{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004239692894208.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3892/ol.2015.4011"}},{"identifier":{"@type":"URI","@value":"https://spandidos-publications.com/10.3892/ol.2015.4011/download"}},{"identifier":{"@type":"PMID","@value":"26893686"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Regulation and clinical significance of the hypoxia-induced expression of ANGPTL4 in gastric cancer"}],"description":[{"notation":[{"@value":"Solid tumors are often exposed to hypoxia. Hypoxia inducible factor (HIF)-1α upregulates numerous target genes associated with the malignant behavior of hypoxic cancer cells. A member of the angiopoietin family, angiopoietin-like protein 4 (ANGPTL4) is a hypoxia-inducible gene. The present study aimed to clarify whether ANGPTL4 is regulated by HIF-1α in gastric cancer cells. The study also assessed whether ANGPTL4 expression is associated with clinicopathological factors or HIF-1α expression in gastric cancer tissues. Hypoxia-induced ANGPTL4 expression was quantitatively analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in 10 gastric cancer cell lines. RT-qPCR was further employed to investigate the HIF-1α dependency of ANGPTL4 expression using HIF-1α-knockdown transfectant 58As9-KD and control 58As9-SC gastric cancer cells. The HIF-1α and ANGPTL4 expression levels were immunohistochemically analyzed in 170 gastric cancer tissue specimens and were assessed for any correlations with the clinicopathological factors and/or patient survival. Subsequently, hypoxia-induced ANGPTL4 expression was observed in 7 out of 10 gastric cancer cell lines. The hypoxic induction of ANGPTL4 was almost preserved in the 58As9-KD cells compared with that observed in the 58As9-SC cells, while the induction of known HIF-1α target gene, carbonic anhydrase 9, was completely suppressed in the 58As9-KD cells. In the gastric cancer tissues, ANGPTL4 expression was inversely correlated with the tumor depth, whereas HIF-1α expression was positively correlated with venous invasion. A survival analysis revealed that the expression of ANGPTL4 was significantly correlated with a longer survival time, whereas that of HIF-1α was correlated with a shorter survival time. In conclusion, the present findings indicate that hypoxia-induced ANGPTL4 expression is independent of HIF-1α in hypoxic gastric cancer cells. ANGPTL4 may be a favorable marker for predicting a long survival time, whereas HIF-1α predicts a poor prognosis, in gastric cancer patients. The hypoxic environment independently induces ANGPTL4 and HIF-1α, which are believed to exhibit adverse effects on tumor progression."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004239692894208","@type":"Researcher","foaf:name":[{"@value":"HIROSHI KUBO"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894213","@type":"Researcher","foaf:name":[{"@value":"YOSHIHIKO KITAJIMA"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326211846656","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60516540"},{"@type":"NRID","@value":"1000060516540"},{"@type":"NRID","@value":"9000362198772"},{"@type":"NRID","@value":"9000391663608"},{"@type":"NRID","@value":"9000403282809"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/kai-saga"}],"foaf:name":[{"@value":"KEITA KAI"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894210","@type":"Researcher","foaf:name":[{"@value":"JUN NAKAMURA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894212","@type":"Researcher","foaf:name":[{"@value":"SHUUSUKE MIYAKE"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894214","@type":"Researcher","foaf:name":[{"@value":"KAZUYOSHI YANAGIHARA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894217","@type":"Researcher","foaf:name":[{"@value":"KIYOTO MORITO"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894209","@type":"Researcher","foaf:name":[{"@value":"TOMOKAZU TANAKA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894211","@type":"Researcher","foaf:name":[{"@value":"MASAAKI SHIDA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239692894216","@type":"Researcher","foaf:name":[{"@value":"HIROKAZU NOSHIRO"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"17921074"},{"@type":"EISSN","@value":"17921082"}],"prism:publicationName":[{"@value":"Oncology Letters"}],"dc:publisher":[{"@value":"Spandidos 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