Suppression of mitochondrial oxygen metabolism mediated by the transcription factor HIF-1 alleviates propofol-induced cell toxicity

DOI HANDLE PDF Web Site 9 Citations 37 References Open Access
  • Sumi, Chisato
    Department of Anesthesiology, Kansai Medical University･Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Okamoto, Akihisa
    Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Tanaka, Hiromasa
    Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Kusunoki, Munenori
    Department of Anesthesiology, Kansai Medical University･Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Shoji, Tomohiro
    Department of Anesthesiology, Kansai Medical University･Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Uba, Takeo
    Department of Anesthesiology, Kansai Medical University･Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Adachi, Takehiko
    Department of Anesthesiology, Tazuke Kofukai Medical Research Institute
  • Iwai, Teppei
    Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Nishi, Kenichiro
    Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Harada, Hiroshi
    Laboratory of Cancer Cell Biology, Radiation Biology Center, Kyoto University･Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST)
  • Bono, Hidemasa
    Database Center for Life Science (DBCLS), Research Organization of Information and Systems (ROIS), Mishima
  • Matsuo, Yoshiyuki
    Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University
  • Hirota, Kiichi
    Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University

Abstract

A line of studies strongly suggest that the intravenous anesthetic, propofol, suppresses mitochondrial oxygen metabolism. It is also indicated that propofol induces the cell death in a reactive oxygen species (ROS)-dependent manner. Because hypoxia-inducible factor 1 (HIF-1) is a transcription factor which is involved in cellular metabolic reprogramming by modulating gene expressions of enzymes including glycolysis pathway and oxygen utilization of mitochondria, we examined the functional role of HIF-1 activity in propofol-induced cell death. The role of HIF-1 activity on oxygen and energy metabolisms and propofol-induced cell death and caspase activity was examined in renal cell-derived RCC4 cells: RCC4-EV cells which lack von Hippel-Lindau protein (VHL) protein expression and RCC4-VHL cells, which express exogenous VHL, and in neuronal SH-SY5Y cells. It was demonstrated that HIF-1 is involved in suppressing oxygen consumption and facilitating glycolysis in cells and that the resistance to propofol-induced cell death was established in a HIF-1 activation-dependent manner. It was also demonstrated that HIF-1 activation by treatment with HIFα-hydroxylase inhibitors such as n-propyl gallate and dimethyloxaloylglycine, alleviated the toxic effects of propofol. Thus, the resistance to propofol toxicity was conferred by HIF-1 activation by not only genetic deletion of VHL but also exposure to HIFα-hydroxylase inhibitors.

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