{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004235967586816.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aem.01574-15"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AEM.01574-15"}},{"identifier":{"@type":"PMID","@value":"26319877"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Unique Plasmids Generated via pUC Replicon Mutagenesis in an Error-Prone Thermophile Derived from Geobacillus kaustophilus HTA426"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The plasmid pGKE75-\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            , which comprises pUC18 and the\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            gene encoding thermostable chloramphenicol acetyltransferase with an A138T amino acid replacement (CAT\n            <jats:sub>A138T</jats:sub>\n            ), serves as an\n            <jats:named-content content-type=\"genus-species\">Escherichia coli-Geobacillus kaustophilus</jats:named-content>\n            shuttle plasmid that confers moderate chloramphenicol resistance on\n            <jats:named-content content-type=\"genus-species\">G. kaustophilus</jats:named-content>\n            HTA426. The present study examined the thermoadaptation-directed mutagenesis of pGKE75-\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            in an error-prone thermophile, generating the mutant plasmid pGKE75\n            <jats:sup>αβ</jats:sup>\n            -\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            responsible for substantial chloramphenicol resistance at 65°C. pGKE75\n            <jats:sup>αβ</jats:sup>\n            -\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            contained no mutation in the\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            gene but had two mutations in the pUC replicon, even though the replicon has no apparent role in\n            <jats:named-content content-type=\"genus-species\">G. kaustophilus</jats:named-content>\n            . Biochemical characterization suggested that the efficient chloramphenicol resistance conferred by pGKE75\n            <jats:sup>αβ</jats:sup>\n            -\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            is attributable to increases in intracellular CAT\n            <jats:sub>A138T</jats:sub>\n            and acetyl-coenzyme A following a decrease in incomplete forms of pGKE75\n            <jats:sup>αβ</jats:sup>\n            -\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            . The decrease in incomplete plasmids may be due to optimization of plasmid replication by RNA species transcribed from the mutant pUC replicon, which were actually produced in\n            <jats:named-content content-type=\"genus-species\">G. kaustophilus</jats:named-content>\n            . It is noteworthy that\n            <jats:named-content content-type=\"genus-species\">G. kaustophilus</jats:named-content>\n            was transformed with pGKE75\n            <jats:sup>αβ</jats:sup>\n            -\n            <jats:italic>\n              cat\n              <jats:sub>A138T</jats:sub>\n            </jats:italic>\n            using chloramphenicol selection at 60°C. In addition, a pUC18 derivative with the two mutations propagated in\n            <jats:named-content content-type=\"genus-species\">E. coli</jats:named-content>\n            at a high copy number independently of the culture temperature and high plasmid stability. Since these properties have not been observed in known plasmids, the outcomes extend the genetic toolboxes for\n            <jats:named-content content-type=\"genus-species\">G. kaustophilus</jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">E. coli</jats:named-content>\n            .\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004235967586560","@type":"Researcher","foaf:name":[{"@value":"Jyumpei Kobayashi"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan"},{"@value":"Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235967586817","@type":"Researcher","foaf:name":[{"@value":"Misaki Tanabiki"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235967586688","@type":"Researcher","foaf:name":[{"@value":"Shohei Doi"}],"jpcoar:affiliationName":[{"@value":"Department of Biotechnology, Faculty of Engineering, Tottori University, Tottori, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235967586690","@type":"Researcher","foaf:name":[{"@value":"Akihiko Kondo"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Hyogo, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004235967586691","@type":"Researcher","foaf:name":[{"@value":"Takashi Ohshiro"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276167429888","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80462696"},{"@type":"NRID","@value":"1000080462696"},{"@type":"NRID","@value":"9000367821437"},{"@type":"NRID","@value":"9000001985415"},{"@type":"NRID","@value":"9000263058035"},{"@type":"NRID","@value":"9000399003462"},{"@type":"NRID","@value":"9000297473081"},{"@type":"NRID","@value":"9000001091495"},{"@type":"NRID","@value":"9000261040140"},{"@type":"NRID","@value":"9000242070111"},{"@type":"NRID","@value":"9000241204398"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0075769"}],"foaf:name":[{"@value":"Hirokazu Suzuki"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan"},{"@value":"Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1380004235967586689","@type":"Researcher","foaf:name":[{"@value":"H. Nojiri"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00992240"},{"@type":"EISSN","@value":"10985336"}],"prism:publicationName":[{"@value":"Applied and Environmental Microbiology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2015-11","prism:volume":"81","prism:number":"21","prism:startingPage":"7625","prism:endingPage":"7632"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AEM.01574-15"}],"createdAt":"2015-08-29","modifiedAt":"2022-02-23","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Chloramphenicol%20O-Acetyltransferase","dc:title":"Chloramphenicol O-Acetyltransferase"},{"@id":"https://cir.nii.ac.jp/all?q=Hot%20Temperature","dc:title":"Hot Temperature"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Vectors","dc:title":"Genetic 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