{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360002216199768320.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.yexcr.2012.07.012"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0014482712003436?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0014482712003436?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"22841477"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Maintenance of mitochondrial genome distribution by mitochondrial AAA+ protein ClpX"}],"description":[{"notation":[{"@value":"The segregation of mitochondrial DNA (mtDNA) is important for the maintenance and transmission of the genome between generations. Recently, we clarified that human mitochondrial transcription factor A (TFAM) is required for equal distribution and symmetric segregation of mtDNA in cultured cells; however, the molecular mechanism involved is largely unknown. ClpX is an ATPase associated with various cellular activities (AAA+) proteins that localize to the mitochondrial matrix and is suggested to associate with mtDNA. In this study, we found that RNAi-mediated knockdown of ClpX in HeLa cells resulted in enlarged mtDNA nucleoids, which is very similar to that observed in TFAM-knockdown cells in several properties. The expression of TFAM protein was not significantly reduced in ClpX-knockdown cells. However, the enlarged mtDNA nucleoids caused by ClpX-knockdown were suppressed by overexpression of recombinant TFAM and the phenotype was not observed in knockdown with ClpP, a protease subunit of ClpXP. Endogenous ClpX and TFAM exist in close vicinity, and ClpX enhanced DNA-binding activity of TFAM in vitro. These results suggest that human ClpX, a novel mtDNA regulator, maintains mtDNA nucleoid distribution through TFAM function as a chaperone rather than as a protease and its involvement in mtDNA segregation."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801201683840","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80382844"},{"@type":"NRID","@value":"1000080382844"},{"@type":"NRID","@value":"9000017331349"},{"@type":"NRID","@value":"9000000188921"},{"@type":"NRID","@value":"9000006873014"},{"@type":"NRID","@value":"9000391969800"},{"@type":"NRID","@value":"9000241863337"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/kasashima-resmp"}],"foaf:name":[{"@value":"Katsumi Kasashima"}]},{"@id":"https://cir.nii.ac.jp/crid/1380284919356465153","@type":"Researcher","foaf:name":[{"@value":"Megumi Sumitani"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326210229504","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50221817"},{"@type":"NRID","@value":"1000050221817"},{"@type":"NRID","@value":"9000002029206"},{"@type":"NRID","@value":"9000017331352"},{"@type":"NRID","@value":"9000018833033"},{"@type":"NRID","@value":"9000011130169"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/EH000084"}],"foaf:name":[{"@value":"Hitoshi Endo"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00144827"}],"prism:publicationName":[{"@value":"Experimental Cell Research"}],"dc:publisher":[{"@value":"Elsevier 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Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Genome,%20Mitochondrial","dc:title":"Genome, Mitochondrial"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=RNA%20Interference","dc:title":"RNA Interference"},{"@id":"https://cir.nii.ac.jp/all?q=HeLa%20Cells","dc:title":"HeLa Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription%20Factors","dc:title":"Transcription Factors"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782123561216","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22590290"},{"@type":"JGN","@value":"JP22590290"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590290/"}],"notation":[{"@language":"ja","@value":"ミトコンドリアタンパク質と性ホルモンによる代謝制御機構の研究"},{"@language":"en","@value":"Study for metabolic regulatory mechanism by a mitochondrial protein and a sex 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