{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004235586500992.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1365-2982.2011.01767.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2982.2011.01767.x"}},{"identifier":{"@type":"URI","@value":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2982.2011.01767.x/fullpdf"}},{"identifier":{"@type":"PMID","@value":"21883699"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Acid inhibits TRPV4-mediated Ca2+ influx in mouse esophageal epithelial cells"}],"dcterms:alternative":[{"@value":"Acid-sensitive TRPV4 in the esophageal epithelium"}],"description":[{"notation":[{"@value":"The transient receptor potential vanilloid 4 (TRPV4), a thermo-sensitive stretch-activated cation channel, is expressed in the skin stratified squamous epithelium, contributing to the acquisition of barrier function. Similarly, functional TRPV4 may be located in the stratified squamous epithelial lining of the esophagus, being involved in the pathogenesis of gastroesophageal reflux disease (GERD). Here we investigated the expression of TRPV4 in the mouse esophageal epithelium.TRPV4 expression at the mRNA and protein levels was examined by reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, and immunohistochemistry. A calcium imaging technique and ATP assay were used to evaluate the functionality of TRPV4 in freshly isolated esophageal epithelial cells.Transcripts and proteins encoding TRPV4 were colocalized in the basal and intermediate layers of the esophageal epithelium. Both 4α-phorbol 12,13- didecanoate (4α-PDD), a selective agonist for TRPV4, and hypo-osmolar solution (160 mOsm) elevated the intracellular calcium concentration ([Ca(2+) ](i) ) in a subset of the isolated cells (70%). These [Ca(2+) ](i) increases were potently inhibited by ruthenium red (RuR), a TRPV4 channel antagonist, and were suppressed by extracellular protons (pH 5.0). Finally, application of 4α-PDD evoked ATP release in primary esophageal epithelial cells.Acid-sensitive TRPV4 channels were mainly expressed in the esophageal epithelial cells of the basal and intermediate layers. Direct exposure of TRPV4-expressing cells to gastric acid, as would occur in cases of GERD, could influence their cellular functions, possibly aggravating the disease state."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004235586500868","@type":"Researcher","foaf:name":[{"@value":"M. Shikano"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500618","@type":"Researcher","foaf:name":[{"@value":"T. Ueda"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500492","@type":"Researcher","foaf:name":[{"@value":"T. Kamiya"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500736","@type":"Researcher","foaf:name":[{"@value":"Y. Ishida"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500619","@type":"Researcher","foaf:name":[{"@value":"T. Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500480","@type":"Researcher","foaf:name":[{"@value":"T. Mizushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500616","@type":"Researcher","foaf:name":[{"@value":"T. Shimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500871","@type":"Researcher","foaf:name":[{"@value":"T. Mizoshita"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500482","@type":"Researcher","foaf:name":[{"@value":"S. Tanida"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500866","@type":"Researcher","foaf:name":[{"@value":"H. Kataoka"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500488","@type":"Researcher","foaf:name":[{"@value":"S. Shimada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500870","@type":"Researcher","foaf:name":[{"@value":"S. Ugawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235586500865","@type":"Researcher","foaf:name":[{"@value":"T. Joh"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13501925"}],"prism:publicationName":[{"@value":"Neurogastroenterology & Motility"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2011-08-23","prism:volume":"23","prism:number":"11","prism:startingPage":"1020","prism:endingPage":"e497"},"reviewed":"false","dc:rights":["http://doi.wiley.com/10.1002/tdm_license_1.1"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2982.2011.01767.x"},{"@id":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2982.2011.01767.x/fullpdf"}],"createdAt":"2011-08-31","modifiedAt":"2021-07-06","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=TRPV%20Cation%20Channels","dc:title":"TRPV Cation Channels"},{"@id":"https://cir.nii.ac.jp/all?q=Epithelial%20Cells","dc:title":"Epithelial Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20C57BL","dc:title":"Mice, Inbred C57BL"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Esophagus","dc:title":"Esophagus"},{"@id":"https://cir.nii.ac.jp/all?q=HEK293%20Cells","dc:title":"HEK293 Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Calcium","dc:title":"Calcium"},{"@id":"https://cir.nii.ac.jp/all?q=Acids","dc:title":"Acids"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257060401280","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21590238"},{"@type":"JGN","@value":"JP21590238"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590238/"}],"notation":[{"@language":"ja","@value":"呼吸上皮に存在する新規CO_2受容体の単離と機能解析"},{"@language":"en","@value":"Isolation and functional expression of novel putative carbon dioxide(CO_2) receptors in respiratory epithelium."}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257061377536","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21590791"},{"@type":"JGN","@value":"JP21590791"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590791/"}],"notation":[{"@language":"ja","@value":"食道知覚におけるTRPイオンチャンネルの機能解析"},{"@language":"en","@value":"The role of functional TRP ion channels on esophageal perception"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004237569799296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression and Regulation of Cav3.2 T-Type Calcium Channels during Inflammatory Hyperalgesia in Mouse Dorsal Root Ganglion Neurons"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144516955520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Physiology and Pathophysiology of Purinergic 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