{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040282256783025408.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15380027"},{"@type":"JGN","@value":"JP15380027"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380027/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"球根作物の球根形成を誘導する遺伝子の単離・同定"},{"@language":"en","@value":"Isolation and identification of the genes inducing bulb formation in bulbous plants"}],"field":[{"keyword":[{"language":"ja","textList":["生物系","農学","農学","園芸学・造園学"]},{"language":"en","textList":["Biological Sciences","Agriculture","Agriculture","Horticulture/Landscape architecture"]}]}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"ja","@value":"ヒアシンスおおびカノコユリにおける球根形成および球根被形成条件を確立した.\nディファレンシャルディスプレイによりヒアシンスとカノコユリの両方において,球根形成条件下で共通して発現した特異的断片が8本,共通して発現が抑制された断片が9本得られた.両種で共通して発現した特異的遺伝子断片のうち,OPA-08のプライマーで増幅された1000bpの断片はヒアシンスの5℃処理区と25℃+ABA処理区で,OPA-19で増幅された500bpの断片はヒアシンスの5℃処理区とカノコユリの25℃+ABA処理区で塩基配列の相同性が98%以上と非常に高かったことから,これらは球根形成制御に関与する遺伝子断片である可能性が考えられる.\nABA合成を経由しない低温のシグナル経路と,ABAのシグナル経路間のクロストークにおける中心的な存在であり,それぞれのストレス誘導性遺伝子の発現を調節しているCIPK3(タンパク質リン酸化酵素)遺伝子の単離をdegenerate PCRにより,カノコユリにおいて試みた.PCRにより,675bpのCIPK3 cDNA断片が得られ,degenerateプライマー作成に用いた6種の植物のCIPK3との相同性は65-71%であった.\n球根形成種アサツキと非形成種チャイブとの交配で昨年度得られたF_1個体にアサツキを戻し交配して,BC_1作出を行った.次に,得られたBC_1種子を,ホルモンフリーのMS培地に無菌播種し、発芽した実生を、25℃恒温・8時間日長下で1ヶ月間培養後、16時間日長下で60日間栽培した。その結果,BC_1の球根比の値は,非球根形成種チャイブに近い値から球根形成種アサツキに近い値まで広く分布した.球根比を元にBC_1個体群を球根形成集団と非球根形成集団の2Bulk集団に分類し,100種類のランダムプライマーを用いてRAPD分析を行った.その結果,各Bulk集団にのみ特異的に出現するマーカーは得られなかったが,いくつかのマーカーでは,球根形成個体に多く出現するバンドが検出された."},{"@language":"en","@value":"Conditions for controlling bulb formation and non-bulb formation in hyacinth (Hyacinthus orientalis) and lily (Lilium speciosum) were established. Under the bulb forming conditions, eight specific fragments were expressed and nine were suppressed in both species. The DNA sequence of the two fragments, one fragment of 1,000 bp amplified with a primer OPA-08 found in 5C treated and in 25C+ABA treated hyacinth, and another fragment of 500bp amplified with a primer OPA-19 found in 25C+ABA treated lily, showed 98% homology These are considered to be the possible genes that control bulb formation.\nIsolation of CIPK3 gene in lily was conducted by degenerative PCR. A fragment of CIPK3 cDNA of 675bp was obtained and it shouwed 65-71% homology with those of six species used for the preparation of degenerate primers.\nTwo types of Allium scjoenoprasum, one is genetically bulb forming type and the other is genetically non-bulb forming type were used for crossing to obtain F_1 and BC_1 plants. In vitro culture system was established for early identification of bulb formation of the plants. RAPD analysis using 100 random primers was applied to the bulked plants, one for bulb forming group and the other for non bulb forming group that were separated in BC_1 progenies. No specific markers were obtained."}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1420001326217062272","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10333759"},{"@type":"NRID","@value":"1000010333759"},{"@type":"NRID","@value":"9000002227490"},{"@type":"NRID","@value":"9000018154381"},{"@type":"NRID","@value":"9000006623956"},{"@type":"NRID","@value":"9000023608167"},{"@type":"NRID","@value":"9000404789706"},{"@type":"NRID","@value":"9000006624119"},{"@type":"NRID","@value":"9000257946459"},{"@type":"NRID","@value":"9000003078761"},{"@type":"NRID","@value":"9000283621329"},{"@type":"NRID","@value":"9000015330134"},{"@type":"NRID","@value":"9000413918395"},{"@type":"NRID","@value":"9000258081588"},{"@type":"NRID","@value":"9000283117553"},{"@type":"NRID","@value":"9000309184908"},{"@type":"NRID","@value":"9000258081526"},{"@type":"NRID","@value":"9000283621266"},{"@type":"NRID","@value":"9000260189976"},{"@type":"NRID","@value":"9000287294486"},{"@type":"NRID","@value":"9000260201775"},{"@type":"NRID","@value":"9000347662756"},{"@type":"NRID","@value":"9000329020605"},{"@type":"NRID","@value":"9000412652869"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0072338"}],"foaf:name":[{"@language":"ja","@value":"嬉野 