{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004233240784768.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/la400059h"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/la400059h"}},{"identifier":{"@type":"PMID","@value":"23590586"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Molecular Assembly of Zinc Chlorophyll Derivatives by Using Recombinant Light-Harvesting Polypeptides with His-tag and Immobilization on a Gold Electrode"}],"description":[{"notation":[{"@value":"LH1-α and -β polypeptides, which make up the light-harvesting 1 (LH1) complex of purple photosynthetic bacteria, along with bacteriochlorophylls, have unique binding properties even for various porphyrin analogs. Herein, we used the porphyrin analogs, Zn-Chlorin and the Zn-Chlorin dimer, and examined their binding behaviors to the LH1-α variant, which has a His-tag at the C-terminus (MBP-rubα-YH). Zn-Chlorin and the Zn-Chlorin dimer could bind to MBP-rubα-YH and form a subunit-type assembly, similar to that from the native LH1 complex. These complexes could be immobilized onto Ni-nitrilotriacetic acid-modified Au electrodes, and the cathodic photocurrent was successfully observed by photoirradiation. Since Zn-Chlorins in this complex are too far for direct electron transfer from the electrode, a contribution of polypeptide backbone for efficient electron transfer was implied. These findings not only show interesting properties of LH1-α polypeptides but also suggest a clue to construct artificial photosynthesis systems using these peptide materials."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004233240784773","@type":"Researcher","foaf:name":[{"@value":"Shunsuke Sakai"}],"jpcoar:affiliationName":[{"@value":"Graduate School\rof Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku,\rNagoya 466-8555, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784772","@type":"Researcher","foaf:name":[{"@value":"Tomoyasu Noji"}],"jpcoar:affiliationName":[{"@value":"Graduate School\rof Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku,\rNagoya 466-8555, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784770","@type":"Researcher","foaf:name":[{"@value":"Masaharu Kondo"}],"jpcoar:affiliationName":[{"@value":"Graduate School\rof Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku,\rNagoya 466-8555, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784774","@type":"Researcher","foaf:name":[{"@value":"Toshihisa Mizuno"}],"jpcoar:affiliationName":[{"@value":"Graduate School\rof Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku,\rNagoya 466-8555, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751153885952","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70335082"},{"@type":"NRID","@value":"1000070335082"},{"@type":"NRID","@value":"9000258718407"},{"@type":"NRID","@value":"9000257783959"},{"@type":"NRID","@value":"9000256019865"},{"@type":"NRID","@value":"9000000824797"},{"@type":"NRID","@value":"9000018807908"},{"@type":"NRID","@value":"9000240539270"},{"@type":"NRID","@value":"9000403598091"},{"@type":"NRID","@value":"9000257723348"},{"@type":"NRID","@value":"9000000305897"},{"@type":"NRID","@value":"9000257734677"},{"@type":"NRID","@value":"9000283245604"},{"@type":"NRID","@value":"9000257715134"},{"@type":"NRID","@value":"9000257733408"},{"@type":"NRID","@value":"9000402791723"},{"@type":"NRID","@value":"9000002741156"},{"@type":"NRID","@value":"9000259336344"},{"@type":"NRID","@value":"9000259289390"},{"@type":"NRID","@value":"9000240037552"},{"@type":"NRID","@value":"9000242734684"},{"@type":"NRID","@value":"9000403597686"},{"@type":"NRID","@value":"9000367420322"},{"@type":"NRID","@value":"9000257728108"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0100881"}],"foaf:name":[{"@value":"Takehisa Dewa"}],"jpcoar:affiliationName":[{"@value":"Graduate School\rof Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku,\rNagoya 466-8555, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784768","@type":"Researcher","foaf:name":[{"@value":"Tsuyoshi Ochiai"}],"jpcoar:affiliationName":[{"@value":"Kanagawa Academy of Science and Technology, 3-2-1 Sakado, Takatsu-ku,\rKawasaki, Kanagawa 213-0012, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784769","@type":"Researcher","foaf:name":[{"@value":"Hisanori Yamakawa"}],"jpcoar:affiliationName":[{"@value":"Division of Material Science (Physics),\rGraduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464−8602, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784775","@type":"Researcher","foaf:name":[{"@value":"Shigeru Itoh"}],"jpcoar:affiliationName":[{"@value":"Division of Material Science (Physics),\rGraduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464−8602, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784771","@type":"Researcher","foaf:name":[{"@value":"Hideki Hashimoto"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku,\rOsaka 558-8585, Japan"},{"@value":"Osaka\rCity University Advanced\rResearch Institute for Natural Science and