{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004233127260928.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/acs.jafc.6b04521"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.6b04521"}},{"identifier":{"@type":"PMID","@value":"27977180"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"A Limonoid Kihadanin B from Immature <i>Citrus unshiu</i> Peels Suppresses Adipogenesis through Repression of the Akt-FOXO1-PPARγ Axis in Adipocytes"}],"description":[{"notation":[{"@value":"Citrus limonoids are secondary metabolites and exhibit a variety of biological activities. In this study, we elucidated the suppression of adipogenesis by a Citrus limonoid kihadanin B and determined its molecular mechanism in mouse 3T3-L1 adipocytes. Kihadanin B was purified from the peels of immature Citrus unshiu by HPLC, and its chemical structure was determined by NMR and mass spectrometry. Kihadanin B reduced the lipid accumulation with the reduction of the expression levels of the adipogenic and lipogenic genes, but did not affect lipolysis in adipocytes. Phosphorylation levels of Akt and a forkhead transcriptional factor, FOXO1, a repressor of PPARγ, were lowered by kihadanin B. Furthermore, kihadanin B increased the binding level of FOXO1 to the PPARγ gene promoter in adipocytes. These results indicate that a Citrus limonoid kihadanin B repressed the adipogenesis by decreasing lipid accumulation through the suppression of the Akt-FOXO1-PPARγ axis in 3T3-L1 adipocytes."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004233127260802","@type":"Researcher","foaf:name":[{"@value":"Shizuka Baba"}],"jpcoar:affiliationName":[{"@value":"Laboratory\rof Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233127260928","@type":"Researcher","foaf:name":[{"@value":"Yasuaki Ueno"}],"jpcoar:affiliationName":[{"@value":"Laboratory\rof Medicinal Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233127260801","@type":"Researcher","foaf:name":[{"@value":"Takashi Kikuchi"}],"jpcoar:affiliationName":[{"@value":"Laboratory\rof Medicinal Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233127260929","@type":"Researcher","foaf:name":[{"@value":"Reiko Tanaka"}],"jpcoar:affiliationName":[{"@value":"Laboratory\rof Medicinal Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801189541248","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70425453"},{"@type":"NRID","@value":"1000070425453"},{"@type":"NRID","@value":"9000004408848"},{"@type":"NRID","@value":"9000259831701"},{"@type":"NRID","@value":"9000324995853"},{"@type":"NRID","@value":"9000011268205"},{"@type":"NRID","@value":"9000004259216"},{"@type":"NRID","@value":"9000283404672"},{"@type":"NRID","@value":"9000019140084"},{"@type":"NRID","@value":"9000413473562"},{"@type":"NRID","@value":"9000409490176"},{"@type":"NRID","@value":"9000258744186"},{"@type":"NRID","@value":"9000021412827"},{"@type":"NRID","@value":"9000303996947"},{"@type":"NRID","@value":"9000253182147"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/fujimorik"}],"foaf:name":[{"@value":"Ko Fujimori"}],"jpcoar:affiliationName":[{"@value":"Laboratory\rof Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00218561"},{"@type":"EISSN","@value":"15205118"}],"prism:publicationName":[{"@value":"Journal of Agricultural and Food Chemistry"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2016-12-15","prism:volume":"64","prism:number":"51","prism:startingPage":"9607","prism:endingPage":"9615"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.6b04521"}],"createdAt":"2016-12-05","modifiedAt":"2023-04-15","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Limonins","dc:title":"Limonins"},{"@id":"https://cir.nii.ac.jp/all?q=Citrus","dc:title":"Citrus"},{"@id":"https://cir.nii.ac.jp/all?q=Adipogenesis","dc:title":"Adipogenesis"},{"@id":"https://cir.nii.ac.jp/all?q=Forkhead%20Box%20Protein%20O1","dc:title":"Forkhead Box Protein O1"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Extracts","dc:title":"Plant Extracts"},{"@id":"https://cir.nii.ac.jp/all?q=PPAR%20gamma","dc:title":"PPAR gamma"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=3T3-L1%20Cells","dc:title":"3T3-L1 Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Fruit","dc:title":"Fruit"},{"@id":"https://cir.nii.ac.jp/all?q=Adipocytes","dc:title":"Adipocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Proto-Oncogene%20Proteins%20c-akt","dc:title":"Proto-Oncogene Proteins c-akt"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781913865344","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16K08256"},{"@type":"JGN","@value":"JP16K08256"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08256/"}],"notation":[{"@language":"ja","@value":"エイコサノイドによる肥満制御機構の解明とその制御機構に基づく新規治療法の探索"},{"@language":"en","@value":"Regulation mechanism of obesity by eicosanoids and development of therapeutic 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