健次"},{"@language":"en","@value":"URESHINO Kenji"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"岩手大学"},{"@language":"en","@value":"Iwate University, Faculty of Agriculture, Lecturer"}],"role":"co_investigator_buntan"},{"@id":"https://cir.nii.ac.jp/crid/1030003658364633860","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80150506"},{"@type":"NRID","@value":"1000080150506"},{"@type":"NRID","@value":"9000002226017"},{"@type":"NRID","@value":"9000364797182"},{"@type":"NRID","@value":"9000021756590"},{"@type":"NRID","@value":"9000002225855"},{"@type":"NRID","@value":"9000413871730"},{"@type":"NRID","@value":"9000021110194"},{"@type":"NRID","@value":"9000002225614"},{"@type":"NRID","@value":"9000021755055"},{"@type":"NRID","@value":"9000007740425"},{"@type":"NRID","@value":"9000021107487"},{"@type":"NRID","@value":"9000002226414"},{"@type":"NRID","@value":"9000002226416"},{"@type":"NRID","@value":"9000002506682"},{"@type":"NRID","@value":"9000002225971"},{"@type":"NRID","@value":"9000364959065"},{"@type":"NRID","@value":"9000363158355"},{"@type":"NRID","@value":"9000363158534"},{"@type":"NRID","@value":"9000363159076"},{"@type":"NRID","@value":"9000363158827"},{"@type":"NRID","@value":"9000363158697"},{"@type":"NRID","@value":"9000363158458"},{"@type":"NRID","@value":"9000363158671"},{"@type":"NRID","@value":"9000363158341"},{"@type":"NRID","@value":"9000363158554"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0067472"}],"foaf:name":[{"@language":"ja","@value":"大久保 敬"},{"@language":"en","@value":"OKUBO Hiroshi"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Professor"}],"role":"principal_investigator"},{"@id":"https://cir.nii.ac.jp/crid/1420845751138232704","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30264104"},{"@type":"NRID","@value":"1000030264104"},{"@type":"NRID","@value":"9000002226337"},{"@type":"NRID","@value":"9000021102078"},{"@type":"NRID","@value":"9000006548010"},{"@type":"NRID","@value":"9000002227401"},{"@type":"NRID","@value":"9000241580248"},{"@type":"NRID","@value":"9000024955713"},{"@type":"NRID","@value":"9000005831298"},{"@type":"NRID","@value":"9000260189668"},{"@type":"NRID","@value":"9000283621838"},{"@type":"NRID","@value":"9000018879295"},{"@type":"NRID","@value":"9000018931689"},{"@type":"NRID","@value":"9000258082026"},{"@type":"NRID","@value":"9000003137982"},{"@type":"NRID","@value":"9000381493918"},{"@type":"NRID","@value":"9000258081880"},{"@type":"NRID","@value":"9000014187428"},{"@type":"NRID","@value":"9000258081318"},{"@type":"NRID","@value":"9000258081747"},{"@type":"NRID","@value":"9000002227165"},{"@type":"NRID","@value":"9000003087053"},{"@type":"NRID","@value":"9000408435686"},{"@type":"NRID","@value":"9000258081828"},{"@type":"NRID","@value":"9000018130873"},{"@type":"NRID","@value":"9000344800225"},{"@type":"NRID","@value":"9000410647042"},{"@type":"NRID","@value":"9000411496873"},{"@type":"NRID","@value":"9000260199266"},{"@type":"NRID","@value":"9000283621961"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/hiramatsu_michikazu"}],"foaf:name":[{"@language":"ja","@value":"比良松 道一"},{"@language":"en","@value":"HIRAMATSU Michikazu"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Assistant Professor"}],"role":"co_investigator_buntan"},{"@id":"https://cir.nii.ac.jp/crid/1030003658501981696","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90110650"},{"@type":"NRID","@value":"1000090110650"},{"@type":"NRID","@value":"9000004457631"},{"@type":"NRID","@value":"9000021109791"},{"@type":"NRID","@value":"9000021106664"},{"@type":"NRID","@value":"9000021079729"},{"@type":"NRID","@value":"9000021996971"},{"@type":"NRID","@value":"9000021079009"},{"@type":"NRID","@value":"9000021756865"},{"@type":"NRID","@value":"9000022007786"},{"@type":"NRID","@value":"9000021109779"}],"foaf:name":[{"@language":"ja","@value":"稲田 委久子"},{"@language":"en","@value":"INADA