{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1050004953596614912.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"URI","@value":"https://ousar.lib.okayama-u.ac.jp/60981"}},{"identifier":{"@type":"DOI","@value":"10.7717/peerj.10244"}},{"identifier":{"@type":"URI","@value":"https://peerj.com/articles/10244.pdf"}},{"identifier":{"@type":"URI","@value":"https://peerj.com/articles/10244.xml"}},{"identifier":{"@type":"URI","@value":"https://peerj.com/articles/10244.html"}},{"identifier":{"@type":"PMID","@value":"33240612"}},{"identifier":{"@type":"NAID","@value":"120006937507"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes"}],"dc:language":"en","description":[{"type":"Other","notation":[{"@value":"Background</br>\nIn this study, we investigated the effect of the mechanical loading history on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) in MLO-Y4 osteocyte-like cells.</br>\nMethods</br>\nThree hours after MLO-Y4 osteocytes were seeded, a continuous compressive force (CCF) of 31 dynes/cm2 with or without additional CCF (32 dynes/cm2) was loaded onto the osteocytes. After 36 h, the additional CCF (loading history) was removed for a recovery period of 10 h. The expression of RANKL, OPG, RANKL/OPG ratio, cell numbers, viability and morphology were time-dependently examined at 0, 3, 6 and 10 h. Then, the same additional CCF was applied again for 1 h to all osteocytes with or without the gap junction inhibitor to examine the expression of RANKL, OPG, the RANKL/OPG ratio and other genes that essential to characterize the phenotype of MLO-Y4 cells. Fluorescence recovery after photobleaching technique was also applied to test the differences of gap-junctional intercellular communications (GJIC) among MLO-Y4 cells.</br>\nResults</br>\nThe expression of RANKL and OPG by MLO-Y4 osteocytes without a loading history was dramatically decreased and increased, respectively, in response to the 1-h loading of additional weight. However, the expression of RANKL, OPG and the RANKL/OPG ratio were maintained at the same level as in the control group in the MLO-Y4 osteocytes with a loading history but without gap junction inhibitor treatment. Treatment of loading history significantly changed the capacity of GJIC and protein expression of connexin 43 (Cx43) but not the mRNA expression of Cx43. No significant difference was observed in the cell number or viability between the MLO-Y4 osteocyte-like cells with and without a loading history or among different time checkpoints during the recovery period. The cell morphology showed significant changes and was correlated with the expression of OPG, Gja1 and Dmp1 during the recovery period.</br>\nConclusion</br>\nOur findings indicated that the compressive force-induced changes in the RANKL/OPG expression could be habituated within at least 11 h by 36-h CCF exposure. GJIC and cell morphology may play roles in response to loading history in MLO-Y4 osteocyte-like cells."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1070004953596614915","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000410999805"}],"foaf:name":[{"@language":"en","@value":"Wang, Ziyi"}]},{"@id":"https://cir.nii.ac.jp/crid/1070004953596614916","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000410999806"}],"foaf:name":[{"@language":"en","@value":"Weng, Yao"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751165268480","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70549881"},{"@type":"NRID","@value":"1000070549881"},{"@type":"NRID","@value":"9000002445039"},{"@type":"NRID","@value":"9000253672315"},{"@type":"NRID","@value":"9000006050033"},{"@type":"NRID","@value":"9000259071211"},{"@type":"NRID","@value":"9000368412703"},{"@type":"NRID","@value":"9000413565975"},{"@type":"NRID","@value":"9000410999807"},{"@type":"NRID","@value":"9000254480955"},{"@type":"NRID","@value":"9000024817438"},{"@type":"NRID","@value":"9000024207854"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/7000027113"}],"foaf:name":[{"@language":"en","@value":"Ishihara, Yoshihito"}]},{"@id":"https://cir.nii.ac.jp/crid/1070004953596614917","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000410999808"}],"foaf:name":[{"@language":"en","@value":"Odagaki, Naoya"}]},{"@id":"https://cir.nii.ac.jp/crid/1070004953596614913","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000410999809"}],"foaf:name":[{"@language":"en","@value":"Hlaing, Ei Ei Hsu"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751138441344","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30380017"},{"@type":"NRID","@value":"1000030380017"},{"@type":"NRID","@value":"9000415170703"},{"@type":"NRID","@value":"9000413743153"},{"@type":"NRID","@value":"9000254883058"},{"@type":"NRID","@value":"9000378106628"},{"@type":"NRID","@value":"9000403962624"},{"@type":"NRID","@value":"9000403166515"},{"@type":"NRID","@value":"9000018149594"},{"@type":"NRID","@value":"9000410999810"},{"@type":"NRID","@value":"9000413743195"},{"@type":"NRID","@value":"9000411500379"},{"@type":"NRID","@value":"9000002155120"},{"@type":"NRID","@value":"9000401422461"},{"@type":"NRID","@value":"9000398801031"},{"@type":"NRID","@value":"9000403962646"},{"@type":"NRID","@value":"9000403971893"},{"@type":"NRID","@value":"9000403962639"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