{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040282256783022976.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15380026"},{"@type":"JGN","@value":"JP15380026"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380026/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"香酸カンキツにおける細胞質-核雄性不稔に関する研究"},{"@language":"en","@value":"Studies on the cytoplasmic-genic male sterility in acid citrus"}],"field":[{"keyword":[{"language":"ja","textList":["生物系","農学","農学","園芸学・造園学"]},{"language":"en","textList":["Biological Sciences","Agriculture","Agriculture","Horticulture/Landscape architecture"]}]}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"ja","@value":"本研究は各種酢ミカン類の多数の交配組合せから得た10年生雑種実生群を主に用いて雄性不稔の遺伝解明とRAPDマーカーによる雄性不稔雑種の早期選抜に力点を置いたものである。この3年間に行ったこれらの研究とその成果の概要は以下の通りである。\n雄性不稔は、1)独立の3遺伝子ms_1 ms_2 ms_3に支配され、劣性ホモで発現すること、2)3遺伝子間に相互作用があり、ms_1とms_2は補足遺伝子、ms_3は優性上位の遺伝子であること、3)驚くべきことに、交配に用いたほとんど(12品種)の遺伝子型はヘテロであること、4)スダチの細胞質には稔性回復因子R^+があること、が分った。ウンシュウミカンの細胞質と関連して見られる葯退化は酢ミカン類の雄性不稔雑種群には出現しなかった。\nこれらの雑種個体の中で、ユズ、ハナユ、カボスおよびスダチの自家および他家交雑実生に、低頻度(約1%)ではあるが、幼樹開花実生が出現した。さらに、ユズの戻し交配やグレープフルーツとの交配で高頻度(それぞれ6%と16%)に幼樹開花性雑種実生群を分離する単胚性雄性不稔雑種HY-16(ハナユ×ユズ)を発見し、期待通りに稔性:不稔性がそれぞれ5:3と1:1に分離することを証明した。\n以上の結果を基に、主要酢ミカン12種類の雄性不稔遺伝子型を決定した。雄性不稔雑種実生と雄性稔性雑種実生をそれぞれ10個体ずつバルクとして、約100のランダムプライマーを用いて、RAPD分析を進めた結果、HY16×フォスターピンクグレープフルーツのF1雑種実生群で雄性不稔と密接に関連(組換価6.3%)するRAPDマーカー(プライマーCMN-A10)を見出した。\n雄性不稔葯の発達過程の細胞・組織学的調査の結果、胞原期に退化が始まる個体と減数分裂期前後にタペート細胞の退化が始まる個体があり、前者は小型の葯、後者は中型の葯となること、小型の不稔葯形成はms_2、中型の不稔葯形成はms_1が支配することが示唆された。"},{"@language":"en","@value":"This study was focused on the certification of genetic control of male sterility in ten-year-old acid citrus seedlings derived from various cross combinations and the early selection of male sterile hybrid seedlings with RAPD markers. The results of study carried out for three years were as follows.\nIt was found that (1) the male sterility was controlled by independent three genes ms1, ms2 and ms3 that are recessive, (2)additive gene effect was recognized among three genes : ms1 and ms2 are complementary genes and ms3 is a dominant gene with epistasis effect for ms1 and ms2. (3)Amazingly, genotypes of citrus cultivars used in the present study were heterozygous for the ms genes. (4)Cytoplasm of Sudachi had a factor to restore the male sterility in the progenies.\nPrecocious flowering in 3- to 5-month-old hybrid seedlings from crosses with Yuzu, Hanayu, Kabosu and Sudachi was observed. Monoembryonic and male sterile HY-16, which was derived from Hanayu x Yuzu, was produced precocious hybrid seedlings with high frequencies (6% for HY16 x Yuzu and 16% for HY16 x Foster Pink grapefruit). In these seedlings, male sterile and fertile seedlings segregated 5 : 3 for HY16 x Yuzu and 1: 1 for HY16 x Foster Pink grapefruit, suggesting the estimated male sterility genotypes for HY16 and Yuzu were correct.\nBased on these results, genotypes of 12 citrus cultivars were determined. Furthermore, RAPD markers linked to ms genes were studies with a bulk method and analyzed with about 100 random primers. One RAPD marker as to CMN-A10 primer was found to link to ms1 gene with a distance of 6.3 cM. The cytological observation of anther development in male sterile seedlings indicated that microsporogenesis were inhibited before and after meiosis."