{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004239431985280.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3389/fcimb.2018.00413"}},{"identifier":{"@type":"URI","@value":"https://www.frontiersin.org/article/10.3389/fcimb.2018.00413/full"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes"}],"description":[{"notation":[{"@value":"Within the liver, Plasmodium sporozoites traverse cells searching for a “suitable” hepatocyte, invading these cells through a process that results in the formation of a parasitophorous vacuole (PV), within which the parasite undergoes intracellular replication as a liver stage. It was previously established that two members of the Plasmodium s48/45 protein family, P36 and P52, are essential for productive invasion of host hepatocytes by sporozoites as their simultaneous deletion results in growth-arrested parasites that lack a PV. Recent studies point toward a pathway of entry possibly involving the interaction of P36 with hepatocyte receptors EphA2, CD81, and SR-B1. However, the relationship between P36 and P52 during sporozoite invasion remains unknown. Here we show that parasites with a single P52 or P36 gene deletion each lack a PV after hepatocyte invasion, thereby pheno-copying the lack of a PV observed for the P52/P36 dual gene deletion parasite line. This indicates that both proteins are equally important in the establishment of a PV and act in the same pathway. We created a Plasmodium yoelii P36(mCherry) tagged parasite line that allowed us to visualize the subcellular localization of P36 and found that it partially co-localizes with P52 in the sporozoite secretory microneme organelles. Furthermore, through co-immunoprecipitation studies in vivo, we determined that P36 and P52 form a protein complex in sporozoites, indicating a concerted function for both proteins within the PV formation pathway. However, upon sporozoite stimulation, only P36 was released as a secreted protein while P52 was not. Our results support a model in which the putatively glycosylphosphatidylinositol (GPI)-anchored P52 may serve as a scaffold to facilitate the interaction of secreted P36 with the host cell during sporozoite invasion of hepatocytes."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004239431984904","@type":"Researcher","foaf:name":[{"@value":"Silvia A. Arredondo"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985285","@type":"Researcher","foaf:name":[{"@value":"Kristian E. Swearingen"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985290","@type":"Researcher","foaf:name":[{"@value":"Thomas Martinson"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985287","@type":"Researcher","foaf:name":[{"@value":"Ryan Steel"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985417","@type":"Researcher","foaf:name":[{"@value":"Dorender A. Dankwa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985412","@type":"Researcher","foaf:name":[{"@value":"Anke Harupa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431984777","@type":"Researcher","foaf:name":[{"@value":"Nelly Camargo"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985157","@type":"Researcher","foaf:name":[{"@value":"William Betz"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431984768","@type":"Researcher","foaf:name":[{"@value":"Vladimir Vigdorovich"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431984642","@type":"Researcher","foaf:name":[{"@value":"Brian G. Oliver"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985154","@type":"Researcher","foaf:name":[{"@value":"Niwat Kangwanrangsan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801186201856","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40402680"},{"@type":"NRID","@value":"1000040402680"},{"@type":"NRID","@value":"9000005461109"},{"@type":"NRID","@value":"9000253177157"},{"@type":"NRID","@value":"9000000414627"},{"@type":"NRID","@value":"9000408632329"},{"@type":"NRID","@value":"9000002040293"},{"@type":"NRID","@value":"9000409848625"},{"@type":"NRID","@value":"9000411820433"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/sporozoite"}],"foaf:name":[{"@value":"Tomoko Ishino"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431984900","@type":"Researcher","foaf:name":[{"@value":"Noah Sather"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985033","@type":"Researcher","foaf:name":[{"@value":"Sebastian Mikolajczak"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431984778","@type":"Researcher","foaf:name":[{"@value":"Ashley M. Vaughan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431984776","@type":"Researcher","foaf:name":[{"@value":"Motomi Torii"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985409","@type":"Researcher","foaf:name":[{"@value":"Robert L. Moritz"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239431985162","@type":"Researcher","foaf:name":[{"@value":"Stefan H. I. Kappe"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"22352988"}],"prism:publicationName":[{"@value":"Frontiers in Cellular and Infection Microbiology"}],"dc:publisher":[{"@value":"Frontiers Media SA"}],"prism:publicationDate":"2018-11-27","prism:volume":"8","prism:startingPage":"413"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.frontiersin.org/article/10.3389/fcimb.2018.00413/full"}],"createdAt":"2018-11-27","modifiedAt":"2022-09-06","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Plasmodium","dc:title":"Plasmodium"},{"@id":"https://cir.nii.ac.jp/all?q=protein%20complex","dc:title":"protein complex"},{"@id":"https://cir.nii.ac.jp/all?q=malaria","dc:title":"malaria"},{"@id":"https://cir.nii.ac.jp/all?q=invasion","dc:title":"invasion"},{"@id":"https://cir.nii.ac.jp/all?q=Microbiology","dc:title":"Microbiology"},{"@id":"https://cir.nii.ac.jp/all?q=QR1-502","dc:title":"QR1-502"},{"@id":"https://cir.nii.ac.jp/all?q=Cellular%20and%20Infection%20Microbiology","dc:title":"Cellular and Infection Microbiology"},{"@id":"https://cir.nii.ac.jp/all?q=sporozoite","dc:title":"sporozoite"},{"@id":"https://cir.nii.ac.jp/all?q=6-cys%20s48/45","dc:title":"6-cys