{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004232117368448.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.ejphar.2017.12.042"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0014299917308361?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0014299917308361?httpAccept=text/plain"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"The natural flavonoid myricetin inhibits gastric H + , K + -ATPase"}],"description":[{"notation":[{"@value":"Myricetin (3,3',4',5,5',7-hexahydroxyflavone), a major flavonoid in berries and red wine, has been recently used as a health food supplement based on its antioxidant and antitumor properties. We report here that myricetin preferentially exerts inhibitory effects on gastric H+, K+-ATPase. Myricetin inhibited H+, K+-ATPase with a sub-micromolar IC50 value in an enzyme assay using freeze-dried tubulovesicles prepared from hog stomach. Na+, K+-ATPase and Ca2+-ATPase were also inhibited by myricetin in a dose-dependent manner, but the IC50 values for these enzymes were approximately an order of magnitude higher compared to the H+, K+-ATPase. In structure-inhibitory functional analysis of sixteen myricetin derivatives, several phenolic hydroxy groups attached to the flavonoid backbone were highlighted as essential modifications for the inhibition of P2-type ATPases. Furthermore, oral administration of myricetin significantly attenuated histamine-induced gastric acid secretion in an in vivo mouse assessment. Therefore, myricetin derivatives seem to be useful seed compounds for developing new drugs and supplements to alleviate gastric acid secretion."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004232117368449","@type":"Researcher","foaf:name":[{"@value":"Yuu Miyazaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276170892416","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10609209"},{"@type":"NRID","@value":"1000010609209"},{"@type":"ORCID","@value":"0000-0003-0366-5211"},{"@type":"NRID","@value":"9000411833113"},{"@type":"NRID","@value":"9000345289578"},{"@type":"NRID","@value":"9000413572907"},{"@type":"NRID","@value":"9000018534016"},{"@type":"NRID","@value":"9000002129929"},{"@type":"NRID","@value":"9000021815029"},{"@type":"NRID","@value":"9000240064239"},{"@type":"NRID","@value":"9000309185993"},{"@type":"NRID","@value":"9000410919371"},{"@type":"NRID","@value":"9000391612812"},{"@type":"NRID","@value":"9000281369061"},{"@type":"NRID","@value":"9000283540430"},{"@type":"NRID","@value":"9000023792389"},{"@type":"NRID","@value":"9000341541593"},{"@type":"NRID","@value":"9000241187289"},{"@type":"NRID","@value":"9000410157740"},{"@type":"NRID","@value":"9000408480306"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/7000011411"}],"foaf:name":[{"@value":"Atsuhiko Ichimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232117368452","@type":"Researcher","foaf:name":[{"@value":"Shun Sato"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326210760960","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50567980"},{"@type":"NRID","@value":"1000050567980"},{"@type":"NRID","@value":"9000404317411"},{"@type":"NRID","@value":"9000019991915"},{"@type":"NRID","@value":"9000402822809"},{"@type":"NRID","@value":"9000408485995"},{"@type":"NRID","@value":"9000017289798"},{"@type":"NRID","@value":"9000398138048"},{"@type":"NRID","@value":"9000398292433"},{"@type":"NRID","@value":"9000408487622"}],"foaf:name":[{"@value":"Takuto Fujii"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232117368454","@type":"Researcher","foaf:name":[{"@value":"Shinya Oishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232117368450","@type":"Researcher","foaf:name":[{"@value":"Hideki Sakai"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232117368448","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Takeshima"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00142999"}],"prism:publicationName":[{"@value":"European Journal of Pharmacology"}],"dc:publisher":[{"@value":"Elsevier 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