Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs

DOI 機関リポジトリ 機関リポジトリ (HANDLE) Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献2件 参考文献13件 オープンアクセス
  • Kanno, Seiya
    Fac Engn, Yokohama National University Fac Engn, Ctr Biol Safety & Res, Div Cellular & Mol Toxicol, National Institute of Health Sciences - Japan
  • Mizota, Kashu
    Fac Engn, Yokohama National University
  • Okubo, Yusuke
    Fac Engn, Ctr Biol Safety & Res, Div Cellular & Mol Toxicol, National Institute of Health Sciences - Japan
  • Kageyama, Tatsuto
    Fac Engn, Yokohama National University Fac Engn, Ctr Biol Safety & Res, Div Cellular & Mol Toxicol, Kanagawa Inst Ind Sci & Technol KISTEC
  • Yan, Lei
    Fac Engn, Yokohama National University
  • Fukuda, Junji
    Fac Engn, Yokohama National University Fac Engn, Ctr Biol Safety & Res, Div Cellular & Mol Toxicol, Kanagawa Inst Ind Sci & Technol KISTEC

書誌事項

公開日
2022-06-17
資源種別
journal article
権利情報
  • Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
DOI
  • 10.1016/j.xpro.2022.101439
公開者
Elsevier

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説明

We describe a protocol for a live-cell luciferase assay system for continuously monitoring fibroblast growth factor (FGF) signal disruption in human-induced pluripotent stem cells (iPSCs). Signal disrupting effects of chemicals are used as an indicator to evaluate toxicity. The assay is reliably predictive of the effects of limb malformation chemicals (AUC = 0.93). The current approach is limited to FGF signal disruption, and combinations with other types of signaling will be required to detect the effects of different toxicants. For complete details on the use and execution of this protocol, please refer to Kanno et al. (2022a).

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