{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004233482890624.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41598-017-17112-8"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41598-017-17112-8.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41598-017-17112-8"}},{"identifier":{"@type":"PMID","@value":"29196648"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Sequestosome1/p62 protects mouse embryonic fibroblasts against low-dose methylercury-induced cytotoxicity and is involved in clearance of ubiquitinated proteins"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Methylmercury (MeHg) is a widely distributed environmental pollutant that causes a series of cytotoxic effects. However, molecular mechanisms underlying MeHg toxicity are not fully understood. Here, we report that sequestosome1/p62 protects mouse embryonic fibroblasts (MEFs) against low-dose MeHg cytotoxicity via clearance of MeHg-induced ubiquitinated proteins. p62 mRNA and protein expression in MEFs were temporally induced by MeHg exposure p62-deficient MEFs exhibited higher sensitivity to MeHg exposure compared to their wild-type (WT) counterparts. An earlier and higher level of accumulation of ubiquitinated proteins was detected in p62-deficient cells compared with WT MEFs. Confocal microscopy revealed that p62 and ubiquitinated proteins co-localized in the perinuclear region of MEFs following MeHg treatment. Further analysis of MEFs revealed that ubiquitinated proteins co-localized with LC3-positive puncta upon co-treatment with MeHg and chloroquine, an autophagy inhibitor. In contrast, there was minimal co-localization in p62-deficient MEFs. The present study, for the first time, examined the expression and distribution of p62 and ubiquitinated proteins in cells exposed to low-dose MeHg. Our findings suggest that p62 is crucial for cytoprotection against MeHg-induced toxicity and is required for MeHg-induced ubiquitinated protein clearance.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276179422080","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90345257"},{"@type":"NRID","@value":"1000090345257"},{"@type":"NRID","@value":"9000396173480"},{"@type":"NRID","@value":"9000345261020"},{"@type":"NRID","@value":"9000386309493"},{"@type":"NRID","@value":"9000403275299"},{"@type":"NRID","@value":"9000375908499"},{"@type":"NRID","@value":"9000399355042"},{"@type":"NRID","@value":"9000391550809"},{"@type":"NRID","@value":"9000399355458"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/takanezawa"}],"foaf:name":[{"@value":"Yasukazu Takanezawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233482890496","@type":"Researcher","foaf:name":[{"@value":"Ryosuke Nakamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233482890629","@type":"Researcher","foaf:name":[{"@value":"Ryohei Harada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233482890633","@type":"Researcher","foaf:name":[{"@value":"Yuka Sone"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233482890752","@type":"Researcher","foaf:name":[{"@value":"Shimpei Uraguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233482890758","@type":"Researcher","foaf:name":[{"@value":"Masako Kiyono"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20452322"}],"prism:publicationName":[{"@value":"Scientific Reports"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2017-12-01","prism:volume":"7","prism:number":"1","prism:startingPage":"16735"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/s41598-017-17112-8.pdf"},{"@id":"https://www.nature.com/articles/s41598-017-17112-8"}],"createdAt":"2017-11-27","modifiedAt":"2022-12-23","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Confocal","dc:title":"Microscopy, Confocal"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Survival","dc:title":"Cell Survival"},{"@id":"https://cir.nii.ac.jp/all?q=Science","dc:title":"Science"},{"@id":"https://cir.nii.ac.jp/all?q=Q","dc:title":"Q"},{"@id":"https://cir.nii.ac.jp/all?q=R","dc:title":"R"},{"@id":"https://cir.nii.ac.jp/all?q=Chloroquine","dc:title":"Chloroquine"},{"@id":"https://cir.nii.ac.jp/all?q=Fibroblasts","dc:title":"Fibroblasts"},{"@id":"https://cir.nii.ac.jp/all?q=Methylmercury%20Compounds","dc:title":"Methylmercury Compounds"},{"@id":"https://cir.nii.ac.jp/all?q=Ubiquitinated%20Proteins","dc:title":"Ubiquitinated Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Article","dc:title":"Article"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Sequestosome-1%20Protein","dc:title":"Sequestosome-1 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