{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1040282256981691648.json","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18K16894"},{"@type":"JGN","@value":"JP18K16894"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K16894/"}],"jpcoar:awardTitle":[{"@language":"ja","@value":"頭頸部癌における循環腫瘍DNAを用いたPrecision Medicineの確立"},{"@language":"en","@value":"Establishment of ctDNA monitoring system in HNSCC"}],"field":[{"keyword":[{"language":"ja","textList":["小区分56050:耳鼻咽喉科学関連"]},{"language":"en","textList":["Basic Section 56050:Otorhinolaryngology-related"]}]}],"dc:language":"ja","description":[{"type":"outline_of_research_achievement","notation":[{"@language":"ja","@value":"頭頸部癌26症例をSCC panelで変異解析を行い、同定された腫瘍特異的変異遺伝子をctDNA候補遺伝子と定義し、digital PCRを用いてctDNAモニタリングを行った。最も多く認められた変異はTP53であり、変異遺伝子を同定できなかった2例を除いた24例中15例（62.5%）で認められた。6例はdigital PCRが施行できず。継続的にctDNAモニタリングを施行可能であった残りの18例について解析を行った。7例は初回治療後ctDNAが陽性となり、7例全例再発もしくは腫瘍の残存を認めた。初回治療後ctDNAが陰性を維持している11例に比べて、陽性例は有意に予後不良であった。"},{"@language":"en","@value":"Mutation analysis of tumor and normal (PBMC) DNAs of 26 patients with HNSCC was performed using a custom SCC panel. The identified individualized mutated genes were defined as ctDNA candidates. We performed frequent ctDNA monitoring via digital PCR (dPCR). TP53 was the most frequently mutated gene and was detected in 14 of 24 cases (62.5%), wherein 2 cases were excluded owing to the absence of tumor-specific mutations in the SCC panel. Six cases were excluded because of undesignable and unusable primer-probes for dPCR. Longitudinal ctDNA was monitored in a total of 18 cases. In 7 cases, ctDNA tested positive again or did not tested negative, and all 7 cases relapsed after initial treatment. In 11 cases, after initial treatment, ctDNA remained negative and patients were alive without recurrence. Patients who remained negative for ctDNA during follow-up after initial treatment (n = 11) had a significantly better prognosis than those who reverted to ctDNA positivity (n = 7; P < 0.0001)."}]},{"type":"significance_of_research_achievement","notation":[{"@language":"ja","@value":"頭頸部癌には既存の腫瘍マーカーの測定についても有用性が確立されておらず、新規のバイオマーカーの同定が期待されている。ｃｔDNAはバイオマーカーとしての利用が期待されているが、頭頸部癌は変異スペクトラムが広く、特定の単一ctDNAをバイオマーカーとして使用することはできない。本手法によるctDNAモニタリングは頭頸部癌の臨床経過を鋭敏で反映していることがわかった。また、治療後に検出されるctDNAは再発や予後に寄与していることも判明した。今後、変異遺伝子のprimer-probeのライブラリの作成をすすめることでより簡易に安価でctDNAを臨床的バイオマーカーとして利用できる可能性がある。"}]}],"researcher":[{"@id":"https://cir.nii.ac.jp/crid/1420564276190752640","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90529885"},{"@type":"NRID","@value":"1000090529885"},{"@type":"NRID","@value":"9000345382019"},{"@type":"NRID","@value":"9000017289581"},{"@type":"NRID","@value":"9000388536676"},{"@type":"NRID","@value":"9000388536696"},{"@type":"NRID","@value":"9000311508497"},{"@type":"NRID","@value":"9000369747794"},{"@type":"NRID","@value":"9000321625869"},{"@type":"NRID","@value":"9000256834238"},{"@type":"NRID","@value":"9000258257873"},{"@type":"NRID","@value":"9000256834505"},{"@type":"NRID","@value":"9000311508492"},{"@type":"NRID","@value":"9000258257843"},{"@type":"NRID","@value":"9000402248667"},{"@type":"NRID","@value":"9000405101456"},{"@type":"NRID","@value":"9000406043136"},{"@type":"NRID","@value":"9000402264099"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/ryukogo"}],"foaf:name":[{"@language":"ja","@value":"古後 龍之介"},{"@language":"en","@value":"Kogo Ryunosuke"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"九州大学"}],"role":"principal_investigator"}],"since":"2018-04-01","until":"2022-03-31","institution":[{"institutionIdentifier":[{"@type":"NII","@value":"0017102"}],"notation":[{"@language":"ja","@value":"九州大学"},{"@language":"en","@value":"Kyushu University"}]}],"fundingProgram":[{"jpcoar:funderName":[{"@language":"ja","@value":"科学研究費助成事業"},{"@language":"en","@value":"Grants-in-Aid for Scientific Research"}]}],"url":[{"@id":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K16894/","notation":[{"@value":"KAKEN"}]}],"createdAt":"2018-04-23","modifiedAt":"2025-11-19","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=ctDNA","dc:title":"ctDNA"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%AA%E3%82%AD%E3%83%83%E3%83%89%E3%83%90%E3%82%A4%E3%82%AA%E3%83%97%E3%82%B7%E3%83%BC","dc:title":"リキッドバイオプシー"},{"@id":"https://cir.nii.ac.jp/all?q=%E9%A0%AD%E9%A0%B8%E9%83%A8%E7%99%8C","dc:title":"頭頸部癌"},{"@id":"https://cir.nii.ac.jp/all?q=liquid%20biopsy","dc:title":"liquid biopsy"}],"product":[{"@id":"https://cir.nii.ac.jp/crid/1360290617520097536","@type":"Article","resourceType":"学術雑誌論文(journal article)","productIdentifier":[{"@type":"DOI","@value":"10.1002/lary.29421"},{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/lary.29421"},{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lary.29421"},{"@type":"PMID","@value":"33538330"}],"notation":[{"@value":"Prognostic Significance of Systemic Inflammatory Response in Cases of Temporal Bone Squamous Cell Carcinoma"}]}],"dataSourceIdentifier":[{"@type":"KAKEN","@value":"18K16894"}],"grant":{"grantIdentifier":[{"@type":"NII","@value":"252"}],"jpcoar:fundingStream":[{"@language":"ja","@value":"若手研究"},{"@language":"en","@value":"Grant-in-Aid for Early-Career Scientists"}]},"allocationClassification":[{"notation":[{"@language":"ja","@value":"基金"},{"@language":"en","@value":"Multi-year Fund"}]}],"projectStatus":"project_closed","allocationAmount":{"totalCost":{"amount":"4160000","currency":"JPY"},"breakdownCost":[{"notation":[{"@language":"ja","@value":"直接経費"},{"@language":"en","@value":"Direct Cost"}],"amount":"3200000","currency":"JPY"},{"notation":[{"@language":"ja","@value":"間接経費"},{"@language":"en","@value":"Indirect Cost"}],"amount":"960000","currency":"JPY"}]}}