Technology, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku,\rOsaka 558-8585, Japan"},{"@value":"CREST, Japan\rScience and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama\r332-0012, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233240784776","@type":"Researcher","foaf:name":[{"@value":"Mamoru Nango"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku,\rOsaka 558-8585, Japan"},{"@value":"Osaka\rCity University Advanced\rResearch Institute for Natural Science and Technology, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku,\rOsaka 558-8585, Japan"},{"@value":"CREST, Japan\rScience and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama\r332-0012, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"07437463"},{"@type":"EISSN","@value":"15205827"}],"prism:publicationName":[{"@value":"Langmuir"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2013-04-19","prism:volume":"29","prism:number":"17","prism:startingPage":"5104","prism:endingPage":"5109"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/la400059h"}],"createdAt":"2013-04-16","modifiedAt":"2023-04-07","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Chlorophyll","dc:title":"Chlorophyll"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Structure","dc:title":"Molecular Structure"},{"@id":"https://cir.nii.ac.jp/all?q=Light-Harvesting%20Protein%20Complexes","dc:title":"Light-Harvesting Protein Complexes"},{"@id":"https://cir.nii.ac.jp/all?q=Recombinant%20Proteins","dc:title":"Recombinant Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Zinc","dc:title":"Zinc"},{"@id":"https://cir.nii.ac.jp/all?q=Immobilized%20Proteins","dc:title":"Immobilized Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Histidine","dc:title":"Histidine"},{"@id":"https://cir.nii.ac.jp/all?q=Gold","dc:title":"Gold"},{"@id":"https://cir.nii.ac.jp/all?q=Peptides","dc:title":"Peptides"},{"@id":"https://cir.nii.ac.jp/all?q=Electrodes","dc:title":"Electrodes"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782174553216","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23655196"},{"@type":"JGN","@value":"JP23655196"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655196/"}],"notation":[{"@language":"ja","@value":"シリカナノ細孔内の「天然―人工ハイブリッド光合成系」の構築"},{"@language":"en","@value":"Construction of natural-artificical hybrid photosynthetic systems  inside  silica nanopores"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257166406528","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"24107002"},{"@type":"JGN","@value":"JP24107002"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-24107002/"}],"notation":[{"@language":"ja","@value":"光捕集機能を有する人工光合成システム"},{"@language":"en","@value":"Artificial photosynthetic systems with light-harvesting ability"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257197304064","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"24655120"},{"@type":"JGN","@value":"JP24655120"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24655120/"}],"notation":[{"@language":"ja","@value":"人工チラコイド膜の構築と機能評価"},{"@language":"en","@value":"Construction and Functional Analysis of Artificial Thylakoid Membranes"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257414559488","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"13J07029"},{"@type":"JGN","@value":"JP13J07029"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13J07029/"}],"notation":[{"@language":"ja","@value":"人工光合成を目指したタンパク質設計とその色素複合体の電極基盤上への組織化"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002219109801344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Structural Requirements of Zinc Porphyrin Derivatives on the Complex-Forming with Light-Harvesting Polypeptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231429652224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Light-energy conversion systems for hydrogen production and photocurrent generation using zinc chlorin derivatives"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145507789824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"High-Level Expression of Soluble Protein inEscherichia coliUsing a His6-Tag and Maltose-Binding-Protein Double-Affinity Fusion System"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706992030976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Synthesis of chlorophyll–amino acid conjugates as models for modification of proteins with chromo/fluorophores"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182150619264","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Photoinduced hydrogen production with photosensitization of Zn chlorophyll analog dimer as a photosynthetic special pair model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182481013760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Self-aggregation of a synthetic zinc chlorophyll derivative possessing a 131-dicyanomethylene group as a light-harvesting antenna model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094132408704","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis."