{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004237845779072.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1515/hsz-2015-0176"}},{"identifier":{"@type":"URI","@value":"http://www.degruyter.com/view/j/bchm.2015.396.issue-11/hsz-2015-0176/hsz-2015-0176.xml"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"A simple and highly sensitive method of measuring heme oxygenase activity"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n<jats:p>Heme oxygenase (HO) is a rate-limiting step of heme degradation, which catalyzes the conversion of heme into biliverdin, iron, and CO. HO has been characterized in microorganisms, insects, plants, and mammals. Previously used assays of HO activity were complicated and had low sensitivity. We found that the use of an eel bilirubin-bound fluorescent protein, UnaG, can achieve a highly sensitive and simple assay of HO activity. Using several enzyme sources including human culture cells, homogenates of plant tissues, and recombinant yeast HO, data were successfully obtained. The present method can facilitate the examination of HO in various organisms.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004237845779202","@type":"Researcher","foaf:name":[{"@value":"Pham Hieu Liem"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237845779208","@type":"Researcher","foaf:name":[{"@value":"Anfeng Mu"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237845779073","@type":"Researcher","foaf:name":[{"@value":"Shun-ichi Kikuta"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237845779206","@type":"Researcher","foaf:name":[{"@value":"Kazushi Ohta"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237845779205","@type":"Researcher","foaf:name":[{"@value":"Sakihito Kitajima"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004237845779072","@type":"Researcher","foaf:name":[{"@value":"Shigeru Taketani"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14374315"},{"@type":"PISSN","@value":"14316730"}],"prism:publicationName":[{"@value":"Biological Chemistry"}],"dc:publisher":[{"@value":"Walter de Gruyter GmbH"}],"prism:publicationDate":"2015-11-01","prism:volume":"396","prism:number":"11","prism:startingPage":"1265","prism:endingPage":"1268"},"reviewed":"false","url":[{"@id":"http://www.degruyter.com/view/j/bchm.2015.396.issue-11/hsz-2015-0176/hsz-2015-0176.xml"}],"createdAt":"2015-06-03","modifiedAt":"2021-02-27","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Clinical%20Biochemistry","dc:title":"Clinical Biochemistry"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Biology","dc:title":"Molecular Biology"},{"@id":"https://cir.nii.ac.jp/all?q=Biochemistry","dc:title":"Biochemistry"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282256815305088","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15K00566"},{"@type":"JGN","@value":"JP15K00566"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K00566/"}],"notation":[{"@language":"ja","@value":"炭素安定同位体と遺伝的解析による気孔開度評価に基づいた街路樹の環境ストレス診断"},{"@language":"en","@value":"Evaluation of tolerance to environmental stresses for roadside tree species based on stomatal function, using stable carbon isotope ratio and genetic analysis"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360565166613506432","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Feedback Response to Selective Depletion of Endogenous Carbon Monoxide in the Blood"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993350257024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Heme transport from rat liver mitochondria to the microsomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943984895616","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bilirubin Chemistry and Metabolism; 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