{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004232548428800.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.vascn.2015.06.001"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S1056871915000702?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S1056871915000702?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"26070404"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Construction of sensitive reporter assay yeasts for comprehensive detection of ligand activities of human corticosteroid receptors through inactivation of CWP and PDR genes"}],"description":[{"notation":[{"@value":"The glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) are members of the nuclear receptor superfamily and ligand-dependent transcription factors, whose major ligands are glucocorticoid and mineralocorticoid, so-called corticosteroids. The corticosteroids are a class of substances that include steroid hormones naturally produced in the adrenal cortex of vertebrates and analogues of these hormones that are synthesized in industry. They are involved in a wide range of physiological processes including stress and immune responses, and the regulation of carbohydrate metabolism, protein catabolism, sodium homeostasis, and inflammation. These substances are potential environmental contaminants because they are clinically consumed in large amounts worldwide. To develop a simple and sensitive bioassay to detect corticosteroids, we newly established reporter assay yeasts expressing human GR and MR.Ligand responses of the established assay yeasts were improved by forced expression of a human transcription coactivator SRC-1e. Further enhancement of the responses was achieved by inactivating the CWP and PDR genes that encode cell wall mannoproteins and plasma membrane efflux pumps, respectively, which may be attributable to an increased intracellular concentration of ligands.These new assay yeasts were more responsive to both natural and synthetic agonist ligands than the conventional assay yeasts. They detected both agonistic and antagonistic activities of mifepristone, spironolactone, and eplerenone in a receptor-selective manner. They also detected ligand activities contained in oral pharmaceutical tablets and human urine.This assay system will be a valuable tool to detect agonists as well as antagonists of corticosteroid receptors, in the fields of drug discovery and the assessment of environmental pollutants."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004232548428800","@type":"Researcher","foaf:name":[{"@value":"Sayoko Ito-Harashima"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276182879488","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20391112"},{"@type":"NRID","@value":"1000020391112"},{"@type":"NRID","@value":"9000014611519"},{"@type":"NRID","@value":"9000006775763"},{"@type":"NRID","@value":"9000257967238"},{"@type":"NRID","@value":"9000317127742"},{"@type":"NRID","@value":"9000006952483"},{"@type":"NRID","@value":"9000317128042"},{"@type":"NRID","@value":"9000317127280"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0130144"}],"foaf:name":[{"@value":"Kazuhiro Shiizaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232548428806","@type":"Researcher","foaf:name":[{"@value":"Masanobu Kawanishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232548428801","@type":"Researcher","foaf:name":[{"@value":"Koji Kakiuchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232548428803","@type":"Researcher","foaf:name":[{"@value":"Kana Onishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232548428802","@type":"Researcher","foaf:name":[{"@value":"Ryoichi Yamaji"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004232548428804","@type":"Researcher","foaf:name":[{"@value":"Takashi Yagi"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10568719"}],"prism:publicationName":[{"@value":"Journal of Pharmacological and Toxicological Methods"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2015-07","prism:volume":"74","prism:startingPage":"41","prism:endingPage":"52"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","http://www.elsevier.com/open-access/userlicense/1.0/","https://doi.org/10.15223/policy-017","https://doi.org/10.15223/policy-037","https://doi.org/10.15223/policy-012","https://doi.org/10.15223/policy-029","https://doi.org/10.15223/policy-004"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S1056871915000702?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S1056871915000702?httpAccept=text/plain"}],"createdAt":"2015-06-12","modifiedAt":"2025-05-28","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Steroid","dc:title":"Receptors, Steroid"},{"@id":"https://cir.nii.ac.jp/all?q=Spironolactone","dc:title":"Spironolactone"},{"@id":"https://cir.nii.ac.jp/all?q=Ligands","dc:title":"Ligands"},{"@id":"https://cir.nii.ac.jp/all?q=Eplerenone","dc:title":"Eplerenone"},{"@id":"https://cir.nii.ac.jp/all?q=Mifepristone","dc:title":"Mifepristone"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Glucocorticoid","dc:title":"Receptors, Glucocorticoid"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Mineralocorticoid","dc:title":"Receptors, Mineralocorticoid"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20Reporter","dc:title":"Genes, Reporter"},{"@id":"https://cir.nii.ac.jp/all?q=Yeasts","dc:title":"Yeasts"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Biological%20Assay","dc:title":"Biological Assay"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282256823836416","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15K06849"},{"@type":"JGN","@value":"JP15K06849"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K06849/"}],"notation":[{"@language":"ja","@value":"ゲノムDNA上に生じた一分子の付加体の測定方法の開発"},{"@language":"en","@value":"Development of a method to identify single molecule adducts generated on genomic DNA"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335849297024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"New reporter gene assays for detecting natural and synthetic molting hormone agonists using yeasts expressing ecdysone receptors of various insects"}]},{"@id":"https://cir.nii.ac.jp/crid/1050023239833531776","@type":"Article","resourceType":"学術雑誌論文(journal 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