Encapsulation of Axillary Buds of Gmelina arborea Roxb. and Peronema canescens Jack.

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  • Gmelina arborea Roxb.とPeronema canescens Jack.の腋芽のカプセル化

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This study developed a method of synthetic seed formation by encapsulating axillary buds from in vitro grown plantlets of two fast-growing tropical tree species, Gmelina arborea and Peronema canescens, under aseptic conditions. MS medium containing plant growth regulators (0.22 mg/l 6-benzylaminopurine, 0.02 mg/l alpha-naphthylacetic acid, and 1.00 mg/l 3-indolebuthylic acid) and different concentrations of sucrose (3, 6, 12, 24, and 36%) were used as the encapsulation medium in combination with 4% (w/v) sodium alginate. Nodal segments of plantlets were encapsulated with calcium alginate gel. Synthetic seeds were cultured on five types of agar medium containing different shoot sprouting components. Both G. arborea and P. canescens were successfully sprouted from axillary buds in any combination of encapsulation medium and shoot elongation medium after 6 weeks of culture. The MS shoot sprouting medium containing plant growth regulators and 3% sucrose appeared to be suitable for sprouting G. arborea shoots. Shoot sprouting of P. canescens was prominent on medium containing only plant growth regulators, indicating that a continuous supply of plant growth regulators is necessary for P. canescens shoot sprouting. Shoot elongation in G. arborea was promoted at lower sucrose concentrations in the encapsulation medium, whereas that of P. canescens was promoted at higher sucrose concentrations. Plants of these species were established within 1 month by transplanting the elongated shoots into vermiculite. When synthetic seeds were stored at different temperatures (4 10, 15, 20, and 25 deg C) and for different durations (1, 2, 3, and 4 weeks), they were successfully preserved at all combinations of temperature and duration except for those stored at 4 deg C. Preserved G. arborea synthetic seeds successfully sprouted shoots in a succeeding culture at 25 deg C, whereas P. canescens synthetic seeds did not sprout, although the explants remained green in color.



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