{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004236322428672.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajpheart.00241.2015"}},{"identifier":{"@type":"URI","@value":"https://www.physiology.org/doi/pdf/10.1152/ajpheart.00241.2015"}},{"identifier":{"@type":"PMID","@value":"26297225"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Vascular endothelial cell membranes differentiate between stretch and shear stress through transitions in their lipid phases"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p> Vascular endothelial cells (ECs) respond to the hemodynamic forces stretch and shear stress by altering their morphology, functions, and gene expression. However, how they sense and differentiate between these two forces has remained unknown. Here we report that the plasma membrane itself differentiates between stretch and shear stress by undergoing transitions in its lipid phases. Uniaxial stretching and hypotonic swelling increased the lipid order of human pulmonary artery EC plasma membranes, thereby causing a transition from the liquid-disordered phase to the liquid-ordered phase in some areas, along with a decrease in membrane fluidity. In contrast, shear stress decreased the membrane lipid order and increased membrane fluidity. A similar increase in lipid order occurred when the artificial lipid bilayer membranes of giant unilamellar vesicles were stretched by hypotonic swelling, indicating that this is a physical phenomenon. The cholesterol content of EC plasma membranes significantly increased in response to stretch but clearly decreased in response to shear stress. Blocking these changes in the membrane lipid order by depleting membrane cholesterol with methyl-β-cyclodextrin or by adding cholesterol resulted in a marked inhibition of the EC response specific to stretch and shear stress, i.e., phosphorylation of PDGF receptors and phosphorylation of VEGF receptors, respectively. These findings indicate that EC plasma membranes differently respond to stretch and shear stress by changing their lipid order, fluidity, and cholesterol content in opposite directions and that these changes in membrane physical properties are involved in the mechanotransduction that activates membrane receptors specific to each force. </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276180516352","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00323618"},{"@type":"NRID","@value":"1000000323618"},{"@type":"NRID","@value":"9000410167220"},{"@type":"NRID","@value":"9000018827508"},{"@type":"NRID","@value":"9000017041515"},{"@type":"NRID","@value":"9000405886941"},{"@type":"NRID","@value":"9000409859538"},{"@type":"NRID","@value":"9000006732142"},{"@type":"NRID","@value":"9000392138078"},{"@type":"NRID","@value":"9000006869312"},{"@type":"NRID","@value":"9000001443313"},{"@type":"NRID","@value":"9000257983392"},{"@type":"NRID","@value":"9000347074792"},{"@type":"NRID","@value":"9000327100206"},{"@type":"NRID","@value":"9000257807205"},{"@type":"NRID","@value":"9000018494708"},{"@type":"NRID","@value":"9000392138152"},{"@type":"NRID","@value":"9000265253736"},{"@type":"NRID","@value":"9000017430306"},{"@type":"NRID","@value":"9000398638912"},{"@type":"NRID","@value":"9000017554141"},{"@type":"NRID","@value":"9000021863124"},{"@type":"NRID","@value":"9000310345676"},{"@type":"NRID","@value":"9000415429594"},{"@type":"NRID","@value":"9000398150010"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/0358413564"}],"foaf:name":[{"@value":"Kimiko Yamamoto"}],"jpcoar:affiliationName":[{"@value":"Laboratory of System Physiology, Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo, Japan; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236322428928","@type":"Researcher","foaf:name":[{"@value":"Joji Ando"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Biomedical Engineering, School of Medicine, Dokkyo Medical University, Tochigi, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03636135"},{"@type":"EISSN","@value":"15221539"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Heart and Circulatory Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"2015-10","prism:volume":"309","prism:number":"7","prism:startingPage":"H1178","prism:endingPage":"H1185"},"reviewed":"false","url":[{"@id":"https://www.physiology.org/doi/pdf/10.1152/ajpheart.00241.2015"}],"createdAt":"2015-08-21","modifiedAt":"2019-09-08","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Membrane%20Fluidity","dc:title":"Membrane Fluidity"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Membrane","dc:title":"Cell Membrane"},{"@id":"https://cir.nii.ac.jp/all?q=beta-Cyclodextrins","dc:title":"beta-Cyclodextrins"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelial%20Cells","dc:title":"Endothelial Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Pulmonary%20Artery","dc:title":"Pulmonary Artery"},{"@id":"https://cir.nii.ac.jp/all?q=Mechanotransduction,%20Cellular","dc:title":"Mechanotransduction, Cellular"},{"@id":"https://cir.nii.ac.jp/all?q=Membrane%20Lipids","dc:title":"Membrane Lipids"},{"@id":"https://cir.nii.ac.jp/all?q=Cholesterol","dc:title":"Cholesterol"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Vascular%20Endothelial%20Growth%20Factor","dc:title":"Receptors, Vascular Endothelial Growth Factor"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Platelet-Derived%20Growth%20Factor","dc:title":"Receptors, Platelet-Derived Growth Factor"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelium,%20Vascular","dc:title":"Endothelium, Vascular"},{"@id":"https://cir.nii.ac.jp/all?q=Stress,%20Mechanical","dc:title":"Stress, Mechanical"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphorylation","dc:title":"Phosphorylation"},{"@id":"https://cir.nii.ac.jp/all?q=Shear%20Strength","dc:title":"Shear Strength"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257217585536","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"25282128"},{"@type":"JGN","@value":"JP25282128"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25282128/"}],"notation":[{"@language":"ja","@value":"流れずり応力に対する内皮細胞形質膜の力学応答"},{"@language":"en","@value":"Mechanoresponses of endothelial cell membranes to fluid shear stress"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257257866368","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"26242043"},{"@type":"JGN","@value":"JP26242043"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26242043/"}],"notation":[{"@language":"ja","@value":"血管細胞における血流のメカノトランスダクション機構"},{"@language":"en","@value":"Blood flow mechanotransduction in vascular endothelial cells"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050564287491768704","@type":"Article","resourceType":"学術雑誌論文(journal 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