{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004236326467456.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajplung.00428.2015"}},{"identifier":{"@type":"URI","@value":"https://www.physiology.org/doi/pdf/10.1152/ajplung.00428.2015"}},{"identifier":{"@type":"PMID","@value":"27343195"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"MiR-196a regulates heme oxygenase-1 by silencing Bach1 in the neonatal mouse lung"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p> In the lung, heme oxygenase-1 (HO-1) is developmentally regulated, with its highest expression in the first days of life. In addition, neonatal mice have limited HO-1 induction in hyperoxia compared with adults. However, few reports have addressed the functional effect of microRNAs (miRNAs) in the regulation of HO-1 in vivo. The aims of the present study were to characterize changes in lung miRNA expression during postnatal development and in response to hyperoxic exposure, and to identify miRNAs that target lung HO-1 gene expression. Neonatal (<12 h old) and adult (2 mo old) mice were exposed to room air or hyperoxia (95% oxygen) for 72 h. TaqMan low-density array rodent miRNA assays were used to calculate miRNA expression changes between control and hyperoxia groups in neonatal and adult lungs. In neonates, we identified miR-196a, which binds to the 3′-untranslated region of the transcriptional repressor BTB and CNC homology 1 (Bach1) and regulates its expression, and subsequently leads to higher levels of lung HO-1 mRNA compared with levels in adults. Despite the increase at baseline, miR-196a was degraded in hyperoxia resulting in limited HO-1 induction in neonatal mice lungs. Furthermore, the developmental differences in lung HO-1 gene expression can be explained in part by the variation in miRNA-196a and its effect on Bach1. This report is the first to show developmental differences in lung miR-196a and its effect on Bach1 and HO-1 expression at baseline and in hyperoxia. </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801185164544","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30443857"},{"@type":"NRID","@value":"1000030443857"},{"@type":"NRID","@value":"9000001239920"},{"@type":"NRID","@value":"9000345192296"},{"@type":"NRID","@value":"9000338422285"},{"@type":"NRID","@value":"9000401563498"}],"foaf:name":[{"@value":"Hayato Go"}],"jpcoar:affiliationName":[{"@value":"Department of Neonatology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania;"},{"@value":"Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467587","@type":"Researcher","foaf:name":[{"@value":"Ping La"}],"jpcoar:affiliationName":[{"@value":"Department of Neonatology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467457","@type":"Researcher","foaf:name":[{"@value":"Fumihiko Namba"}],"jpcoar:affiliationName":[{"@value":"Department of Neonatology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania;"},{"@value":"Department of Pediatrics, Saitama Medical Center, Saitama, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467456","@type":"Researcher","foaf:name":[{"@value":"Masato Ito"}],"jpcoar:affiliationName":[{"@value":"Department of Pediatrics, Saitama Medical Center, Saitama, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467584","@type":"Researcher","foaf:name":[{"@value":"Guang Yang"}],"jpcoar:affiliationName":[{"@value":"Department of Neonatology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467588","@type":"Researcher","foaf:name":[{"@value":"Andrey Brydun"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467585","@type":"Researcher","foaf:name":[{"@value":"Kazuhiko Igarashi"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236326467586","@type":"Researcher","foaf:name":[{"@value":"Phyllis A. Dennery"}],"jpcoar:affiliationName":[{"@value":"Department of Neonatology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania;"},{"@value":"Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania and Alpert Medical School at Brown University, Providence, Rhode Island; and"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10400605"},{"@type":"EISSN","@value":"15221504"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Lung Cellular and Molecular Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"2016-08-01","prism:volume":"311","prism:number":"2","prism:startingPage":"L400","prism:endingPage":"L411"},"reviewed":"false","url":[{"@id":"https://www.physiology.org/doi/pdf/10.1152/ajplung.00428.2015"}],"createdAt":"2016-06-26","modifiedAt":"2019-09-09","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Knockout","dc:title":"Mice, 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