{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004235473486592.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/anu.12074"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fanu.12074"}},{"identifier":{"@type":"URI","@value":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/anu.12074/fullpdf"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Studies on transcription initiated by cauliflower mosaic virus 35S promoter from transgenic crops using fish cell lines (HINAE, YO-K, RTG-2) and rainbow trout<i>Oncorhynchus mykiss</i>"}],"description":[{"notation":[{"@value":"Cauliflower mosaic virus 35S promoter (CaMV 35S) used in transgenic crops has been suspected to be active in animal cells. This study examined transfection of constructs for expression of green fluorescent protein (GFP), including CaMV 35S. Cytomegalovirus (CMV) promoter and promoterless constructs were used as positive and negative control, respectively. These constructs were transfected to kidney cells (YO-K) and embryonic cells (HINAE) derived from Japanese flounder and gonad cells (RTG-2) derived from rainbow trout. In addition, plasmid solution was injected intramuscularly into rainbow trout or offered by feed for consecutive 14 days to examine the distribution of GFP in tissues. GFP signal was detected in all treatments that used lipofection during transfection of plasmids into fish cell lines. CMV promoter induced a higher GFP expression in all cell types compared with CaMV 35S. The in vivo study showed that GFP controlled by CaMV 35S was only detected at the injection site, despite the huge number of copies injected. The CaMV 35S was only able to drive one-third of GFP mRNA copies driven by CMV promoter. Administration of plasmid by feed was inefficient for transfection due to rapid degradation of plasmid DNA. DNA fragments (71 and 203 bp) derived from feed were detected in liver, kidney and spleen, while longer sequence (1224 bp) containing genetic information for GFP transcription was only detected in the gut until 24 h. CaMV 35S DNA fragment (203 bp) was detected until 48 h in kidney and spleen. The study showed that CaMV 35S was able to drive limited expression of GFP in fish cell lines and after intramuscular injection. Moreover, it was proposed that dietary DNA fragments, including the CaMV 35S sequence, were absorbed by the rainbow trout intestine but were cleaved into smaller fragments and consequently eliminated through metabolism or excretion."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004235473486348","@type":"Researcher","foaf:name":[{"@value":"R.E. Kitagima"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Marine Science and Technology; Tokyo University of Marine Science and Technology; Tokyo; Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235473486468","@type":"Researcher","foaf:name":[{"@value":"Y. Haga"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Marine Science and Technology; Tokyo University of Marine Science and Technology; Tokyo; Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235473486473","@type":"Researcher","foaf:name":[{"@value":"I. Hirono"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Marine Science and Technology; Tokyo University of Marine Science and Technology; Tokyo; Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235473486597","@type":"Researcher","foaf:name":[{"@value":"M. Endo"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Marine Science and Technology; Tokyo University of Marine Science and Technology; Tokyo; Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235473486346","@type":"Researcher","foaf:name":[{"@value":"S. Satoh"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Marine Science and Technology; Tokyo University of Marine Science and Technology; Tokyo; Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13535773"}],"prism:publicationName":[{"@value":"Aquaculture Nutrition"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2013-08","prism:volume":"19","prism:startingPage":"122","prism:endingPage":"134"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://doi.wiley.com/10.1002/tdm_license_1.1"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fanu.12074"},{"@id":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/anu.12074/fullpdf"}],"createdAt":"2013-09-02","modifiedAt":"2024-05-17","project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782111893248","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22380116"},{"@type":"JGN","@value":"JP22380116"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380116/"}],"notation":[{"@language":"ja","@value":"新しい養魚用無魚粉飼料の開発に関する基礎的研究"},{"@language":"en","@value":"Studies on development for new non-fish meal feed for aquaculture"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004231543029760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Suitability of genetically modified soybean meal in a dietary ingredient for common carp Cyprinus carpio"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143945001984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The effects of promoter on transient expression in conifer cell lines"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143965160576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144073918848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Oral delivery of DNA construct using chitosan nanoparticles to protect the shrimp from white spot syndrome virus (WSSV)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145965801984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"On the fate of plant or other foreign genes upon the uptake in food or after intramuscular injection in mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619330427264","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Specific uptake of plasmid DNA without reporter gene expression in Atlantic salmon (Salmo salar L.) kidney after intramuscular administration"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619373987200","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant 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