{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040282256976278656.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18K06294"},{"@type":"JGN","@value":"JP18K06294"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K06294/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"高いCO2吸収能力と乾燥耐性の両立を可能とする気孔応答の最適化メカニズム"},{"@language":"en","@value":"Optimization of stomatal regulation that enables both high CO2 absorption and drought tolarance"}],"field":[{"keyword":[{"language":"ja","textList":["小区分44030:植物分子および生理科学関連"]},{"language":"en","textList":["Basic Section 44030:Plant molecular biology and physiology-related"]}]}],"dc:language":"ja","description":[{"type":"outline_of_research_achievement","notation":[{"@language":"ja","@value":"気孔閉鎖を介したCO2吸収と水蒸散のバランス制御機構の解明をめざして、ゲノム編集による気孔閉鎖制御のキー因子であるSLAC1の構造改変を行った。その結果、ジーンターゲティングの手法により、イネの内在のSLAC1上の複数のCO2シグナル受信候補部位 (Y256/Y473) をピンポイントでアミノ酸置換する事に成功した。完成したSLAC1改変株SLAC1-Y256F_Y473Fを用いてガス交換測定による気孔応答を調べ、CO2応答が低下し、一方ABA応答は正常であることを確認した。このことはSLAC1の構造改変により、気孔のCO2応答を特異的にコントロールできることを示している。"},{"@language":"en","@value":"To elucidate the regulatory mechanism of the balance between CO2 absorption and water transpiration through stomatal closure, we structurally modified rice SLAC1 protein, a key factor in the regulation of stomatal closure, by homologous recombination-mediated genome editing. We succeeded in introducing two amino acid substitutions (Y256F/Y473F) on the SLAC1 gene that disrupt the candidate CO2 signal receiving site. Gas exchange measurement exhibited that in the SLAC1-modified plants, elevated CO2-induced stomatal closure was significantly reduced. By contrast, ABA-induced stomatal closure remained unaffected. These results indicate that stomatal CO2 response can be artificially controlled by modification of the SLAC1 structure."}]},{"type":"significance_of_research_achievement","notation":[{"@language":"ja","@value":"気孔細胞内で働く因子の構造を直接かつ精密に改変することにより、これまで不明であったCO2吸収と水蒸散のバランス制御機構を詳細に調べることが可能となった。また、大気中CO2濃度が増加し続ける中、食糧増産の観点から高CO2環境における光合成の効率低下が問題となっているが、本研究の成果は、高CO2環境下においてもCO2を効率的に利用できる植物開発の可能性につながる。本研究で用いた、ゲノム編集やジーンターゲティングなどの内在遺伝子を直接改変する技術により、今後は主要な育種技術の一つとして重要度が増すと考えられ、本研究の成果はそのさきがけとして位置づけられる。"}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1420282801181585536","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00304725"},{"@type":"NRID","@value":"1000000304725"},{"@type":"ORCID","@value":"0000-0001-8163-1899"},{"@type":"NRID","@value":"9000005144873"},{"@type":"NRID","@value":"9000364988839"},{"@type":"NRID","@value":"9000003048677"},{"@type":"NRID","@value":"9000017384930"},{"@type":"NRID","@value":"9000391951830"},{"@type":"NRID","@value":"9000364983220"},{"@type":"NRID","@value":"9000364984722"},{"@type":"NRID","@value":"9000364985041"},{"@type":"NRID","@value":"9000241962339"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0091812"}],"foaf:name":[{"@language":"ja","@value":"楠見 健介"},{"@language":"en","@value":"Kusumi Kensuke"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"}],"role":"principal_investigator"},{"@id":"https://cir.nii.ac.jp/crid/1420845751154203904","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70529099"},{"@type":"NRID","@value":"1000070529099"},{"@type":"NRID","@value":"9000391919660"},{"@type":"NRID","@value":"9000241962338"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/juntaro-negi"}],"foaf:name":[{"@language":"ja","@value":"祢宜 淳太郎"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"}],"role":"co_investigator_buntan"}],"since":"2018-04-01","until":"2022-03-31","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0017102"}],"notation":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific 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