{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360002215826792320.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.biomaterials.2011.11.007"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0142961211013457?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0142961211013457?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"22118818"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"The differentiation and isolation of mouse embryonic stem cells toward hepatocytes using galactose-carrying substrata"}],"description":[{"notation":[{"@value":"A simple culture system to achieve the differentiation of embryonic stem (ES) cells toward hepatocytes with high efficiency is crucial in providing a cell source for the medical application. In this study, we report the effect of a matrix-dependent enrichment of ES cell-derived hepatocytes using immobilized poly(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) (PVLA) with E-cadherin-IgG Fc (E-cad-Fc) as a galactose-carrying substratum. PVLA and E-cad-Fc were confirmed to be stably co-adsorbed onto polystyrene surface by quartz crystal microbalance (QCM). We showed that the E-cad-Fc/PVLA hybrid substratum was efficient in culturing primary hepatocytes and maintaining liver functions, on which the undifferentiated ES cells also maintained high proliferative capability. Furthermore, ES cell-derived hepatocytes on this hybrid matrix expressed elevated level of liver specific genes and functions together with early expression of definitive hepatocyte marker, asialoglycoprotein receptor (ASGPR). Finally, we isolated a high percentage of cells (about 60%) with ASGPR expression after re-seeding onto PVLA-coated surface, and observed the elimination of the poorly differentiated cells (Gata6(+) and Sox17(+)) and the ones toward another cell lineage (brachyury(+) and Pdx1(+)). The system uses a glycopolymer as an extracellular substratum for isolation and enrichment of ES cell-derived hepatocytes with adequate homogeneity and functionality."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380003443996272899","@type":"Researcher","foaf:name":[{"@value":"Qingyuan Meng"}]},{"@id":"https://cir.nii.ac.jp/crid/1380003443996272897","@type":"Researcher","foaf:name":[{"@value":"Amranul Haque"}]},{"@id":"https://cir.nii.ac.jp/crid/1380003443996272896","@type":"Researcher","foaf:name":[{"@value":"Bayar Hexig"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658328579587","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30101207"},{"@type":"NRID","@value":"1000030101207"},{"@type":"NRID","@value":"9000411235696"}],"foaf:name":[{"@value":"Toshihiro Akaike"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01429612"}],"prism:publicationName":[{"@value":"Biomaterials"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2012-02","prism:volume":"33","prism:number":"5","prism:startingPage":"1414","prism:endingPage":"1427"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0142961211013457?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0142961211013457?httpAccept=text/plain"}],"createdAt":"2011-12-02","modifiedAt":"2024-04-15","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Vinyl%20Compounds","dc:title":"Vinyl Compounds"},{"@id":"https://cir.nii.ac.jp/all?q=Surface%20Properties","dc:title":"Surface Properties"},{"@id":"https://cir.nii.ac.jp/all?q=Endoderm","dc:title":"Endoderm"},{"@id":"https://cir.nii.ac.jp/all?q=Galactose","dc:title":"Galactose"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Differentiation","dc:title":"Cell Differentiation"},{"@id":"https://cir.nii.ac.jp/all?q=Asialoglycoprotein%20Receptor","dc:title":"Asialoglycoprotein Receptor"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Separation","dc:title":"Cell Separation"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Fc","dc:title":"Receptors, Fc"},{"@id":"https://cir.nii.ac.jp/all?q=Cadherins","dc:title":"Cadherins"},{"@id":"https://cir.nii.ac.jp/all?q=Disaccharides","dc:title":"Disaccharides"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Immobilized%20Proteins","dc:title":"Immobilized Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Phenotype","dc:title":"Phenotype"},{"@id":"https://cir.nii.ac.jp/all?q=Hepatocytes","dc:title":"Hepatocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Polystyrenes","dc:title":"Polystyrenes"},{"@id":"https://cir.nii.ac.jp/all?q=Adsorption","dc:title":"Adsorption"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, 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