{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360002217848246272.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1105/tpc.113.113167"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1105/tpc.113.113167"}},{"identifier":{"@type":"PMID","@value":"24179131"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"<i>p</i>-Hydroxybenzoyl-Glucose Is a Zwitter Donor for the Biosynthesis of 7-Polyacylated Anthocyanin in Delphinium"}],"description":[{"notation":[{"@value":"The blue color of delphinium (Delphinium grandiflorum) flowers is produced by two 7-polyacylated anthocyanins, violdelphin and cyanodelphin. Violdelphin is derived from the chromophore delphinidin that has been modified at the 7-position by Glc and p-hydroxybenzoic acid (pHBA) molecules. Modification of violdelphin by linear conjugation of Glc and pHBA molecules to a Glc moiety at the 7-position produces cyanodelphin. We recently showed that anthocyanin 7-O-glucosylation in delphinium is catalyzed by the acyl-Glc-dependent anthocyanin glucosyltransferase (AAGT). Here, we sought to answer the question of which enzyme activities are necessary for catalyzing the transfer of Glc and pHBA moieties to 7-glucosylated anthocyanin. We found that these transfers were catalyzed by enzymes that use p-hydroxybenzoyl-Glc (pHBG) as a bifunctional acyl and glucosyl donor. In addition, we determined that violdelphin is synthesized via step-by-step enzymatic reactions catalyzed by two enzymes that use pHBG as an acyl or glucosyl donor. We also isolated a cDNA encoding a protein that has the potential for p-hydroxybenzoylation activity and two AAGT cDNAs that encode a protein capable of adding Glc to delphinidin 3-O-rutinoside-7-O-(6-O-[p-hydroxybenzoyl]-glucoside) to form violdelphin."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380847870997712896","@type":"Researcher","foaf:name":[{"@value":"Yuzo Nishizaki"}],"jpcoar:affiliationName":[{"@value":"Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870997712897","@type":"Researcher","foaf:name":[{"@value":"Motoki Yasunaga"}],"jpcoar:affiliationName":[{"@value":"Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870997712899","@type":"Researcher","foaf:name":[{"@value":"Emi 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Sasaki"}],"jpcoar:affiliationName":[{"@value":"Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10404651"},{"@type":"EISSN","@value":"1532298X"}],"prism:publicationName":[{"@value":"The Plant Cell"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2013-10","prism:volume":"25","prism:number":"10","prism:startingPage":"4150","prism:endingPage":"4165"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://aspb.org/publications/aspb-journals/open-articles"],"url":[{"@id":"https://syndication.highwire.org/content/doi/10.1105/tpc.113.113167"}],"createdAt":"2013-11-01","modifiedAt":"2021-02-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA,%20Complementary","dc:title":"DNA, Complementary"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Flowers","dc:title":"Flowers"},{"@id":"https://cir.nii.ac.jp/all?q=Delphinium","dc:title":"Delphinium"},{"@id":"https://cir.nii.ac.jp/all?q=Anthocyanins","dc:title":"Anthocyanins"},{"@id":"https://cir.nii.ac.jp/all?q=Glucose","dc:title":"Glucose"},{"@id":"https://cir.nii.ac.jp/all?q=Hydroxybenzoates","dc:title":"Hydroxybenzoates"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"},{"@id":"https://cir.nii.ac.jp/all?q=Acyltransferases","dc:title":"Acyltransferases"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Proteins","dc:title":"Plant Proteins"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257127958528","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23370016"},{"@type":"JGN","@value":"JP23370016"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370016/"}],"notation":[{"@language":"ja","@value":"液胞内における二次代謝化合物の糖修飾機構の解明"},{"@language":"en","@value":"Elucidation of modification mechanism of secondary metabolites in plant vacuoles."}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257414816896","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"13J08285"},{"@type":"JGN","@value":"JP13J08285"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13J08285/"}],"notation":[{"@language":"ja","@value":"デルフィニウムにおけるポリアシル化アントシアニン生合成遺伝子の全解明"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360025429430540800","@type":"Article","resourceType":"学術雑誌論文(journal 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