Impacts of manipulating cell sorting on in vitro hair follicle regeneration

DOI 機関リポジトリ 機関リポジトリ (HANDLE) Web Site Web Site ほか2件をすべて表示 一部だけ表示 被引用文献5件 参考文献23件 オープンアクセス
  • Kageyama, Tatsuto
    Fac Engn, Yokohama National University Fac Engn, Kanagawa Academy Science & Technology
  • Anakama, Riki
    Fac Engn, Yokohama National University
  • Togashi, Hideru
    Fac Engn, Japan Sci & Technol Agcy JST PRESTO Fac Engn, Dept Biochem & Mol Biol, Div Mol & Cellular Biol, Grad Sch Med, Kobe University
  • Fukuda, Junji
    Fac Engn, Yokohama National University Fac Engn, Kanagawa Academy Science & Technology

書誌事項

公開日
2022-12-03
資源種別
journal article
DOI
  • 10.1016/j.jbiosc.2022.09.004
公開者
Society for Biotechnology, Japan

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説明

Hair follicle morphogenesis is triggered by epithelialemesenchymal interactions. Several approaches have been developed for preparing hair follicle organoids using epithelial and mesenchymal cells; however, the current understanding of the relevance of in vitro spontaneous organization processes to hair regeneration is limited. In the present study, we used Y27632, a rho-associated kinase inhibitor, to investigate the effects of manipulation of cell sorting on hair regeneration in vitro. Dissociated hair follicle-inducible epithelial and mesenchymal cells were cultured in Y27632containing media in 96-well plates or polydimethylsiloxane microarray plates. We found that Y27632 supplementation modulated the spatial distribution of epithelial and mesenchymal cells from a dumbbell shape to a coreeshell configuration via a spontaneous organization process. New hair follicles with typical morphological features emerged in the Y27632-treated core-shell-shaped aggregates, and hair shafts sprouted with approximately 100% efficiency in vitro. Gene chip analysis and pathway-inhibition experiments revealed that the phosphatidylinositol-3-kinase/protein kinase B- and Ras-signaling pathways were involved in hair-like sprouting in the Y27632-treated hair follicle organoids. Our findings enhance the understanding of hair follicle organogenesis and the development of hair follicle organoids. (c) 2022, The Society for Biotechnology, Japan. All rights reserved.

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