0088290"}],"foaf:name":[{"@language":"en","@value":"Izawa, Takashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801183940480","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20380024"},{"@type":"NRID","@value":"1000020380024"},{"@type":"NRID","@value":"9000414243848"},{"@type":"NRID","@value":"9000397426839"},{"@type":"NRID","@value":"9000001545952"},{"@type":"NRID","@value":"9000004579439"},{"@type":"NRID","@value":"9000409467881"},{"@type":"NRID","@value":"9000410999811"},{"@type":"NRID","@value":"9000253184109"},{"@type":"NRID","@value":"9000002155163"},{"@type":"NRID","@value":"9000387467698"},{"@type":"NRID","@value":"9000006629884"},{"@type":"NRID","@value":"9000405632411"},{"@type":"NRID","@value":"9000397712051"},{"@type":"NRID","@value":"9000409314274"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0066654"}],"foaf:name":[{"@language":"en","@value":"Okamura, Hirohiko"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801190299008","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80253219"},{"@type":"NRID","@value":"1000080253219"},{"@type":"NRID","@value":"9000308715268"},{"@type":"NRID","@value":"9000313611374"},{"@type":"NRID","@value":"9000303644367"},{"@type":"NRID","@value":"9000330667905"},{"@type":"NRID","@value":"9000335564877"},{"@type":"NRID","@value":"9000369737289"},{"@type":"NRID","@value":"9000368412705"},{"@type":"NRID","@value":"9000409467816"},{"@type":"NRID","@value":"9000000485094"},{"@type":"NRID","@value":"9000413565978"},{"@type":"NRID","@value":"9000240212232"},{"@type":"NRID","@value":"9000316696176"},{"@type":"NRID","@value":"9000409496023"},{"@type":"NRID","@value":"9000024297575"},{"@type":"NRID","@value":"9000297499567"},{"@type":"NRID","@value":"9000411380169"},{"@type":"NRID","@value":"9000012275781"},{"@type":"NRID","@value":"9000412202536"},{"@type":"NRID","@value":"9000412303438"},{"@type":"NRID","@value":"9000409579917"},{"@type":"NRID","@value":"9000410999812"},{"@type":"NRID","@value":"9000279213845"},{"@type":"NRID","@value":"9000413743200"},{"@type":"NRID","@value":"9000405916303"},{"@type":"NRID","@value":"9000010303464"},{"@type":"NRID","@value":"9000024817437"},{"@type":"NRID","@value":"9000403425051"},{"@type":"NRID","@value":"9000331418146"},{"@type":"NRID","@value":"9000398428425"},{"@type":"NRID","@value":"9000398421276"},{"@type":"NRID","@value":"9000305612242"},{"@type":"NRID","@value":"9000298264831"},{"@type":"NRID","@value":"9000403036655"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0184043"}],"foaf:name":[{"@language":"en","@value":"Kamioka, Hiroshi"}]}],"publication":{"publicationIdentifier":[{"@type":"ISSN","@value":"21678359"},{"@type":"EISSN","@value":"21678359"}],"prism:publicationName":[{"@value":"PeerJ"}],"dc:publisher":[{"@value":"PeerJ"}],"prism:publicationDate":"2020-11-09","prism:volume":"8","prism:startingPage":"e10244"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["© 2020 Wang et al."],"url":[{"@id":"https://ousar.lib.okayama-u.ac.jp/60981"},{"@id":"https://peerj.com/articles/10244.pdf"},{"@id":"https://peerj.com/articles/10244.xml"},{"@id":"https://peerj.com/articles/10244.html"},{"@id":"http://ousar.lib.okayama-u.ac.jp/60981"}],"foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Osteocytes","dc:title":"Osteocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Habituation","dc:title":"Habituation"},{"@id":"https://cir.nii.ac.jp/all?q=18%20alpha-Glycyrrhetinic%20acid","dc:title":"18 alpha-Glycyrrhetinic acid"},{"@id":"https://cir.nii.ac.jp/all?q=Fluorescence%20recovery%20after%20photobleaching","dc:title":"Fluorescence recovery after photobleaching"},{"@id":"https://cir.nii.ac.jp/all?q=Gap%20junctional%20intercellular%20communication","dc:title":"Gap junctional intercellular communication"}],"dcterms:subject":[{"subjectScheme":"Other","notation":[{"@value":"Osteocytes"}]},{"subjectScheme":"Other","notation":[{"@value":"Habituation"}]},{"subjectScheme":"Other","notation":[{"@value":"18 alpha-Glycyrrhetinic acid"}]},{"subjectScheme":"Other","notation":[{"@value":"Fluorescence recovery after photobleaching"}]},{"subjectScheme":"Other","notation":[{"@value":"Gap junctional intercellular communication"}]}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781993563520","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18H03011"},{"@type":"JGN","@value":"JP18H03011"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H03011/"}],"notation":[{"@language":"ja","@value":"顎顔面領域骨代謝性疾患におけるエピゲノム制御による新規骨リモデリング機構の解明"},{"@language":"en","@value":"Epigenetic analysis of new bone remodeling mechanism in the dentofacial region."