{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1050585407777800960.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"HDL","@value":"http://hdl.handle.net/2433/293384"}},{"identifier":{"@type":"DOI","@value":"10.1002/edn3.406"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.406"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Environmental DNA extraction method from water for a high and consistent DNA yield"},{"@value":"Environmental <scp>DNA</scp> extraction method from water for a high and consistent <scp>DNA</scp> yield"}],"dc:language":"en","description":[{"type":"Abstract","notation":[{"@language":"en","@value":"Environmental DNA (eDNA) measurements have been widely applied in the biomonitoring of organisms. However, different DNA extraction methods may cause changes in yield and consistency, resulting in an inaccurate estimation of eDNA, especially when quantitative measurements are performed. This study focused on evaluating the effects of DNA extraction methods on the magnitude and consistency of fish DNA yield from water samples. Samples were collected periodically over a year from river and lake water systems, and reference eDNA was spiked into them. The spiked samples were extracted using three methods: using lysis buffer for blood and cells (AL method) with the DNeasy Blood and Tissue Kit (Qiagen), using lysis buffer for tissue (ATL method), and the microfluidic-channel method (BC method). DNA yields were compared by determining the copy number using qPCR. The ATL method showed better stability and a higher yield than the AL method. In addition, despite being comparatively simple, the BC method performed extractions stably and at relatively high yields. Based on the long-term evaluation of eDNA measurements using various methods, we showed that differences in DNA extraction methods may alter DNA yield and stability."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1070585407777800963","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Shirakura, Hiromi"}]},{"@id":"https://cir.nii.ac.jp/crid/1070585407777800961","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Fukuzawa, Takashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1070585407777800965","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Nishizawa, Naofumi"}]},{"@id":"https://cir.nii.ac.jp/crid/1070585407777800962","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Nagata, Hisao"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326236368768","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80608505"},{"@type":"NRID","@value":"1000080608505"},{"@type":"ORCID","@value":"0000-0002-2701-3982"},{"@type":"CINII_AUTHOR_ID","@value":"DA18476204"},{"@type":"URI","@value":"https://ci.nii.ac.jp/author/DA18476204#entity"},{"@type":"URI","@value":"https://viaf.org/viaf/NII%7CDA18476204"},{"@type":"NRID","@value":"9000006090249"},{"@type":"NRID","@value":"9000408785454"},{"@type":"NRID","@value":"9000283788313"},{"@type":"NRID","@value":"9000283789670"},{"@type":"NRID","@value":"9000292883861"},{"@type":"NRID","@value":"9000258529593"},{"@type":"NRID","@value":"9000002038213"},{"@type":"NRID","@value":"9000409848102"},{"@type":"NRID","@value":"9000258528628"},{"@type":"NRID","@value":"9000283126584"},{"@type":"NRID","@value":"9000252740921"},{"@type":"NRID","@value":"9000272259428"},{"@type":"NRID","@value":"9000283788415"},{"@type":"NRID","@value":"9000258529556"},{"@type":"NRID","@value":"9000010206676"},{"@type":"NRID","@value":"9000386338921"},{"@type":"NRID","@value":"9000403552052"},{"@type":"NRID","@value":"9000392029721"},{"@type":"NRID","@value":"9000255920000"},{"@type":"NRID","@value":"9000258486803"},{"@type":"NRID","@value":"9000380424809"},{"@type":"NRID","@value":"9000000616676"},{"@type":"NRID","@value":"9000267810099"},{"@type":"NRID","@value":"9000408576032"},{"@type":"NRID","@value":"9000351085423"},{"@type":"NRID","@value":"9000351085419"},{"@type":"NRID","@value":"9000383071834"},{"@type":"NRID","@value":"9000283789404"},{"@type":"NRID","@value":"9000002110995"},{"@type":"NRID","@value":"9000391399871"},{"@type":"NRID","@value":"9000283127296"},{"@type":"NRID","@value":"9000258528802"},{"@type":"NRID","@value":"9000244892434"},{"@type":"NRID","@value":"9000014158263"},{"@type":"NRID","@value":"9000391399903"},{"@type":"NRID","@value":"9000258528620"},{"@type":"NRID","@value":"9000356889216"},{"@type":"NRID","@value":"9000002163034"},{"@type":"NRID","@value":"9000412001526"},{"@type":"NRID","@value":"9000258529421"},{"@type":"NRID","@value":"9000017