}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095513581312","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Immobilization of Porphyrin Derivatives with a Defined Distance and Orientation onto a Gold Electrode Using Synthetic Light-Harvesting α-Helix Hydrophobic Polypeptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848657455206784","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Synthesis of 3,20-disubstituted chlorophyll-a derivatives and reactivity of the substituents"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660744345216","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Self‐aggregation of Synthetic Zinc Chlorophyll Derivatives Possessing 3<sup>1</sup>‐Hydroxy or Methoxy Group and 13<sup>1</sup>‐Mono‐ or Dicyanomethylene Moiety in Nonpolar Organic Solvents as Models of Chlorosomal Bacteriochlorophyll‐<i>d</i> Aggregates"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044299213440","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Long-Range Energy Propagation in Nanometer Arrays of Light Harvesting Antenna Complexes"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520105603968","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Incorporation of Glucose Oxidase into Langmuir−Blodgett Films Based on Prussian Blue Applied to Amperometric Glucose Biosensor"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521255274112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"NADH oxidation using modified electrodes based on lactate and glucose dehydrogenase entrapped between an electrocatalyst film and redox catalyst-modified polymers"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993524053248","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Immobilization of Light-Harvesting Chlorophyll <i>a</i>/<i>b</i> Complex (LHCIIb) Studied by Surface Plasmon Field-Enhanced Fluorescence Spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994896715776","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Reconstitution of the B873 light-harvesting complex of Rhodospirillum rubrum from the separately isolated .alpha.- and .beta.-polypeptides and bacteriochlorophyll a"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996339857280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Molecular Photodiode System That Can Switch Photocurrent Direction"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944722681600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Orientated binding of photosynthetic reaction centers on gold using NiNTA self-assembled monolayers"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944841650560","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparison of the Structural Requirements for Bacteriochlorophyll Binding in the Core Light-Harvesting Complexes of <i>Rhodospirillum rubrum</i> and <i>Rhodobacter sphaeroides</i> Using Reconstitution Methodology with Bacteriochlorophyll Analogs"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419521580800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Solution properties of synthetic chlorophyllide- and bacteriochlorophyllide-apomyoglobin complexes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844752362880","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Photocurrent Generation under a Large Dipole Moment Formed by Self-Assembled Monolayers of Helical Peptides Having an <i>N</i>-Ethylcarbazolyl Group"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318353347584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Use of Engineered Unique Cysteine Residues to Facilitate Oriented Coupling of Proteins Directly to a Gold Substrate"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268723526144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biohybrid Photosynthetic Antenna Complexes for Enhanced Light-Harvesting"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204588029184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Reconstitution and Organization of Photosynthetic Antenna Protein Complex Bearing Functional Hydrophilic Domains"},{"@value":"Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1021/la400059h"},{"@type":"KAKEN","@value":"PRODUCT-14250585"},{"@type":"KAKEN","@value":"PRODUCT-14456999"},{"@type":"KAKEN","@value":"PRODUCT-14761437"},{"@type":"KAKEN","@value":"PRODUCT-9512225"},{"@type":"OPENAIRE","@value":"doi_dedup___::99535b47d505b4d3bd8ba7fc00c481c8"},{"@type":"CROSSREF","@value":"10.1007/s11164-016-2659-8_references_DOI_QhwH2aMPZH62n00NOi5LhAyaUO5"},{"@type":"CROSSREF","@value":"10.1016/j.tet.2013.07.060_references_DOI_QhwH2aMPZH62n00NOi5LhAyaUO5"},{"@type":"CROSSREF","@value":"10.1016/j.tetlet.2014.03.071_references_DOI_QhwH2aMPZH62n00NOi5LhAyaUO5"},{"@type":"CROSSREF","@value":"10.1111/php.12327_references_DOI_QhwH2aMPZH62n00NOi5LhAyaUO5"},{"@type":"CROSSREF","@value":"10.1016/j.ijhydene.2015.01.158_references_DOI_QhwH2aMPZH62n00NOi5LhAyaUO5"},{"@type":"CROSSREF","@value":"10.1016/j.bmc.2013.12.059_references_DOI_QhwH2aMPZH62n00NOi5LhAyaUO5"}]}