{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004237486471680.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1371/journal.pone.0016617"}},{"identifier":{"@type":"URI","@value":"http://dx.plos.org/10.1371/journal.pone.0016617"}},{"identifier":{"@type":"PMID","@value":"21304604"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"MicroRNA-126 Inhibits SOX2 Expression and Contributes to Gastric Carcinogenesis"}],"description":[{"notation":[{"@value":"SRY (sex-determining region Y)-box 2 (SOX2) is a crucial transcription factor for the maintenance of embryonic stem cell pluripotency and the determination of cell fate. Previously, we demonstrated that SOX2 plays important roles in growth inhibition through cell cycle arrest and apoptosis, and that SOX2 expression is frequently down-regulated in gastric cancers. However, the mechanisms underlying loss of SOX2 expression and its target genes involved in gastric carcinogenesis remain largely unknown. Here, we assessed whether microRNAs (miRNAs) regulate SOX2 expression in gastric cancers. Furthermore, we attempted to find downstream target genes of SOX2 contributing to gastric carcinogenesis.We performed in silico analysis and focused on miRNA-126 (miR-126) as a potential SOX2 regulator. Gain- and loss-of function experiments and luciferase assays revealed that miR-126 inhibited SOX2 expression by targeting two binding sites in the 3'-untranslated region (3'-UTR) of SOX2 mRNA in multiple cell lines. In addition, miR-126 was highly expressed in some cultured and primary gastric cancer cells with low SOX2 protein levels. Furthermore, exogenous miR-126 over-expression as well as siRNA-mediated knockdown of SOX2 significantly enhanced the anchorage-dependent and -independent growth of gastric cancer cell lines. We next performed microarray analysis after SOX2 over-expression in a gastric cancer cell line, and found that expression of the placenta-specific 1 (PLAC1) gene was significantly down-regulated by SOX2 over-expression. siRNA- and miR-126-mediated SOX2 knockdown experiments revealed that miR-126 positively regulated PLAC1 expression through suppression of SOX2 expression in gastric cancer cells.Taken together, our results indicate that miR-126 is a novel miRNA that targets SOX2, and PLAC1 may be a novel downstream target gene of SOX2 in gastric cancer cells. These findings suggest that aberrant over-expression of miR-126 and consequent SOX2 down-regulation may contribute to gastric carcinogenesis."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004237486471430","@type":"Researcher","foaf:name":[{"@value":"Takeshi Otsubo"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237486471298","@type":"Researcher","foaf:name":[{"@value":"Yoshimitsu Akiyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237486471435","@type":"Researcher","foaf:name":[{"@value":"Yutaka Hashimoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237486471297","@type":"Researcher","foaf:name":[{"@value":"Shu Shimada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237486471431","@type":"Researcher","foaf:name":[{"@value":"Kentaro Goto"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658319613697","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80111558"},{"@type":"NRID","@value":"1000080111558"},{"@type":"NRID","@value":"9000006176952"},{"@type":"NRID","@value":"9000021351249"},{"@type":"NRID","@value":"9000107381627"},{"@type":"NRID","@value":"9000383083950"},{"@type":"NRID","@value":"9000253567838"},{"@type":"NRID","@value":"9000392740221"},{"@type":"NRID","@value":"9000007278526"},{"@type":"NRID","@value":"9000399218714"},{"@type":"NRID","@value":"9000392750785"},{"@type":"NRID","@value":"9000392746114"},{"@type":"NRID","@value":"9000007345886"},{"@type":"NRID","@value":"9000001454546"},{"@type":"NRID","@value":"9000253401047"},{"@type":"NRID","@value":"9000253400972"},{"@type":"NRID","@value":"9000392746238"},{"@type":"NRID","@value":"9000253400813"},{"@type":"NRID","@value":"9000392749392"},{"@type":"NRID","@value":"9000009280880"},{"@type":"NRID","@value":"9000018350166"},{"@type":"NRID","@value":"9000020095270"},{"@type":"NRID","@value":"9000256626816"},{"@type":"NRID","@value":"9000008348255"},{"@type":"NRID","@value":"9000392743528"},{"@type":"NRID","@value":"9000398985592"}],"foaf:name":[{"@value":"Yasuhito Yuasa"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1380004237486471810","@type":"Researcher","foaf:name":[{"@value":"Guillaume Dalmasso"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"19326203"}],"prism:publicationName":[{"@value":"PLoS ONE"}],"dc:publisher":[{"@value":"Public Library of Science (PLoS)"}],"prism:publicationDate":"2011-01-27","prism:volume":"6","prism:number":"1","prism:startingPage":"e16617"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"http://dx.plos.org/10.1371/journal.pone.0016617"}],"createdAt":"2011-01-27","modifiedAt":"2024-04-03","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Binding%20Sites","dc:title":"Binding Sites"},{"@id":"https://cir.nii.ac.jp/all?q=Science","dc:title":"Science"},{"@id":"https://cir.nii.ac.jp/all?q=SOXB1%20Transcription%20Factors","dc:title":"SOXB1 Transcription Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Q","dc:title":"Q"},{"@id":"https://cir.nii.ac.jp/all?q=R","dc:title":"R"},{"@id":"https://cir.nii.ac.jp/all?q=Pregnancy%20Proteins","dc:title":"Pregnancy Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Neoplastic","dc:title":"Gene Expression Regulation, Neoplastic"},{"@id":"https://cir.nii.ac.jp/all?q=MicroRNAs","dc:title":"MicroRNAs"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Transformation,%20Neoplastic","dc:title":"Cell Transformation, Neoplastic"},{"@id":"https://cir.nii.ac.jp/all?q=Stomach%20Neoplasms","dc:title":"Stomach Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Medicine","dc:title":"Medicine"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"},{"@id":"https://cir.nii.ac.jp/all?q=3'%20Untranslated%20Regions","dc:title":"3' Untranslated Regions"},{"@id":"https://cir.nii.ac.jp/all?q=Research%20Article","dc:title":"Research Article"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782037094272","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"20390169"},{"@type":"JGN","@value":"JP20390169"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390169/"}],"notation":[{"@language":"ja","@value":"エピジェネティック疫学の疾病予防への応用"},{"@language":"en","@value":"Epigenetic epidemiology of gastric cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782083971456","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21590396"},{"@type":"JGN","@value":"JP21590396"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590396/"}],"notation":[{"@language":"ja","@value":"胃がんの臨床病理学的諸性状に関連したマイクロRNAの探索とメチル化異常の解析"},{"@language":"en","@value":"Clinicopathological significance and epigenetic alterations of miRNAs in gastric cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782146871168","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23300342"},{"@type":"JGN","@value":"JP23300342"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300342/"}],"notation":[{"@language":"ja","@value":"胃がんのマウスモデルとヒト血球ＤＮＡメチル化の解析"},{"@language":"en","@value":"Gastric cancer: a mouse model and epigenetic statuses of blood in mice and humans"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782491891200","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"11J04240"},{"@type":"JGN","@value":"JP11J04240"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11J04240/"}],"notation":[{"@language":"ja","@value":"胃がんにおけるmiRNAクラスターの発現制御機構とそのがん化との関連の解析"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257458164096","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"09J03751"},{"@type":"JGN","@value":"JP09J03751"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09J03751/"}],"notation":[{"@language":"ja","@value":"胃の分化及び胃癌発症における転写因子SOX2の発現調節機構と機能の解析"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011144172232064","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Elevating the levels of Sox2 in embryonal carcinoma cells and embryonic stem cells inhibits the expression of Sox2:Oct-3/4 target 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