}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782006699648","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18KK0464"},{"@type":"JGN","@value":"JP18KK0464"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KK0464/"}],"notation":[{"@language":"ja","@value":"顎顔面領域稀少性遺伝子疾患における骨代謝制御機構とその破綻のエピゲノム解析"},{"@language":"en","@value":"Epigenetic analysis of regulating bone and mineral metabolism under rare disease in orthognathic lesion"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256934556032","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"17H04413"},{"@type":"JGN","@value":"JP17H04413"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04413/"}],"notation":[{"@language":"ja","@value":"骨細胞性骨溶解を基軸とした歯牙移動制御機構の解明"},{"@language":"en","@value":"Regulation of tooth movement-initiated mechanotransduction via osteocytic bone remodeling"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256991588224","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19H03859"},{"@type":"JGN","@value":"JP19H03859"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03859/"}],"notation":[{"@language":"ja","@value":"生体ボリュームイメージ解析を用いた骨細胞のメカノセンサー機能獲得過程の解明"},{"@language":"en","@value":"Elucidation of mechanosensor function acquisition process of osteocytes using 3D-volumetric image data"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256991671040","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19H04051"},{"@type":"JGN","@value":"JP19H04051"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H04051/"}],"notation":[{"@language":"ja","@value":"歯周病原菌が放出する小胞の組織障害性と病態評価への応用"},{"@language":"en","@value":"The role of extracellular vesicles of oral bacteria in systemic disease"}]},{"@id":"https://cir.nii.ac.jp/crid/1040848250603783296","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19J11906"},{"@type":"JGN","@value":"JP19J11906"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19J11906/"}],"notation":[{"@language":"ja","@value":"歯根膜と歯槽骨のクロストークにおける概日リズムを介した骨細胞制御機構の解明"},{"@language":"en","@value":"Investigating the mechanism to regulate osteocytes by circadian rhythm that effected by the crosstalk with periodontal ligament cells"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050002212548533504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts"}]},{"@id":"https://cir.nii.ac.jp/crid/1050570699974137088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Changes in the intra- and peri-cellular sclerostin distribution in lacuno-canalicular system induced by mechanical unloading"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002215834849280","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Alternation in the gap-junctional intercellular communication capacity during the maturation of osteocytes in the embryonic chick calvaria"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002216808190336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evidence for osteocyte regulation of bone homeostasis through RANKL expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143893299584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Enhanced Osteoclastic Resorption and Responsiveness to Mechanical Load in Gap Junction Deficient Bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143914435584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Do Bone Cells Behave Like a Neuronal Network?"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144871691520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Round versus flat: Bone cell morphology, elasticity, and mechanosensing"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145037991552","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Hormonal, pH, and Calcium Regulation of Connexin 43–Mediated Dye Transfer in Osteocytes in Chick Calvaria"}]},{"@id":"https://cir.nii.ac.jp/crid/1360282588964379904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biomedical Image Processing"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706255779712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Screening of key candidate genes and pathways for osteocytes involved in the differential response to different types of mechanical stimulation using a bioinformatics analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707243602944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Osteocyte function under compressive mechanical force"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285714664812800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Circulating osteocyte-derived exosomes contain miRNAs which are enriched in exosomes from MLO-Y4 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619293406848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The amazing osteocyte"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620924437248","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Osteocyte lacunae tissue strain in cortical bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621488484096","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Connexin 43 Is Required for the Anti-Apoptotic Effect of Bisphosphonates on Osteocytes and Osteoblasts In Vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165788186496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The early mouse 3D osteocyte network in the presence and absence of mechanical loading"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565166830227840","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Microscale fluid flow analysis in a human osteocyte canaliculus using a realistic high-resolution image-based three-dimensional model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574092892962688","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Fiji: an open-source platform for biological-image analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574093858416896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The potential role of spectrin network in the mechanotransduction of MLO-Y4 osteocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095678208896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Quantifying the strain history of bone: spatial uniformity and self-similarity of low-magnitude strains"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096344267008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Beyond gap junctions: Connexin43 and bone cell signaling"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846641368750848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Morphological aspects of the biological function of the osteocytic lacunar canalicular system and of osteocyte‐derived factors"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846645578552192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nuclear Actin in Development and Transcriptional Reprogramming"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568624878208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Three rules for bone adaptation to mechanical stimuli"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569289196160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A scalable pipeline for designing reconfigurable organisms"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571064800384","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Efficient Behavior of Small-World Networks"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571247049088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gene expression signatures of a fibroblastoid preosteoblast and cuboidal osteoblast cell model compared to the MLO-Y4 osteocyte cell model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571384310528","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Interaction of Biological Factors with Mechanical Signals in Bone Adaptation: Recent Developments"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869854350942208","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"<i>O</i>\n                    ‐\n                    <scp>GlcNAcylation</scp>\n                    regulates osteoblast differentiation through the morphological changes in mitochondria, cytoskeleton, and endoplasmic reticulum"}]},{"@id":"https://cir.nii.ac.jp/crid/1361131421495066368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cathepsin K–deficient osteocytes prevent lactation-induced bone loss and parathyroid hormone suppression"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137043606537344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Enhanced Periosteal and Endocortical Responses to Axial Tibial Compression Loading in Conditional Connexin43 Deficient Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044014320128","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanotransduction and functional response of the skeleton to physical stress: The mechanisms and mechanics of bone adaptation*"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044792613888","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Why Rest Stimulates Bone Formation: A Hypothesis Based on Complex Adaptive Phenomenon"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518884272128","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Architecture of the osteocyte network correlates with bone material quality"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519362396544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Exploring complex networks"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519985905024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Computational Investigation on the Biomechanical Responses of the Osteocytes to the Compressive Stimulus: A Poroelastic Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521098051456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cell autonomous requirement of connexin 43 for osteocyte survival: Consequences for endocortical resorption and periosteal bone formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996281366144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Fluorescence Photobleaching Assay of Gap Junction-mediated Communication Between Human Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468647415680","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Strain amplification in bone mechanobiology: a computational investigation of the\n                    <i>in vivo</i>\n                    mechanics of osteocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471024353536","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Phosphorylation of Connexin43 on Serine368 by Protein Kinase C Regulates Gap Junctional Communication"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945074515456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Calvarial and limb bone cells in organ and monolayer culture do not show the same early responses to dynamic mechanical strain"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945832501376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Establishment of an Osteocyte-like Cell Line, MLO-Y4"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419317382144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Connexin 43-mediated modulation of polarized cell movement and the directional migration of cardiac neural crest cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420141596672","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420363994624","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The