{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004230172775552.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/jemt.22819"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.22819"}},{"identifier":{"@type":"URI","@value":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/jemt.22819"}},{"identifier":{"@type":"PMID","@value":"28439996"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Energy dispersive spectroscopy‐scanning transmission electron microscope observations of free radical production in human polymorphonuclear leukocytes phagocytosing non‐opsonized <i>Tannerella forsythia</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>We investigated the association between human polymorphonuclear leukocytes (PMNs) and non‐opsonized <jats:italic>Tannerella forsythia</jats:italic> ATCC 43037 displaying a serum‐resistant surface layer (S‐layer). When PMNs were mixed with <jats:italic>T. forsythia</jats:italic> in suspension, the cells phagocytosed <jats:italic>T. forsythia</jats:italic> cells. Nitro blue tetrazolium (NBT) reduction, indicative of \n<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jemt22819-math-0001.png\" xlink:title=\"urn:x-wiley:1059910X:media:jemt22819:jemt22819-math-0001\"/> production, was observed by light microscopy; cerium (Ce) perhydroxide deposition, indicative of H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> production, was observed by electron microscopy. We examined the relationship between high‐molecular‐weight proteins of the S‐layer and Ce reaction (for <jats:italic>T. forsythia</jats:italic> phagocytosis) using electron microscopic immunolabeling. Immunogold particles were localized within the PMNs and on cell surfaces, labelling at the same Ce‐reacted sites where the S‐layer was present. We then used energy dispersive spectroscopy (EDS)‐scanning transmission electron microscope (STEM) to perform Ce and nitrogen (N) (for S‐layer immunocytochemistry) elemental analysis on the phagocytosed cells. That is, the elemental mapping and analysis of N by EDS appeared to reflect the presence of the same moieties detected by the 3,3′‐diaminobenzidine‐tetrahydrochloride (DAB) reaction with horseradish peroxidase (HRP)‐conjugated secondary antibodies, instead of immunogold labeling. We focused on the use of EDS‐STEM to visualize the presence of N resulting from the DAB reaction. In a parallel set of experiments, we used EDS‐STEM to perform Ce and gold (Au; from immunogold labeling of the S‐layer) elemental analysis on the same phagocytosing cells.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004230172775565","@type":"Researcher","foaf:name":[{"@value":"Keiichi Moriguchi"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Anatomy, School of Dentistry Aichi‐Gakuin University Nagoya Aichi 464‐8650 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276166337152","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70460524"},{"@type":"NRID","@value":"1000070460524"},{"@type":"NRID","@value":"9000283735318"},{"@type":"NRID","@value":"9000392732263"},{"@type":"NRID","@value":"9000004730329"},{"@type":"NRID","@value":"9000415181533"},{"@type":"NRID","@value":"9000258692373"},{"@type":"NRID","@value":"9000411534463"},{"@type":"NRID","@value":"9000336052261"},{"@type":"NRID","@value":"9000411367280"},{"@type":"NRID","@value":"9000019178752"},{"@type":"NRID","@value":"9000006540812"},{"@type":"NRID","@value":"9000402066371"},{"@type":"NRID","@value":"9000252981420"},{"@type":"NRID","@value":"9000401694626"},{"@type":"NRID","@value":"9000401630612"}],"foaf:name":[{"@value":"Yoshiaki Hasegawa"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology, School of Dentistry Aichi‐Gakuin University Nagoya Aichi 464‐8650 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004230172775432","@type":"Researcher","foaf:name":[{"@value":"Naoya Higuchi"}],"jpcoar:affiliationName":[{"@value":"Department of Endodontics, School of Dentistry Aichi‐Gakuin University Nagoya Aichi 464‐8650 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Murakami"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Microbiology, Division of Oral Infections and Health Science Asahi University School of Dentistry 1851 Hozumi Mizuho Gifu 501‐0296 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004230172775297","@type":"Researcher","foaf:name":[{"@value":"Fuminobu Yoshimura"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology, School of Dentistry Aichi‐Gakuin University Nagoya Aichi 464‐8650 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004230172775303","@type":"Researcher","foaf:name":[{"@value":"Kazuhiko Nakata"}],"jpcoar:affiliationName":[{"@value":"Department of Endodontics, School of Dentistry Aichi‐Gakuin University Nagoya Aichi 464‐8650 Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004230172775436","@type":"Researcher","foaf:name":[{"@value":"Masaki Honda"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Anatomy, School of Dentistry Aichi‐Gakuin University Nagoya Aichi 464‐8650 Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"1059910X"},{"@type":"EISSN","@value":"10970029"}],"prism:publicationName":[{"@value":"Microscopy Research and Technique"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2017-04-24","prism:volume":"80","prism:number":"6","prism:startingPage":"555","prism:endingPage":"562"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.22819"},{"@id":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/jemt.22819"}],"createdAt":"2017-04-25","modifiedAt":"2025-10-16","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Neutrophils","dc:title":"Neutrophils"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Membrane","dc:title":"Cell Membrane"},{"@id":"https://cir.nii.ac.jp/all?q=Spectrometry,%20X-Ray%20Emission","dc:title":"Spectrometry, X-Ray Emission"},{"@id":"https://cir.nii.ac.jp/all?q=Cerium","dc:title":"Cerium"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrogen%20Peroxide","dc:title":"Hydrogen Peroxide"},{"@id":"https://cir.nii.ac.jp/all?q=Immunohistochemistry","dc:title":"Immunohistochemistry"},{"@id":"https://cir.nii.ac.jp/all?q=Oxygen","dc:title":"Oxygen"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Electron,%20Transmission","dc:title":"Microscopy, Electron, Transmission"},{"@id":"https://cir.nii.ac.jp/all?q=Phagocytosis","dc:title":"Phagocytosis"},{"@id":"https://cir.nii.ac.jp/all?q=Tannerella%20forsythia","dc:title":"Tannerella forsythia"},{"@id":"https://cir.nii.ac.jp/all?q=Hydroxides","dc:title":"Hydroxides"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Periodontitis","dc:title":"Periodontitis"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781917462400","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16K11464"},{"@type":"JGN","@value":"JP16K11464"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K11464/"}],"notation":[{"@language":"ja","@value":"歯周病関連細菌の外膜蛋白質の糖鎖修飾に関与する酵素の探索と機能の解明"},{"@language":"en","@value":"Search and characterization of glycosylation-related enzymes of outer membrane proteins from periodantal 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