{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004236347432192.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1155/2014/437871"}},{"identifier":{"@type":"URI","@value":"http://downloads.hindawi.com/journals/bmri/2014/437871.pdf"}},{"identifier":{"@type":"URI","@value":"http://downloads.hindawi.com/journals/bmri/2014/437871.xml"}},{"identifier":{"@type":"PMID","@value":"24995295"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Development of Pathological Diagnostics of Human Kidney Cancer by Multiple Staining Using New Fluorescent Fluolid Dyes"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>New fluorescent Fluolid dyes have advantages over others such as stability against heat, dryness, and excess light. Here, we performed simultaneous immunostaining of renal tumors, clear cell renal cell carcinoma (RCC), papillary RCC, chromophobe RCC, acquired cystic disease-associated RCC (ACD-RCC), and renal angiomyolipoma (AML), with primary antibodies against Kank1, cytokeratin 7 (CK7), and CD10, which were detected with secondary antibodies labeled with Fluolid-Orange, Fluolid-Green, and Alexa Fluor 647, respectively. Kank1 was stained in normal renal tubules, papillary RCC, and ACD-RCC, and weakly or negatively in all other tumors. CK7 was positive in normal renal tubules, papillary RCC, and ACD-RCC. In contrast, CD10 was expressed in renal tubules and clear cell RCC, papillary RCC, AML, and AC-RCC, and weakly in chromophobe RCC. These results may contribute to differentiating renal tumors and subtypes of RCCs. We also examined the stability of fluorescence and found that fluorescent images of Fluolid dyes were identical between a tissue section and the same section after it was stored for almost three years at room temperature. This indicates that tissue sections can be stored at room temperature for a relatively long time after they are stained with multiple fluorescent markers, which could open a door for pathological diagnostics.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004236347432194","@type":"Researcher","foaf:name":[{"@value":"Dilibaier Wuxiuer"}],"jpcoar:affiliationName":[{"@value":"Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432193","@type":"Researcher","foaf:name":[{"@value":"Yun Zhu"}],"jpcoar:affiliationName":[{"@value":"Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432069","@type":"Researcher","foaf:name":[{"@value":"Takunori Ogaeri"}],"jpcoar:affiliationName":[{"@value":"Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432067","@type":"Researcher","foaf:name":[{"@value":"Keiji Mizuki"}],"jpcoar:affiliationName":[{"@value":"Department of Nanoscience, Faculty of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432192","@type":"Researcher","foaf:name":[{"@value":"Yuki Kashiwa"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Higashi-ku, Fukuoka 813-8503, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432065","@type":"Researcher","foaf:name":[{"@value":"Kentaro Nishi"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Higashi-ku, Fukuoka 813-8503, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432066","@type":"Researcher","foaf:name":[{"@value":"Shin-ichiro Isobe"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Higashi-ku, Fukuoka 813-8503, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004236347432064","@type":"Researcher","foaf:name":[{"@value":"Tei-ichiro Aoyagi"}],"jpcoar:affiliationName":[{"@value":"Ibaraki Medical Center, Tokyo Medical University, 3-20-1 Ami, Inashiki, Ibaraki 300-0395, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658343275776","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00240739"},{"@type":"NRID","@value":"1000000240739"},{"@type":"NRID","@value":"9000008477863"},{"@type":"NRID","@value":"9000002738889"},{"@type":"NRID","@value":"9000014891990"},{"@type":"NRID","@value":"9000000172126"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/kiyamar"}],"foaf:name":[{"@value":"Ryoiti Kiyama"}],"jpcoar:affiliationName":[{"@value":"Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"23146133"},{"@type":"EISSN","@value":"23146141"}],"prism:publicationName":[{"@value":"BioMed Research International"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2014","prism:volume":"2014","prism:startingPage":"1","prism:endingPage":"6"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://creativecommons.org/licenses/by/3.0/"],"url":[{"@id":"http://downloads.hindawi.com/journals/bmri/2014/437871.pdf"},{"@id":"http://downloads.hindawi.com/journals/bmri/2014/437871.xml"}],"createdAt":"2014-06-03","modifiedAt":"2022-04-05","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Tumor%20Suppressor%20Proteins","dc:title":"Tumor Suppressor Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Keratin-7","dc:title":"Keratin-7"},{"@id":"https://cir.nii.ac.jp/all?q=Antibodies","dc:title":"Antibodies"},{"@id":"https://cir.nii.ac.jp/all?q=Kidney%20Neoplasms","dc:title":"Kidney Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Cytoskeletal%20Proteins","dc:title":"Cytoskeletal Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Antigens,%20Neoplasm","dc:title":"Antigens, Neoplasm"},{"@id":"https://cir.nii.ac.jp/all?q=Biomarkers,%20Tumor","dc:title":"Biomarkers, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Neprilysin","dc:title":"Neprilysin"},{"@id":"https://cir.nii.ac.jp/all?q=Research%20Article","dc:title":"Research Article"},{"@id":"https://cir.nii.ac.jp/all?q=Adaptor%20Proteins,%20Signal%20Transducing","dc:title":"Adaptor Proteins, Signal Transducing"},{"@id":"https://cir.nii.ac.jp/all?q=Fluorescent%20Dyes","dc:title":"Fluorescent Dyes"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257172514560","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"24310050"},{"@type":"JGN","@value":"JP24310050"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24310050/"}],"notation":[{"@language":"ja","@value":"エストロゲン様化学物質影響評価のための細胞内新規シグナル伝達経路の解明"},{"@language":"en","@value":"The analysis of a novel intracellular signaling pathway to evaluate estrogenic chemicals"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215253384320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Kank proteins: structure, functions and diseases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002216806368256","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Integrated molecular analysis of clear-cell renal cell 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