{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004239463515776.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3389/fpls.2018.01177"}},{"identifier":{"@type":"URI","@value":"https://www.frontiersin.org/article/10.3389/fpls.2018.01177/full"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Overexpression of a Brix Domain-Containing Ribosome Biogenesis Factor ARPF2 and its Interactor ARRS1 Causes Morphological Changes and Lifespan Extension in Arabidopsis thaliana"}],"description":[{"notation":[{"@value":"The Brix domain is a conserved domain in several proteins involved in ribosome biogenesis in yeast and animals. In the Arabidopsis genome, six Brix domain-containing proteins are encoded; however, their molecular functions have not been fully characterized, as yet. Here we report the functional analysis of a Brix domain-containing protein, ARPF2, which is homologous to yeast Rpf2 that plays an essential role in ribosome biogenesis as a component of the 5S ribonucleoprotein particle. By phenotypic characterization of arpf2 mutants, histochemical GUS staining, and analysis using green fluorescence protein, we show that ARPF2 is an essential and ubiquitously expressed gene encoding a nucleolar protein. Co-immunoprecipitation and split-GFP-based bimolecular fluorescence complementation assays revealed that ARPF2 interacts with a protein named ARRS1, which is homologous to yeast Rrs1 that forms a complex with Rpf2 in yeast. Furthermore, the result of RNA immunoprecipitation assay indicated that ARPF2 interacts with 5S ribosomal RNA (rRNA) or the precursor of 5S rRNA, as well as with the internal transcribed spacer 2 in the precursors of 25S rRNA. Most intriguingly, we found that the overexpression of ARPF2 and ARRS1 leads to characteristic phenotypes, including short stem, abnormal leaf morphology, and long lifespan, in Arabidopsis. These results suggest that the function of Brix domain-containing ARPF2 protein in ribosome biogenesis is intimately associated with the growth and development in plants."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276167670784","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80711209"},{"@type":"NRID","@value":"1000080711209"},{"@type":"NRID","@value":"9000375908579"},{"@type":"NRID","@value":"9000318569552"},{"@type":"NRID","@value":"9000375908413"},{"@type":"NRID","@value":"9000391957442"},{"@type":"NRID","@value":"9000391954806"},{"@type":"NRID","@value":"9000375908350"},{"@type":"NRID","@value":"9000391945860"},{"@type":"NRID","@value":"9000019194328"},{"@type":"NRID","@value":"9000391963694"},{"@type":"NRID","@value":"9000391946441"},{"@type":"NRID","@value":"9000391954788"},{"@type":"NRID","@value":"9000391945853"},{"@type":"NRID","@value":"9000391938845"},{"@type":"NRID","@value":"9000391954917"},{"@type":"NRID","@value":"9000286624414"},{"@type":"NRID","@value":"9000411952369"},{"@type":"NRID","@value":"9000391947148"},{"@type":"NRID","@value":"9000391975711"},{"@type":"NRID","@value":"9000396173139"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/mae-shu"}],"foaf:name":[{"@value":"Shugo Maekawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1030299673836222464","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000401558126"},{"@type":"NRID","@value":"9000405615247"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/yoshiakiueda"}],"foaf:name":[{"@value":"Yoshiaki Ueda"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751137030528","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20222359"},{"@type":"NRID","@value":"1000020222359"},{"@type":"NRID","@value":"9000000197490"},{"@type":"NRID","@value":"9000258724069"},{"@type":"NRID","@value":"9000290374839"},{"@type":"NRID","@value":"9000001480500"},{"@type":"NRID","@value":"9000366125956"},{"@type":"NRID","@value":"9000001132833"},{"@type":"NRID","@value":"9000001227125"},{"@type":"NRID","@value":"9000412099676"},{"@type":"NRID","@value":"9000018798142"},{"@type":"NRID","@value":"9000398406043"},{"@type":"NRID","@value":"9000290374801"},{"@type":"NRID","@value":"9000411952370"},{"@type":"NRID","@value":"9000017730151"},{"@type":"NRID","@value":"9000297508975"},{"@type":"NRID","@value":"9000401558128"},{"@type":"NRID","@value":"9000409975664"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0122105"}],"foaf:name":[{"@value":"Shuichi Yanagisawa"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"1664462X"}],"prism:publicationName":[{"@value":"Frontiers in Plant Science"}],"dc:publisher":[{"@value":"Frontiers Media