Ikuko"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"岩手大学"},{"@language":"en","@value":"Iwate University, Faculty of Agriculture, Associate Professor"}],"role":"co_investigator_buntan"},{"@id":"https://cir.nii.ac.jp/crid/1420001326233579776","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60253514"},{"@type":"NRID","@value":"1000060253514"},{"@type":"ORCID","@value":"0000-0003-3005-5451"},{"@type":"NRID","@value":"9000411497988"},{"@type":"NRID","@value":"9000002227113"},{"@type":"NRID","@value":"9000413871751"},{"@type":"NRID","@value":"9000342446483"},{"@type":"NRID","@value":"9000413871746"},{"@type":"NRID","@value":"9000414834628"},{"@type":"NRID","@value":"9000260190009"},{"@type":"NRID","@value":"9000408574080"},{"@type":"NRID","@value":"9000408888152"},{"@type":"NRID","@value":"9000006855114"},{"@type":"NRID","@value":"9000006339681"},{"@type":"NRID","@value":"9000413871758"},{"@type":"NRID","@value":"9000408574084"},{"@type":"NRID","@value":"9000005831473"},{"@type":"NRID","@value":"9000260189978"},{"@type":"NRID","@value":"9000402454485"},{"@type":"NRID","@value":"9000415125500"},{"@type":"NRID","@value":"9000005831289"},{"@type":"NRID","@value":"9000406046692"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/hortsci4564"}],"foaf:name":[{"@language":"ja","@value":"尾崎 行生"},{"@language":"en","@value":"OZAKI Yukio"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University, Faculty of Agriculture, Assistant Professor"}],"role":"co_investigator_buntan"}],"since":"2003","until":"2005","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0017102"}],"notation":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"KYUSHU UNIVERSITY"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific Research"}]}],"url":[{"@id":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380027/","notation":[{"@value":"KAKEN"}]}],"createdAt":"2003-03-31","modifiedAt":"2025-11-20","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E7%90%83%E6%A0%B9%E4%BD%9C%E7%89%A9","dc:title":"球根作物"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%BC%91%E7%9C%A0","dc:title":"休眠"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%81%BA%E4%BC%9D%E5%AD%90","dc:title":"遺伝子"},{"@id":"https://cir.nii.ac.jp/all?q=bulbous%20plants","dc:title":"bulbous plants"},{"@id":"https://cir.nii.ac.jp/all?q=dormancy","dc:title":"dormancy"},{"@id":"https://cir.nii.ac.jp/all?q=genes","dc:title":"genes"}],"product":[{"@id":"https://cir.nii.ac.jp/crid/1010282256783025408","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Characterization of genes related to hyacinth bulb formation induced by low temperature or ABA."}]},{"@id":"https://cir.nii.ac.jp/crid/1010282256783025409","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"Search for the genes regulating bulb formation in Lilium speciosum Thunb."}]},{"@id":"https://cir.nii.ac.jp/crid/1010282256783025411","@type":"Article","resourceType":"学術雑誌論文(journal article)","notation":[{"@language":"ja","@value":"In-vitro bulb formation of seedlings in Allium scjoenoprasum L."}]},{"@id":"https://cir.nii.ac.jp/crid/1571698600422096000","@type":"Article","resourceType":"学術雑誌論文(journal article)","productIdentifier":[{"@type":"NAID","@value":"10019588907"}],"notation":[{"@language":"ja","@value":"In-vitro における Allium schoenoprasum L. 実生の球根形成について"},{"@language":"en","@value":"In-vitro bulb formation of seedlings in Allium schoenoprasum L."}]}],"dataSourceIdentifier":[{"@type":"KAKEN","@value":"15380027"}],"grant":{"grantIdentifier":[{"@type":"NII","@value":"69"}],"jpcoar:fundingStream":[{"@language":"ja","@value":"基盤研究(B)"},{"@language":"en","@value":"Grant-in-Aid for Scientific Research (B)"}]},"allocationClassification":[{"notation":[{"@language":"ja","@value":"補助金"},{"@language":"en","@value":"Single-year Grants"}]}],"projectStatus":"project_closed","allocationAmount":{"totalCost":{"amount":"15800000","currency":"JPY"},"breakdownCost":[{"notation":[{"@language":"ja","@value":"直接経費"},{"@language":"en","@value":"Direct Cost"}],"amount":"15800000","currency":"JPY"}]}}