{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360004239694731776.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3892/or.2017.5894"}},{"identifier":{"@type":"PMID","@value":"28849209"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Chemotherapy enhances programmed cell death 1/ligand 1 expression via TGF-β induced epithelial mesenchymal transition in non-small cell lung cancer"}],"description":[{"notation":[{"@value":"In cancer immunology, the programmed cell death 1-programmed cell death 1/ligand 1 (PD-1/PD-L1) pathway plays a major role. Anti-PD-1 and anti-PD-L1 antibodies provide reliable immunotherapy when given as treatment for various types of malignancy including lung cancer. PD-L1 expression in cancer cells has been reported to be a predictive factor for the therapeutic effects of immunotherapy. However, the mechanism of PD-L1 expression remains unclear. Another key process in cancer progression is epithelial-mesenchymal transition (EMT). In the present study, we investigated the mechanism of PD-L1 expression as well as changes in its expression during the EMT process in non-small cell lung cancer (NSCLC). In this study, A549 cells underwent EMT by treatment with TGF-β or chemotherapeutic agents and then PD-L1 expression was evaluated. The alterations of PD-L1 expression was also examined during the reverse EMT process; mesenchymal-epithelial transition (MET). The relationship between for PD-L1 expression and EMT status in clinical specimens with NSCLC after induction chemotherapy were analyzed by immunohistochemical staining. We found that PD-L1 expression was upregulated following TGF-β induction; in contrast, it was downregulated by TGF-β receptor-kinase inhibitors and the MET process. Furthermore, chemo-treatment increased TGF-β expression and enhances PD-L1 expression via autocrine TGF-β induced EMT. Analysis of clinical samples revealed a significant relationship between PD-L1 expression and EMT status (P0.05). In conclusion, our results suggest that PD-L1 expression is regulated by TGF-β induced EMT and enhanced by chemo-treatment via the chemo-induced TGF-β signaling. The anti-PD-1/PD-L1 blockade may provide more effective anticancer activities in combination with chemotherapy in NSCLC."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420001326232901760","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50464251"},{"@type":"NRID","@value":"1000050464251"},{"@type":"KAKEN_RESEARCHERS","@value":"50465241"},{"@type":"NRID","@value":"1000050465241"},{"@type":"NRID","@value":"9000302390751"},{"@type":"NRID","@value":"9000412568169"},{"@type":"NRID","@value":"9000391587184"},{"@type":"NRID","@value":"9000258011688"},{"@type":"NRID","@value":"9000406388867"},{"@type":"NRID","@value":"9000411008553"},{"@type":"NRID","@value":"9000020685639"},{"@type":"NRID","@value":"9000243899762"},{"@type":"NRID","@value":"9000411477307"},{"@type":"NRID","@value":"9000414927494"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/so1"}],"foaf:name":[{"@value":"Soichiro 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Shintani"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239694731780","@type":"Researcher","foaf:name":[{"@value":"Tomohiro Kawamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326206622720","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10779060"},{"@type":"NRID","@value":"1000010779060"}],"foaf:name":[{"@value":"Ryu Kanzaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239694731779","@type":"Researcher","foaf:name":[{"@value":"Masato Minami"}]},{"@id":"https://cir.nii.ac.jp/crid/1380004239694731776","@type":"Researcher","foaf:name":[{"@value":"Meinoshin Okumura"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"1021335X"},{"@type":"EISSN","@value":"17912431"}],"prism:publicationName":[{"@value":"Oncology Reports"}],"dc:publisher":[{"@value":"Spandidos Publications"}],"prism:publicationDate":"2017-04","prism:volume":"38","prism:number":"4","prism:startingPage":"2277","prism:endingPage":"2284"},"reviewed":"false","createdAt":"2017-08-09","modifiedAt":"2020-10-14","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Epithelial-Mesenchymal%20Transition","dc:title":"Epithelial-Mesenchymal Transition"},{"@id":"https://cir.nii.ac.jp/all?q=Programmed%20Cell%20Death%201%20Receptor","dc:title":"Programmed Cell Death 1 Receptor"},{"@id":"https://cir.nii.ac.jp/all?q=Receptor,%20Transforming%20Growth%20Factor-beta%20Type%20II","dc:title":"Receptor, Transforming Growth Factor-beta Type II"},{"@id":"https://cir.nii.ac.jp/all?q=Antineoplastic%20Agents","dc:title":"Antineoplastic Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Middle%20Aged","dc:title":"Middle Aged"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Serine-Threonine%20Kinases","dc:title":"Protein Serine-Threonine Kinases"},{"@id":"https://cir.nii.ac.jp/all?q=B7-H1%20Antigen","dc:title":"B7-H1 Antigen"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Neoplastic","dc:title":"Gene Expression Regulation, Neoplastic"},{"@id":"https://cir.nii.ac.jp/all?q=A549%20Cells","dc:title":"A549 Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Transforming%20Growth%20Factor%20beta","dc:title":"Transforming Growth Factor beta"},{"@id":"https://cir.nii.ac.jp/all?q=Carcinoma,%20Non-Small-Cell%20Lung","dc:title":"Carcinoma, Non-Small-Cell Lung"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Kinase%20Inhibitors","dc:title":"Protein Kinase Inhibitors"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Transforming%20Growth%20Factor%20beta","dc:title":"Receptors, Transforming Growth Factor beta"},{"@id":"https://cir.nii.ac.jp/all?q=Signal%20Transduction","dc:title":"Signal 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