{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004232370201088.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.mex.2016.06.001"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S2215016116300255?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S2215016116300255?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"27489781"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"A genetically encoded bioluminescent indicator for illuminating proinflammatory cytokines"}],"description":[{"notation":[{"@value":"We introduce a method to evaluate the activities of cytokines based on the nuclear transport of NF-κB. A pair of bioluminescent indicators was made for conferring cytokine sensitivity to cervical carcinoma-derived HeLa cells. The principle is based on reconstitution of split fragments of Renilla reniformis luciferase (RLuc) by protein splicing with a DnaE intein from Synechocystis sp. PCC6803. The bioluminescence intensity of thus reconstituted RLuc in the HeLa cells was used as a measure of the activities for cytokines. With the present method, we evaluated the activities of various cytokines based on the nuclear transport of NF-κB in human cervical carcinoma-derived HeLa cells carrying the indicators. The present approach to evaluating the activities of cytokines may provide a potential clinical value in monitoring drug activity and directing treatment for various diseases related with NF-κB. The method highlights the experimental procedure from our original publications, Anal. Biochem. 2006, 359, 147-149 and Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 11542. The summary of the method is: •Cytokine activities are determined within 2 h after stimulation.•Temporarily inactivated split-luciferase fragments are reconstituted by protein splicing.•Nucleartrafficking of NF-κB was illuminated for gauging the ligand-driven activity."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004232370201088","@type":"Researcher","foaf:name":[{"@value":"Sung Bae Kim"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232370201090","@type":"Researcher","foaf:name":[{"@value":"Takeaki Ozawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232370201089","@type":"Researcher","foaf:name":[{"@value":"Yoshio Umezawa"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"22150161"}],"prism:publicationName":[{"@value":"MethodsX"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2016","prism:volume":"3","prism:startingPage":"483","prism:endingPage":"489"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S2215016116300255?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S2215016116300255?httpAccept=text/plain"}],"createdAt":"2016-07-08","modifiedAt":"2019-08-09","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Chemistry","dc:title":"Chemistry"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782286833024","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"26288088"},{"@type":"JGN","@value":"JP26288088"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26288088/"}],"notation":[{"@language":"ja","@value":"発光性細胞株アレイを用いた高速PM2.5評価系の構築"},{"@language":"en","@value":"Development of a high throughput bioassay system for PM2.5 with luminescent cell arrays"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256897067392","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16K14051"},{"@type":"JGN","@value":"JP16K14051"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14051/"}],"notation":[{"@language":"ja","@value":"再生水中の生理活性物質による潜在的健康リスクの発色評価"},{"@language":"en","@value":"Determination of potential health risks of bioactive chemicals in reclaimed water"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011145583563392","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Labor-effective manipulation of marine and beetle luciferases for bioassays"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292618951616512","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Genetically Encoded Bioluminescent Indicator for ERK2 Dimer in Living Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621195834240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096435831296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"NF-&#954;B in Human Disease: Current Inhibitors and Prospects for De Novo Structure Based Design of Inhibitors"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569386584960","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Anti-TNF-α therapies: the next generation"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896310753536","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Real-time imaging of ligand-induced IKK activation in intact cells and in living mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370581167104","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Monitoring Protein−Protein Interactions Using Split Synthetic Renilla Luciferase Protein-Fragment-Assisted Complementation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107371188187008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A proinflammatory cytokine sensor cell for assaying inflammatory activities of nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320174497536","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A method for determining the activities of cytokines based on the nuclear transport of nuclear factor-κB"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320587586048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A simple method for displaying the hydropathic character of a protein"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794745558144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split\n                    <i>Renilla</i>\n                    luciferase"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269882003584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Diverse agents act at multiple levels to inhibit the Rel/NF-κB signal transduction pathway"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.mex.2016.06.001"},{"@type":"KAKEN","@value":"PRODUCT-21020899"},{"@type":"KAKEN","@value":"PRODUCT-21277140"},{"@type":"OPENAIRE","@value":"doi_dedup___::998995fb0a8cf092f7839e33647a63b5"}]}