Global regulatory mechanism of endocytosis revealed by cell-surface imaging technique

KAKEN

About This Project

Japan Grant Number
JP18H02436 (JGN)
Funding Program
Grants-in-Aid for Scientific Research
Funding Organization
Japan Society for the Promotion of Science

Kakenhi Information

Project/Area Number
18H02436
Research Category
Grant-in-Aid for Scientific Research (B)
Allocation Type
  • Single-year Grants
Review Section / Research Field
  • Basic Section 44010:Cell biology-related
Research Institution
  • Kyoto University
Project Period (FY)
2018-04-01 〜 2022-03-31
Project Status
Completed
Budget Amount*help
17,030,000 Yen (Direct Cost: 13,100,000 Yen Indirect Cost: 3,930,000 Yen)

Research Abstract

We established a correlative live-cell imaging system by combining high-speed atomic force microscopy (HS-AFM) and confocal laser-scanning microscopy and revealed the detailed morphological changes in the plasma membrane and protein localizations during the clathrin-mediated endocytosis. CIP4, which has membrane-deforming activity, is recruited to the plasma membrane via small G protein Cdc42, and promotes the assembly of actin, which induces a small membrane bulge near the clathrin pit. We also made an ultra-thin stretching cell chamber using polydimethylsiloxane and successfully installed into the above-mentioned correlative imaging system together with a thin stretching device. By using this live-cell stretching system, we analyzed how one-dimensional stretching stimulus affected the progress of clathrin-mediated endocytosis, and identified the tension-dependent steps in the initiation and closing steps.

Related Articles

See more

Related Data

See more

Related Books

See more

Related Dissertations

See more

Related Projects

See more

Related Products

See more

Keywords

Details 詳細情報について

Back to top