ac.jp/all?q=Bulb%20initiation","dc:title":"Bulb initiation"},{"@id":"https://cir.nii.ac.jp/all?q=Abscisic%20acid","dc:title":"Abscisic acid"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"}],"dataSourceIdentifier":[{"@type":"KAKEN","@value":"12460017"}],"grant":{"grantIdentifier":[{"@type":"NII","@value":"59"}],"jpcoar:fundingStream":[{"@language":"ja","@value":"基盤研究(B)"},{"@language":"en","@value":"Grant-in-Aid for Scientific Research (B)"}]},"allocationClassification":[{"notation":[{"@language":"ja","@value":"補助金"},{"@language":"en","@value":"Single-year Grants"}]}],"projectStatus":"project_closed","allocationAmount":{"totalCost":{"amount":"13900000","currency":"JPY"},"breakdownCost":[{"notation":[{"@language":"ja","@value":"直接経費"},{"@language":"en","@value":"Direct Cost"}],"amount":"13900000","currency":"JPY"}]}}