Fundamental Study on Novel Biological Indicator Using DNA-Labeled Microbeads for Evaluating Nonthermal Plasma Sterilization

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  • Nakano, Michihiko
    Department of Electrical Engineering, Faculty of Information Science and Electrical Engineering, Kyushu University
  • Okumura, Takamasa
    Department of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University
  • Inaba, Masafumi
    Department of Electrical Engineering, Faculty of Information Science and Electrical Engineering, Kyushu University
  • Attri, Pankaj
    Department of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University
  • Koga, Kazunori
    Department of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University
  • Shiratani, Masaharu
    Department of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University
  • Suehiro, Junya
    Department of Electrical Engineering, Faculty of Information Science and Electrical Engineering, Kyushu University

Bibliographic Information

Published
2024-06-28
Resource Type
journal article
Rights Information
  • © 2024 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
DOI
  • 10.1109/lsens.2024.3420437
Publisher
Institute of Electrical and Electronics Engineers (IEEE)

Description

Nonthermal atmospheric-pressure discharge plasma is considered important for sterilization. Reactive species, such as active oxygen species, radicals, and nitrate ions, generated by the discharge plasma damage the target bacterial cell wall/membrane and DNA. Several plasma sterilization methods have been proposed, including dielectric barrier discharge (DBD). To achieve effective sterilization, it is necessary to evaluate their characteristics using many parameters. This letter aims to demonstrate a proof-of-concept of a novel biological indicator for plasma sterilization. A biological indicator is used to verify sterilization outcomes. We employ DNA-labeled microbeads as biological indicators for the rapid visualization of plasma sterilization. This is based on our recently developed method for visual detection of DNA molecules. If plasma-derived factors cause the degradation of the DNA attached to the microbeads, this can be confirmed by visualization. Herein, we present the correlation between sterilization and visualization in the case of DBD. This method offers a rapid evaluation of plasma sterilization because it easily and quickly determines the sterilization capability of the plasma.

Journal

  • IEEE Sensors Letters

    IEEE Sensors Letters 8 (8), 4501904-, 2024-06-28

    Institute of Electrical and Electronics Engineers (IEEE)

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