{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004235458769664.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/1574-6968.12023"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/femsle/article-pdf/337/2/155/19124806/337-2-155.pdf"}},{"identifier":{"@type":"PMID","@value":"23050832"}},{"identifier":{"@type":"HANDLE","@value":"11588/516620"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Copper induction of enhanced green fluorescent protein expression in<i>Pleurotus ostreatus</i>driven by laccase<i>poxa1b</i>promoter"}],"description":[{"notation":[{"@value":"In silico analyses of several laccase promoter sequences have shown the presence of many different responsive elements differentially distributed along the promoter sequences. Analysis of Pleurotus ostreatus laccase promoter poxa1b extending around 1400-bp upstream of the start codon showed the presence of several putative response elements, such as 10 metal-responsive elements. Development of a system for in vivo analysis of P. ostreatus laccase promoter poxa1b by enhanced green fluorescent protein expression was carried out, based on a polyethylene glycol-mediated procedure for fungal transformation. Quantitative measurement of fluorescence expressed in P. ostreatus transformants grown in the presence and in the absence of copper sulfate was performed, demonstrating an increase in expression level induced by the metal."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004235458769668","@type":"Researcher","foaf:name":[{"@value":"Antonella Amore"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical Sciences; University of Naples ‘Federico II’; Complesso Universitario Monte S. 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Italy"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751142631552","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70252517"},{"@type":"NRID","@value":"1000070252517"},{"@type":"ORCID","@value":"0000-0002-7218-7367"},{"@type":"CINII_AUTHOR_ID","@value":"DA16944866"},{"@type":"URI","@value":"https://ci.nii.ac.jp/author/DA16944866#entity"},{"@type":"URI","@value":"https://viaf.org/viaf/NII%7CDA16944866"},{"@type":"NRID","@value":"9000002216015"},{"@type":"NRID","@value":"9000287286525"},{"@type":"NRID","@value":"9000406058896"},{"@type":"NRID","@value":"9000405674810"},{"@type":"NRID","@value":"9000242070138"},{"@type":"NRID","@value":"9000392004323"},{"@type":"NRID","@value":"9000242547400"},{"@type":"NRID","@value":"9000392004316"},{"@type":"NRID","@value":"9000004162174"},{"@type":"NRID","@value":"9000392003497"},{"@type":"NRID","@value":"9000316223288"},{"@type":"NRID","@value":"9000006339840"},{"@type":"NRID","@value":"9000308368906"},{"@type":"NRID","@value":"9000261044695"},{"@type":"NRID","@value":"9000242447617"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0042714"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/yoichihonda"}],"foaf:name":[{"@value":"Yoichi Honda"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Agriculture; Kyoto University; Kyoto; Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235458769795","@type":"Researcher","foaf:name":[{"@value":"Vincenza Faraco"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03781097"}],"prism:publicationName":[{"@value":"FEMS Microbiology Letters"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2012-10-29","prism:volume":"337","prism:number":"2","prism:startingPage":"155","prism:endingPage":"163"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://academic.oup.com/femsle/article-pdf/337/2/155/19124806/337-2-155.pdf"}],"createdAt":"2012-10-11","modifiedAt":"2022-01-30","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Copper%20Sulfate","dc:title":"Copper Sulfate"},{"@id":"https://cir.nii.ac.jp/all?q=Green%20Fluorescent%20Proteins","dc:title":"Green Fluorescent Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Laccase","dc:title":"Laccase"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression","dc:title":"Gene Expression"},{"@id":"https://cir.nii.ac.jp/all?q=Biosensing%20Techniques","dc:title":"Biosensing Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Pleurotus","dc:title":"Pleurotus"},{"@id":"https://cir.nii.ac.jp/all?q=Promoter%20Regions,%20Genetic","dc:title":"Promoter Regions, Genetic"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782083226112","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21580402"},{"@type":"JGN","@value":"JP21580402"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580402/"}],"notation":[{"@language":"ja","@value":"リグニン分解酵素は、なぜ異種発現できないのか?"},{"@language":"en","@value":"Characterization of unique properties in expression of ligninolytic enzymes by white-rot fungi"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360292620802443904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Gene, Protein and Glycan Structures of Laccase from <i>Pleurotus ostreatus</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656135012992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707122745600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Introducing multiple-gene mutations in <i>Pleurotus ostreatus</i> using a polycistronic tRNA and CRISPR guide RNA strategy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993417116544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Metal-regulated 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