{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1050001202299973760.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"HDL","@value":"http://hdl.handle.net/2433/108609"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"6408846"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/6408846"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I6408846"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10864062"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"10864062"}},{"identifier":{"@type":"NAID","@value":"110003371918"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/10864062"}},{"identifier":{"@type":"DOI","@value":"10.2108/zsj.18.187"}},{"identifier":{"@type":"URI","@value":"http://www.bioone.org/doi/pdf/10.2108/zsj.18.187"}},{"identifier":{"@type":"URI","@value":"http://dl.ndl.go.jp/info:ndljp/pid/10864062"}},{"identifier":{"@type":"HANDLE","@value":"2433/108609"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"A Large-Scale Whole-Mount in situ Hybridization System: Rapid One-Tube Preparation of DIG-Labeled RNA Probes and High Throughput Hybridization using 96-Well Silent Screen Plates"},{"@language":"ja-Kana","@value":"large scale whole mount in situ hybridization system Rapid one tube preparation of DIG labeled RNA probes and high throughput hybridization using 96 well silent screen plates"},{"@value":"A Large-Scale Whole-Mount in situ Hybridization System : Rapid One-Tube Preparation of DIG-Labeled RNA Probes and High Throughput Hybridization using 96-Well Silent Screen Plates(Cell Biology)"},{"@value":"A Large-Scale Whole-Mount<i>in situ</i>Hybridization System: Rapid One-Tube Preparation of DIG-Labeled RNA Probes and High Throughput Hybridization using 96-Well Silent Screen Plates"}],"dc:language":"en","description":[{"type":"Abstract","notation":[{"@language":"en","@value":"Recent progress in multiple and automated-sequencing technology allows large-scale random cDNA sequencing, the so-called EST project, in various fields. In addition to the EST collection, the cDNA project requires analysis of spatiotemporal patterns of gene expression of a large number of clones by whole-mount in situ hybridization (WISH). To facilitate the multiple WISH procedures, we developed a protocol for rapid and uniform synthesis of multiple probes and multi-well based WISH processing. A DIG-labeled RNA probe for WISH was synthesized from a PCR-amplified template which contained an RNA promoter. All reactions of PCR and subsequent RNA synthesis were performed in a single tube by sequential addition of the reagents without phenol extraction or ethanol precipitation steps. An RNA probe was purified and condensed by a centrifugal ultrafilter to achieve high and stable purification efficiency. WISH of 96 samples were performed simultaneously in a 96-well plate attached to silent screen filters that were connected with a vacuum exhausting system. These processes eliminated the labor-intensive steps of WISH and provided opportunities to search for novel genes."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276169371648","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00343088"},{"@type":"NRID","@value":"1000000343088"},{"@type":"NRID","@value":"9000022307817"},{"@type":"NRID","@value":"9000010044614"},{"@type":"NRID","@value":"9000023428071"},{"@type":"NRID","@value":"9000392136505"},{"@type":"NRID","@value":"9000010061878"},{"@type":"NRID","@value":"9000017316911"},{"@type":"NRID","@value":"9000002745855"},{"@type":"NRID","@value":"9000007930477"},{"@type":"NRID","@value":"9000403737034"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0160970"}],"foaf:name":[{"@language":"en","@value":"Ogasawara, 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Takuya"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751136272384","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10400649"},{"@type":"NRID","@value":"1000010400649"},{"@type":"ORCID","@value":"0000-0001-7161-8367"},{"@type":"NRID","@value":"9000001821490"},{"@type":"NRID","@value":"9000016428580"},{"@type":"NRID","@value":"9000006630932"},{"@type":"NRID","@value":"9000253528095"},{"@type":"NRID","@value":"9000325821876"},{"@type":"NRID","@value":"9000410489857"},{"@type":"NRID","@value":"9000001692775"},{"@type":"NRID","@value":"9000260280717"},{"@type":"NRID","@value":"9000017736317"},{"@type":"NRID","@value":"9000415200538"},{"@type":"NRID","@value":"9000364746680"},{"@type":"NRID","@value":"9000413477643"},{"@type":"NRID","@value":"9000413477641"},{"@type":"NRID","@value":"9000071965740"},{"@type":"NRID","@value":"9000242104207"},{"@type":"NRID","@value":"9000413533845"},{"@type":"NRID","@value":"9000325821908"},{"@type":"NRID","@value":"9000414092099"},{"@type":"NRID","@value":"9000397685786"},{"@type":"NRID","@value":"9000021914111"},{"@type":"NRID","@value":"9000403041603"},{"@type":"NRID","@value":"9000364720671"},{"@type":"NRID","@value":"9000018879560"},{"@type":"NRID","@value":"9000413508381"},{"@type":"NRID","@value":"9000413508387"},{"@type":"NRID","@value":"9000297477046"},{"@type":"NRID","@value":"9000010223686"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0091998"}],"foaf:name":[{"@language":"en","@value":"Sasakura, 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