}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1030003658323495936","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10158579"},{"@type":"NRID","@value":"1000010158579"},{"@type":"NRID","@value":"9000002226335"},{"@type":"NRID","@value":"9000241761583"},{"@type":"NRID","@value":"9000270285252"},{"@type":"NRID","@value":"9000260189666"},{"@type":"NRID","@value":"9000003077141"},{"@type":"NRID","@value":"9000318141299"},{"@type":"NRID","@value":"9000017855411"},{"@type":"NRID","@value":"9000018500307"},{"@type":"NRID","@value":"9000410647922"},{"@type":"NRID","@value":"9000402211478"},{"@type":"NRID","@value":"9000283621791"},{"@type":"NRID","@value":"9000002227164"},{"@type":"NRID","@value":"9000341529763"},{"@type":"NRID","@value":"9000405228421"},{"@type":"NRID","@value":"9000258082051"},{"@type":"NRID","@value":"9000003083373"},{"@type":"NRID","@value":"9000003087086"},{"@type":"NRID","@value":"9000006208184"},{"@type":"NRID","@value":"9000020272523"},{"@type":"NRID","@value":"9000258081709"},{"@type":"NRID","@value":"9000003621999"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0172336"}],"foaf:name":[{"@language":"ja","@value":"若菜 章"},{"@language":"en","@value":"WAKANA Akira"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Plant Resources, Associate Professor"}],"role":"principal_investigator"},{"@id":"https://cir.nii.ac.jp/crid/1420001326233579776","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60253514"},{"@type":"NRID","@value":"1000060253514"},{"@type":"ORCID","@value":"0000-0003-3005-5451"},{"@type":"NRID","@value":"9000411497988"},{"@type":"NRID","@value":"9000002227113"},{"@type":"NRID","@value":"9000413871751"},{"@type":"NRID","@value":"9000342446483"},{"@type":"NRID","@value":"9000413871746"},{"@type":"NRID","@value":"9000414834628"},{"@type":"NRID","@value":"9000260190009"},{"@type":"NRID","@value":"9000408574080"},{"@type":"NRID","@value":"9000408888152"},{"@type":"NRID","@value":"9000006855114"},{"@type":"NRID","@value":"9000006339681"},{"@type":"NRID","@value":"9000413871758"},{"@type":"NRID","@value":"9000408574084"},{"@type":"NRID","@value":"9000005831473"},{"@type":"NRID","@value":"9000260189978"},{"@type":"NRID","@value":"9000402454485"},{"@type":"NRID","@value":"9000415125500"},{"@type":"NRID","@value":"9000005831289"},{"@type":"NRID","@value":"9000406046692"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/hortsci4564"}],"foaf:name":[{"@language":"ja","@value":"尾崎 行生"},{"@language":"en","@value":"OZAKI Yukio"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Plant Resources, Assistant Professor"}],"role":"co_investigator_buntan"}],"since":"2003","until":"2005","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0017102"}],"notation":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"KYUSHU UNIVERSITY"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific Research"}]}],"url":[{"@id":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380026/","notation":[{"@value":"KAKEN"}]}],"createdAt":"2003-03-31","modifiedAt":"2025-11-20","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%AB%E3%83%B3%E3%82%AD%E3%83%84","dc:title":"カンキツ"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%B4%B0%E8%83%9E%E8%B3%AA-%E6%A0%B8%E9%9B%84%E6%80%A7%E4%B8%8D%E7%A8%94","dc:title":"細胞質-核雄性不稔"},{"@id":"https://cir.nii.ac.jp/all?q=%E8%A3%9C%E8%B6%B3%E9%81%BA%E4%BC%9D%E5%AD%90","dc:title":"補足遺伝子"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%B8%8A%E4%BD%8D%E9%81%BA%E4%BC%9D%E5%AD%90","dc:title":"上位遺伝子"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%B4%B0%E8%83%9E%E8%B3%AA%E7%A8%94%E6%80%A7%E5%9B%9E%E5%BE%A9%E5%9B%A0%E5%AD%90","dc:title":"細胞質稔性回復因子"},{"@id":"https://cir.nii.ac.jp/all?q=RAPD%E3%83%9E%E3%83%BC%E3%82%AB%E3%83%BC","dc:title":"RAPDマーカー"},{"@id":"https://cir.nii.ac.jp/all?q=%E5%B9%BC%E6%A8%B9%E9%96%8B%E8%8A%B1","dc:title":"幼樹開花"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%85%A2%E3%83%9F%E3%82%AB%E3%83%B3","dc:title":"酢ミカン"},{"@id":"https://cir.nii.ac.jp/all?q=%E5%84%AA%E6%80%A7%E4%B8%8A%E4%BD%8D%E