s48/45"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282256877484416","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16H05182"},{"@type":"JGN","@value":"JP16H05182"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H05182/"}],"notation":[{"@language":"ja","@value":"スポロゾイトの肝細胞侵入におけるRON3の作用機序の解明とレセプターの同定"},{"@language":"en","@value":"Elucidation of the mechanisms of RON3 during sporozoites invasion of hepatocytes"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011144201180800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A comprehensive evaluation of rodent malaria parasite genomes and gene expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145883258368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comet: An open‐source\n                    <scp>MS</scp>\n                    /\n                    <scp>MS</scp>\n                    sequence database search tool"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146170976640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An Overexpression Screen of Toxoplasma gondii Rab-GTPases Reveals Distinct Transport Routes to the Micronemes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146331757056","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Proteomic Analysis Identifies Novel Proteins of the Maurer's Clefts, a Secretory Compartment Delivering Plasmodium falciparum Proteins to the Surface of Its Host Cell"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620427790976","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The s48/45 six-cysteine proteins: mediators of interaction throughout the Plasmodium life cycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620615993600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"First-in-human evaluation of genetically attenuated Plasmodium falciparum sporozoites administered by bite of Anopheles mosquitoes to adult volunteers"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621071435008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mobilization of intracellular calcium stimulates microneme discharge in <i>Toxoplasma gondii</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574093871546368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Plasmodium berghei Δp52&p36 Parasites Develop Independent of a Parasitophorous Vacuole Membrane in Huh-7 Liver Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094112826624","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cellular interactions of Plasmodium liver stage with its host mammalian cell"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094617870720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Microneme proteins: structural and functional requirements to promote adhesion and invasion by the apicomplexan parasite Toxoplasma gondii"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094683900544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The journey of the malaria sporozoite through its hosts: two parasite proteins leadthe way"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095068859904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Total and Putative Surface Proteomics of Malaria Parasite Salivary Gland Sporozoites"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096580225792","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855567860266240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"NIH Image to ImageJ: 25 years of image analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568765226112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Induction and localization of Plasmodium falciparum stress proteins related to the heat shock protein 70 family"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569380940416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Assessing the adequacy of attenuation of genetically modified malaria parasite vaccine candidates"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569533898880","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569536459776","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"TRAP Is Necessary for Gliding Motility and Infectivity of Plasmodium Sporozoites"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569812708480","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Disruption of\n            <i>Plasmodium</i>\n            Sporozoite Transmission by Depletion of Sporozoite Invasion-Associated Protein 1"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570289444096","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570863539968","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Malaria Sporozoites Traverse Host Cells within Transient Vacuoles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571150560000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gliding motility and cell invasion by Apicomplexa: insights from the Plasmodium sporozoite. Microreview"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571359756416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Statistical Model for Identifying Proteins by Tandem Mass Spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593795974656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857596908450048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The parasitophorous vacuole membrane surrounding <i>Plasmodium</i> and <i>Toxoplasma:</i> An unusual compartment in infected cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044206011264","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Identification of additional members define a Plasmodium falciparum gene superfamily which includes Pfs48/45 and Pfs230"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044958440576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Discovery of Gene Function by Expression Profiling of the Malaria Parasite Life Cycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045104540416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519106237056","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Key molecular events during host cell