{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004233962451968.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.1600519113"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.1600519113"}},{"identifier":{"@type":"PMID","@value":"27071117"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Conductance of P2X<sub>4</sub>purinergic receptor is determined by conformational equilibrium in the transmembrane region"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Significance</jats:title><jats:p>Partial agonists of ligand-gated ion channels reportedly offer clinical advantages over antagonists and full agonists in antidepressant and smoking-cessation treatment. In the cases of P2X purinergic receptors, the currents evoked by α,β-methylene ATP are lower than the currents evoked by ATP. Here, our NMR analyses revealed that the transmembrane region and the membrane side of the lower body exist in conformational equilibrium between the closed and open conformations, with slower exchange rates than the chemical shift difference (<100 s<jats:sup>-1</jats:sup>), and that the small population of the open conformation of zebrafish P2X<jats:sub>4</jats:sub>purinergic receptor causes the partial activation in the α,β-methylene ATP-bound state. These findings provide insights into the mechanism underlying the partial activation of P2X<jats:sub>4</jats:sub>receptors and other ligand-gated ion channels.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004233962451970","@type":"Researcher","foaf:name":[{"@value":"Yuichi Minato"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962452099","@type":"Researcher","foaf:name":[{"@value":"Shiho Suzuki"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962452096","@type":"Researcher","foaf:name":[{"@value":"Tomoaki Hara"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276178792192","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80737940"},{"@type":"NRID","@value":"1000080737940"},{"@type":"NRID","@value":"9000326264470"},{"@type":"NRID","@value":"9000242667006"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/yutakakofuku"}],"foaf:name":[{"@value":"Yutaka Kofuku"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962451971","@type":"Researcher","foaf:name":[{"@value":"Go Kasuya"}],"jpcoar:affiliationName":[{"@value":"Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, 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Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962451973","@type":"Researcher","foaf:name":[{"@value":"Shunsuke Igarashi"}],"jpcoar:affiliationName":[{"@value":"Institute for Innovation, Ajinomoto, Kawasaki 210-8681, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962452097","@type":"Researcher","foaf:name":[{"@value":"Ei-ichiro Suzuki"}],"jpcoar:affiliationName":[{"@value":"Institute for Innovation, Ajinomoto, Kawasaki 210-8681, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962451972","@type":"Researcher","foaf:name":[{"@value":"Osamu Nureki"}],"jpcoar:affiliationName":[{"@value":"Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004233962451969","@type":"Researcher","foaf:name":[{"@value":"Motoyuki Hattori"}],"jpcoar:affiliationName":[{"@value":"State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Yangpu District, Shanghai 200438, China;"},{"@value":"Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0075, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326218387456","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20451859"},{"@type":"NRID","@value":"1000020451859"},{"@type":"NRID","@value":"9000002741015"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/TakumiUeda"}],"foaf:name":[{"@value":"Takumi Ueda"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;"},{"@value":"Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0075, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801200192256","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70196476"},{"@type":"NRID","@value":"1000070196476"},{"@type":"NRID","@value":"9000002735490"},{"@type":"NRID","@value":"9000241964850"},{"@type":"NRID","@value":"9000391644675"},{"@type":"NRID","@value":"9000253201186"},{"@type":"NRID","@value":"9000000414115"},{"@type":"NRID","@value":"9000020874483"},{"@type":"NRID","@value":"9000253181398"},{"@type":"NRID","@value":"9000253176897"},{"@type":"NRID","@value":"9000391626712"},{"@type":"NRID","@value":"9000379561242"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0206844"}],"foaf:name":[{"@value":"Ichio Shimada"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, 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