role of the gap junction protein connexin43 in B lymphocyte motility and migration"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544421137334912","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Demonstration of osteocytic perilacunar/canalicular remodeling in mice during lactation"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893958978304","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular mechanosensors in osteocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894829512960","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Oscillating fluid flow activation of gap junction hemichannels induces atp release from MLO‐Y4 osteocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895344407040","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Osteocyte: An Endocrine Cell … and More"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895564258560","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Inhibitors of connexin and pannexin channels as potential therapeutics"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369656857984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Osteocytes, not Osteoblasts or Lining Cells, are the Main Source of the RANKL Required for Osteoclast Formation in Remodeling Bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107371370874496","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Measurement of Dynamic Protein Binding to Chromatin In Vivo, Using Photobleaching Microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844121070592","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Osteoblast and osteocyte: Games without frontiers"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844733685120","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Matrix-embedded cells control osteoclast formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845471144704","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Sclerostin is a delayed secreted product of osteocytes that inhibits bone formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845975940864","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The role of osteocytes in functional bone adaptation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846043851392","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Connexin 43 controls the multipolar phase of neuronal migration to the cerebral cortex"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320176383872","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Relationships of loading history and structural and material characteristics of bone: Development of the mule deer calcaneus"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320222783488","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Connexin43 and the Intercellular Signaling Network Regulating Skeletal Remodeling"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793314690944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Periosteal Bone Surface is Less Mechano-Responsive than the Endocortical"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793517586304","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Regulation of gap junction intercellular communication by connexin ubiquitination: physiological and pathophysiological implications"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793635401216","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanotransduction and the functional response of bone to mechanical strain"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794456805888","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Absence of Cx43 selectively from osteocytes enhances responsiveness to mechanical force in mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795118283392","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Impact of flow shear stress on morphology of osteoblast-like IDG-SW3 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795856729472","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanical Roles of F-Actin in the Differentiation of Stem Cells: A Review"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268909013632","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Modulation of membrane traffic by mechanical stimuli"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268936066176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Habituation Is More Than Learning to Ignore: Multiple Mechanisms Serve to Facilitate Shifts in Behavioral Strategy"}]},{"@id":"https://cir.nii.ac.jp/crid/1883962441230260352","@type":"Dataset","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes"}]}],"dataSourceIdentifier":[{"@type":"IRDB","@value":"oai:irdb.nii.ac.jp:01081:0004673952"},{"@type":"CROSSREF","@value":"10.7717/peerj.10244"},{"@type":"CIA","@value":"120006937507"},{"@type":"KAKEN","@value":"PRODUCT-23541029"},{"@type":"KAKEN","@value":"PRODUCT-23402836"},{"@type":"KAKEN","@value":"PRODUCT-23524786"},{"@type":"KAKEN","@value":"PRODUCT-23541809"},{"@type":"KAKEN","@value":"PRODUCT-23564027"},{"@type":"KAKEN","@value":"PRODUCT-23510943"},{"@type":"OPENAIRE","@value":"doi_dedup___::5fd6a4454ed9f748cabee49bea75aa7f"},{"@type":"CROSSREF","@value":"10.1002/biof.2131_references_DOI_47biTX1HzZz8naST5aT3wJh3r4t"}]}