SA"}],"prism:publicationDate":"2018-08-27","prism:volume":"9"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.frontiersin.org/article/10.3389/fpls.2018.01177/full"}],"createdAt":"2018-08-27","modifiedAt":"2019-10-23","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=yeast%20Rpf2%20homolog","dc:title":"yeast Rpf2 homolog"},{"@id":"https://cir.nii.ac.jp/all?q=Arabidopsis%20thaliana","dc:title":"Arabidopsis thaliana"},{"@id":"https://cir.nii.ac.jp/all?q=Brix%20domain","dc:title":"Brix domain"},{"@id":"https://cir.nii.ac.jp/all?q=ribosome%20biogenesis","dc:title":"ribosome biogenesis"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20culture","dc:title":"Plant culture"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Science","dc:title":"Plant Science"},{"@id":"https://cir.nii.ac.jp/all?q=lifespan","dc:title":"lifespan"},{"@id":"https://cir.nii.ac.jp/all?q=SB1-1110","dc:title":"SB1-1110"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781994202624","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18H03940"},{"@type":"JGN","@value":"JP18H03940"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H03940/"}],"notation":[{"@language":"ja","@value":"植物における硝酸シグナル伝達の総合理解"},{"@language":"en","@value":"Comprehensive understanding of nitrate signaling in plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782004003840","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18K14728"},{"@type":"JGN","@value":"JP18K14728"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K14728/"}],"notation":[{"@language":"ja","@value":"植物の炭素/窒素制御による病原菌の感染様式に応じた抵抗性獲得機構の評価"},{"@language":"en","@value":"Evaluation of the mechanism of resistance acquisition by carbon/nitrogen control in plants according to the mode of infection of pathogens"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256972232704","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"18J01554"},{"@type":"JGN","@value":"JP18J01554"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18J01554/"}],"notation":[{"@language":"ja","@value":"植物栄養の吸収と利用の恒常性維持機構の解明"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050869456408902144","@type":"Article","resourceType":"学術雑誌論文(journal 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method: a new method for reconstructing phylogenetic trees."}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519425671808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519568193024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520073406208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520635183104","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Brix domain protein family – a key to the ribosomal biogenesis pathway?"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521108688000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Organization of Cytoplasmic Ribosomal Protein Genes in the Arabidopsis Genome"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993403437440","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An Agrobacterium-mediated transient gene expression system for intact leaves"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994584625536","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene 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snoRNA involved in rRNA methylation in Arabidopsis thaliana"},{"@language":"en","@value":"Fibrillarin genes encode both a conserved nucleolar protein and a novel small nucleolar RNA involved in ribosomal RNA methylation in Arabidopsis thaliana."}]},{"@id":"https://cir.nii.ac.jp/crid/1364233271035677952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An Arabidopsis divergent pumilio protein, <scp>APUM</scp>24, is essential for embryogenesis and required for faithful pre‐<scp>rRNA</scp> processing"}]},{"@id":"https://cir.nii.ac.jp/crid/1390850412754124032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Ribosome biogenesis factor OLI2 and its interactor BRX1-2 are associated with morphogenesis and lifespan extension in <i>Arabidopsis thaliana</i>"},{"@value":"Ribosome biogenesis factor OLI2 and its interactor BRX1-2 are associated with morphogenesis and lifespan extension in Arabidopsis thaliana"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.3389/fpls.2018.01177"},{"@type":"KAKEN","@value":"PRODUCT-22170203"},{"@type":"KAKEN","@value":"PRODUCT-22271345"},{"@type":"KAKEN","@value":"PRODUCT-23148040"},{"@type":"OPENAIRE","@value":"doi_dedup___::1ac803b0e573ded0e362491ba62bb6a2"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.20.1224a_references_DOI_9LhsiPaFtKg8ZKSNg8RKwGWzgZ6"}]}