{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1050845762642397440.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"URI","@value":"http://hdl.handle.net/10130/4076"}},{"identifier":{"@type":"DOI","@value":"10.1371/journal.pone.0147114"}},{"identifier":{"@type":"URI","@value":"http://dx.plos.org/10.1371/journal.pone.0147114"}},{"identifier":{"@type":"PMID","@value":"26800339"}},{"identifier":{"@type":"HANDLE","@value":"10130/4076"}},{"identifier":{"@type":"NAID","@value":"120005853753"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea"}],"dc:language":"en","description":[{"notation":[{"@value":"Capnocytophaga ochracea is present in the dental plaque biofilm of patients with periodontitis. Biofilm cells change their phenotype through quorum sensing in response to fluctuations in cell-population density. Quorum sensing is mediated by auto-inducers (AIs). AI-2 is involved in intercellular signaling, and production of its distant precursor is catalyzed by LuxS, an enzyme involved in the activated methyl cycle. Our aim was to clarify the role of LuxS in biofilm formation by C. ochracea. Two luxS-deficient mutants, TmAI2 and LKT7, were constructed from C. ochracea ATCC 27872 by homologous recombination. The mutants produced significantly less AI-2 than the wild type. The growth rates of these mutants were similar to that of the wild-type in both undiluted Tryptic soy broth and 0.5 × Tryptic soy broth. However, according to crystal violet staining, they produced significantly less biofilm than the wild type. Confocal laser scanning microscopy and scanning electron microscopy showed that the biofilm of the TmAI2 strain had a rougher structure than that of the wild type. Complementation of TmAI-2 with extrinsic AI-2 from the culture supernatant of wild-type strain did not restore biofilm formation by the TmAI2 strain, but complementation of LKT7 strain with luxS partially restored biofilm formation. These results indicate that LuxS is involved in biofilm formation by C. ochracea, and that the attenuation of biofilm formation by the mutants is likely caused by a defect in the activated methyl cycle rather than by a loss of AI-2."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1070845762642397443","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000334747657"}],"foaf:name":[{"@value":"Hosohama-Saito, K"}]},{"@id":"https://cir.nii.ac.jp/crid/1070845762642397445","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000334747658"}],"foaf:name":[{"@value":"Kokubu, E"}]},{"@id":"https://cir.nii.ac.jp/crid/1070845762642397441","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000334747659"}],"foaf:name":[{"@value":"Okamoto-Shibayama, K"}]},{"@id":"https://cir.nii.ac.jp/crid/1070845762642397442","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000334747660"}],"foaf:name":[{"@value":"Kita, D"}]},{"@id":"https://cir.nii.ac.jp/crid/1070845762642397444","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000334747661"}],"foaf:name":[{"@value":"Katakura, A"}]},{"@id":"https://cir.nii.ac.jp/crid/1070845762642397440","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000334747662"}],"foaf:name":[{"@value":"Ishihara, K"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1380848662514953993","@type":"Researcher","foaf:name":[{"@value":"Jens Kreth"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"19326203"}],"prism:publicationName":[{"@value":"PloS one"}],"dc:publisher":[{"@value":"Public Library of Science"}],"prism:publicationDate":"2016-01-22","prism:volume":"11","prism:number":"1","prism:startingPage":"e0147114"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["©2016 Hosohama-Saito et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."],"url":[{"@id":"http://hdl.handle.net/10130/4076"},{"@id":"http://dx.plos.org/10.1371/journal.pone.0147114"}],"foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Confocal","dc:title":"Microscopy, Confocal"},{"@id":"https://cir.nii.ac.jp/all?q=Science","dc:title":"Science"},{"@id":"https://cir.nii.ac.jp/all?q=Q","dc:title":"Q"},{"@id":"https://cir.nii.ac.jp/all?q=R","dc:title":"R"},{"@id":"https://cir.nii.ac.jp/all?q=Carbon-Sulfur%20Lyases","dc:title":"Carbon-Sulfur Lyases"},{"@id":"https://cir.nii.ac.jp/all?q=Bacterial%20Proteins","dc:title":"Bacterial Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Biofilms","dc:title":"Biofilms"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Electron,%20Scanning","dc:title":"Microscopy, Electron, Scanning"},{"@id":"https://cir.nii.ac.jp/all?q=Medicine","dc:title":"Medicine"},{"@id":"https://cir.nii.ac.jp/all?q=Homologous%20Recombination","dc:title":"Homologous Recombination"},{"@id":"https://cir.nii.ac.jp/all?q=Capnocytophaga","dc:title":"Capnocytophaga"},{"@id":"https://cir.nii.ac.jp/all?q=Research%20Article","dc:title":"Research Article"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781853806336","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15K11023"},{"@type":"JGN","@value":"JP15K11023"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K11023/"}],"notation":[{"@language":"ja","@value":"根尖性歯周組織炎・口腔底蜂窩織炎病巣細菌の網羅的解析による病態-菌種間関係の解明"},{"@language":"en","@value":"Microbiome analysis of apical periodontitis and cellulitis in oral cavity"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050296298824795648","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"OxyR inactivation reduces the growth rate and oxidative stress defense in Capnocytophaga ochracea"}]},{"@id":"https://cir.nii.ac.jp/crid/1050568772251352448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A protein secretion system linked to bacteroidete gliding motility and pathogenesis."}]},{"@id":"https://cir.nii.ac.jp/crid/1050845762640737152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Susceptibility of Actinobacillus actinomycetemcomitans to six antibiotics decreases as biofilm 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