9%81%BA%E4%BC%9D%E5%AD%90","dc:title":"優性上位遺伝子"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%A6%99%E9%85%B8%E3%82%AB%E3%83%B3%E3%82%AD%E3%83%84","dc:title":"香酸カンキツ"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%84%A1%E6%A0%B8","dc:title":"無核"},{"@id":"https://cir.nii.ac.jp/all?q=PAPD","dc:title":"PAPD"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%80%A3%E9%8E%96","dc:title":"連鎖"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%9B%8C%E6%80%A7%E4%B8%8D%E7%A8%94","dc:title":"雌性不稔"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%9B%84%E6%80%A7%E4%B8%8D%E7%A8%94","dc:title":"雄性不稔"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%BF%E3%83%9A%E3%83%BC%E3%83%88%E7%B4%B0%E8%83%9E","dc:title":"タペート細胞"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%A0%B8-%E7%B4%B0%E8%83%9E%E8%B3%AA%E9%81%BA%E4%BC%9D","dc:title":"核-細胞質遺伝"},{"@id":"https://cir.nii.ac.jp/all?q=%E5%88%86%E5%AD%90%E3%83%9E%E3%83%BC%E3%82%AB%E3%83%BC","dc:title":"分子マーカー"},{"@id":"https://cir.nii.ac.jp/all?q=Citrus","dc:title":"Citrus"},{"@id":"https://cir.nii.ac.jp/all?q=Cytoplasmic-genic%20male%20sterility","dc:title":"Cytoplasmic-genic male sterility"},{"@id":"https://cir.nii.ac.jp/all?q=Complementary%20gene","dc:title":"Complementary gene"},{"@id":"https://cir.nii.ac.jp/all?q=Epistasis%20of%20gene","dc:title":"Epistasis of gene"},{"@id":"https://cir.nii.ac.jp/all?q=Cytoplasmic%20factor%20for%20Restoration%20of%20male%20sterility","dc:title":"Cytoplasmic factor for Restoration of male sterility"},{"@id":"https://cir.nii.ac.jp/all?q=RAPD%20marker","dc:title":"RAPD marker"},{"@id":"https://cir.nii.ac.jp/all?q=Precocious%20flowering","dc:title":"Precocious flowering"},{"@id":"https://cir.nii.ac.jp/all?q=Acid%20citrus","dc:title":"Acid citrus"}],"product":[{"@id":"https://cir.nii.ac.jp/crid/1010282256783022976","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Frequent occurrence of precocious flowering in zygotic seedlings derived from cross with a monoembryonic and male sterile acid citrus hybrid between Yuzu(C.junos Sieb, ex Tanaka) and Hanayu(C.hanaju Hort, ex Shirai)"}]},{"@id":"https://cir.nii.ac.jp/crid/1010282256783022977","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with colchicines"}]},{"@id":"https://cir.nii.ac.jp/crid/1010282256783022978","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Frequent occurrence of precocious flowering in zygotic seedlings derived from crosses with a monocembryonic and male sterile acid citrus hybrid between Yuzu (C.junos Sieb, ex Tanaka) and Hanayu (C.hanaju Hort.ex Shirai)."}]},{"@id":"https://cir.nii.ac.jp/crid/1010282256783022980","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Frequent occurrence of precocious flowering in zygotic seedlings derived from crosses with a monoembryonic and male sterile acid citrus hybrid between Yuzu (C.junos Sieb.ex Tanaka) and Hanayu (C.hanaju Hort.ex Shirai)"}]},{"@id":"https://cir.nii.ac.jp/crid/1010282256783022981","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with cilchicine."}]}],"dataSourceIdentifier":[{"@type":"KAKEN","@value":"15380026"}],"grant":{"grantIdentifier":[{"@type":"NII","@value":"69"}],"jpcoar:fundingStream":[{"@language":"ja","@value":"基盤研究(B)"},{"@language":"en","@value":"Grant-in-Aid for Scientific Research (B)"}]},"allocationClassification":[{"notation":[{"@language":"ja","@value":"補助金"},{"@language":"en","@value":"Single-year Grants"}]}],"projectStatus":"project_closed","allocationAmount":{"totalCost":{"amount":"11200000","currency":"JPY"},"breakdownCost":[{"notation":[{"@language":"ja","@value":"直接経費"},{"@language":"en","@value":"Direct Cost"}],"amount":"11200000","currency":"JPY"}]}}