invasion by Apicomplexan pathogens"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519927426048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Migration of\n            <i>Plasmodium</i>\n            Sporozoites Through Cells Before Infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520898726144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The moving junction of apicomplexan parasites: a key structure for invasion"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994769353472","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Two proteins with 6‐cys motifs are required for malarial parasites to commit to infection of the hepatocyte"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994859339392","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Genetically attenuated P36p-deficient Plasmodium berghei sporozoites confer long-lasting and partial cross-species protection"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994932309376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biochemical and Functional Analysis of Two Plasmodium falciparum Blood-Stage 6-Cys Proteins: P12 and P41"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995214576256","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Exploring the transcriptome of the malaria sporozoite stage"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995259287168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database Search"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469845885824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"iProphet: Multi-level Integrative Analysis of Shotgun Proteomic Data Improves Peptide and Protein Identification Rates and Error Estimates"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469947330176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Plasmodium liver-stage parasitophorous vacuole: A front-line of communication between parasite and host"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470013723264","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Trans‐Proteomic Pipeline, a standardized data processing pipeline for large‐scale reproducible proteomics informatics"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470669474432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Structure of Plasmodium falciparum Blood-Stage 6-Cys Protein Pf41 Reveals an Unexpected Intra-Domain Insertion Required for Pf12 Coordination"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470939479168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Refinements to Label Free Proteome Quantitation: How to Deal with Peptides Shared by Multiple Proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471284310272","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Three Members of the 6-cys Protein Family of Plasmodium Play a Role in Gamete Fertility"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945755374464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Characterization of Plasmodium falciparum sporozoite surface protein 2."}]},{"@id":"https://cir.nii.ac.jp/crid/1362262946128904576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544418350733952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Statistical Analysis of Membrane Proteome Expression Changes in <i>Saccharomyces </i><i>c</i><i>erevisiae</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419260091008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Apical organelles of Apicomplexa: biology and isolation by subcellular fractionation"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419396721280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Invited review Apical organelles and host-cell invasion by Apicomplexa"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419738949760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Malaria parasites target the hepatocyte receptor EphA2 for successful host infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419805994112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Type II fatty acid synthesis is essential only for malaria parasite late liver stage development"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544421202143744","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Secretion of micronemal proteins is associated with toxoplasma invasion of host cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894432295552","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Conservation of a Gliding Motility and Cell Invasion Machinery in Apicomplexan Parasites"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896064103424","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Defective sorting of the thrombospondin-related anonymous protein (TRAP) inhibits Plasmodium infectivity"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369367418112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"PlasmoDB: a functional genomic database for malaria parasites"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369982321920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and 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A2"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846050361856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Abacus: A computational tool for extracting and pre‐processing spectral count data for label‐free quantitative proteomic analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846155175552","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Plasmodium P36 determines host cell receptor usage during sporozoite invasion"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318504818560","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparative genomics in Plasmodium: a tool for the identification of genes and functional analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318741277568","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Preerythrocytic, live-attenuated\n            <i>Plasmodium falciparum</i>\n            vaccine candidates by 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