193568"},{"@type":"NRID","@value":"9000369737018"},{"@type":"NRID","@value":"9000283788322"},{"@type":"NRID","@value":"9000392025656"},{"@type":"NRID","@value":"9000258529183"},{"@type":"NRID","@value":"9000258527842"},{"@type":"NRID","@value":"9000258528537"},{"@type":"NRID","@value":"9000258528624"},{"@type":"NRID","@value":"9000019236438"},{"@type":"NRID","@value":"9000024158513"},{"@type":"NRID","@value":"9000351085393"},{"@type":"NRID","@value":"9000391399899"},{"@type":"NRID","@value":"9000258528592"},{"@type":"NRID","@value":"9000406184553"},{"@type":"NRID","@value":"9000369353644"},{"@type":"NRID","@value":"9000351085399"},{"@type":"NRID","@value":"9000256746020"},{"@type":"NRID","@value":"9000283127230"},{"@type":"NRID","@value":"9000415328629"},{"@type":"NRID","@value":"9000283788104"},{"@type":"NRID","@value":"9000258528799"},{"@type":"NRID","@value":"9000402502256"},{"@type":"NRID","@value":"9000386338865"},{"@type":"NRID","@value":"9000283788507"},{"@type":"NRID","@value":"9000258528597"},{"@type":"NRID","@value":"9000010444531"},{"@type":"NRID","@value":"9000356611102"},{"@type":"NRID","@value":"9000391399882"},{"@type":"NRID","@value":"9000347063874"},{"@type":"NRID","@value":"9000392028577"},{"@type":"NRID","@value":"9000324664297"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/hideyuki.doi"}],"foaf:name":[{"@language":"en","@value":"Doi, Hideyuki"},{"@language":"ja","@value":"土居, 秀幸"}]},{"@id":"https://cir.nii.ac.jp/crid/1070585407777800964","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Kameda, Yuichi"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"26374943"},{"@type":"EISSN","@value":"26374943"}],"prism:publicationName":[{"@language":"en","@value":"Environmental DNA"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2023-07","prism:volume":"5","prism:number":"4","prism:startingPage":"627","prism:endingPage":"633"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["© 2023 The Authors. Environmental DNA published by John Wiley & Sons Ltd.","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],"url":[{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.406"}],"foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Biryu-chip","dc:title":"Biryu-chip"},{"@id":"https://cir.nii.ac.jp/all?q=DNeasy%20extraction%20kit","dc:title":"DNeasy extraction kit"},{"@id":"https://cir.nii.ac.jp/all?q=eDNA","dc:title":"eDNA"},{"@id":"https://cir.nii.ac.jp/all?q=extraction","dc:title":"extraction"},{"@id":"https://cir.nii.ac.jp/all?q=filtering","dc:title":"filtering"}],"dcterms:subject":[{"subjectScheme":"Other","notation":[{"@language":"en","@value":"Biryu-chip"}]},{"subjectScheme":"Other","notation":[{"@language":"en","@value":"DNeasy extraction kit"}]},{"subjectScheme":"Other","notation":[{"@language":"en","@value":"eDNA"}]},{"subjectScheme":"Other","notation":[{"@language":"en","@value":"extraction"}]},{"subjectScheme":"Other","notation":[{"@language":"en","@value":"filtering"}]}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040287907243522176","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21H03649"},{"@type":"JGN","@value":"JP21H03649"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H03649/"}],"notation":[{"@language":"ja","@value":"堆積物コアDNAを用いた海跡湖の近過去魚類群集と環境変遷との関係解明"},{"@language":"en","@value":"Estimation of the relationship between fish communities and environmental transition in inland sea lakes using sediment core DNA"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050012570391595776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols"}]},{"@id":"https://cir.nii.ac.jp/crid/1050025031477836288","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Environmental DNA as a ‘Snapshot’ of Fish  Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1050294045369092864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines"}]},{"@id":"https://cir.nii.ac.jp/crid/1050303564712817792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Estimation of Fish Biomass Using Environmental DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1050856995323826688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Environmental DNA method for estimating salamander distribution in headwater streams, and a comparison of water sampling methods"}]},{"@id":"https://cir.nii.ac.jp/crid/1050870297870319104","@type":"Article","resourceType":"レビュー論文(review article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The detection of aquatic macroorganisms using environmental DNA analysis - 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