{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360009142867439488.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1371/journal.pntd.0008844"}},{"identifier":{"@type":"URI","@value":"https://dx.plos.org/10.1371/journal.pntd.0008844"}},{"identifier":{"@type":"PMID","@value":"33151941"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Implementation of lateral flow devices (LFDs) for rabies antigen detection is expected to improve surveillance through the efficient detection of rabid animals in resource-limited settings; however, the use of LFDs for diagnosis remains controversial because some commercially available kits show low sensitivity. Therefore, we compared the diagnostic efficacy of three LFDs (ADTEC, Bionote, and Elabscience kits) paralleled with the direct fluorescent antibody test (dFAT) using fresh samples and investigated the diagnostic accuracies. To do so, we evaluated rabies-suspected samples submitted to the Regional Animal Disease Diagnostic Laboratory III, Philippines. Furthermore, we conducted real-time RT-PCR and sequencing to measure the accuracy of field laboratory diagnosis. The total number of animals submitted during this study period was 184 cases, including negative control samples. Of these, 53.9% (84 cases) were positive in the dFAT. Dogs were the most common rabies-suspected animal (n = 135). The sensitivities of the ADTEC and Bionote kits were 0.88 (74 cases) and 0.95 (80 cases), respectively. The specificity of both kits was 1.00 (100 cases). Furthermore, the sensitivity and specificity of the ADTEC kit after directly homogenizing the samples in assay buffer without dilution in phosphate-buffered saline (ADTEC kit DM) were 0.94 (79 cases) and 1.00 (100 cases), respectively. By contrast, there were no positive results using the Elabscience kit among all dFAT-positive samples. The sensitivity and specificity of LFDs make these tests highly feasible if properly used. Therefore, LFD tests can be used to strengthen the surveillance of rabies-infected animals in endemic and resource-limited settings.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801183410560","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10829724"},{"@type":"NRID","@value":"1000010829724"}],"foaf:name":[{"@value":"Kazunori Kimitsuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439375","@type":"Researcher","foaf:name":[{"@value":"Nobuo Saito"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439235","@type":"Researcher","foaf:name":[{"@value":"Kentaro Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439496","@type":"Researcher","foaf:name":[{"@value":"Chun-Ho Park"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439492","@type":"Researcher","foaf:name":[{"@value":"Satoshi Inoue"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439368","@type":"Researcher","foaf:name":[{"@value":"Motoi Suzuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439505","@type":"Researcher","foaf:name":[{"@value":"Mariko Saito-Obata"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439379","@type":"Researcher","foaf:name":[{"@value":"Yasuhiko Kamiya"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439381","@type":"Researcher","foaf:name":[{"@value":"Daria L. Manalo"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439384","@type":"Researcher","foaf:name":[{"@value":"Catalino S. Demetria"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439370","@type":"Researcher","foaf:name":[{"@value":"Milagros R. Mananggit"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439363","@type":"Researcher","foaf:name":[{"@value":"Beatriz P. Quiambao"}]},{"@id":"https://cir.nii.ac.jp/crid/1380009142867439233","@type":"Researcher","foaf:name":[{"@value":"Akira Nishizono"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1380009142867439488","@type":"Researcher","foaf:name":[{"@value":"Jesse Blanton"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"19352735"}],"prism:publicationName":[{"@value":"PLOS Neglected Tropical Diseases"}],"dc:publisher":[{"@value":"Public Library of Science (PLoS)"}],"prism:publicationDate":"2020-11-05","prism:volume":"14","prism:number":"11","prism:startingPage":"e0008844"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://dx.plos.org/10.1371/journal.pntd.0008844"}],"createdAt":"2020-11-05","modifiedAt":"2020-11-17","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Immunoassay","dc:title":"Immunoassay"},{"@id":"https://cir.nii.ac.jp/all?q=Rabies","dc:title":"Rabies"},{"@id":"https://cir.nii.ac.jp/all?q=RC955-962","dc:title":"RC955-962"},{"@id":"https://cir.nii.ac.jp/all?q=Real-Time%20Polymerase%20Chain%20Reaction","dc:title":"Real-Time Polymerase Chain Reaction"},{"@id":"https://cir.nii.ac.jp/all?q=Sensitivity%20and%20Specificity","dc:title":"Sensitivity and Specificity"},{"@id":"https://cir.nii.ac.jp/all?q=Dogs","dc:title":"Dogs"},{"@id":"https://cir.nii.ac.jp/all?q=Fluorescent%20Antibody%20Technique,%20Direct","dc:title":"Fluorescent Antibody Technique, Direct"},{"@id":"https://cir.nii.ac.jp/all?q=Rabies%20virus","dc:title":"Rabies virus"},{"@id":"https://cir.nii.ac.jp/all?q=Arctic%20medicine.%20Tropical%20medicine","dc:title":"Arctic medicine. Tropical medicine"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Reagent%20Kits,%20Diagnostic","dc:title":"Reagent Kits, Diagnostic"},{"@id":"https://cir.nii.ac.jp/all?q=Public%20aspects%20of%20medicine","dc:title":"Public aspects of medicine"},{"@id":"https://cir.nii.ac.jp/all?q=RA1-1270","dc:title":"RA1-1270"},{"@id":"https://cir.nii.ac.jp/all?q=Antigens,%20Viral","dc:title":"Antigens, Viral"},{"@id":"https://cir.nii.ac.jp/all?q=Research%20Article","dc:title":"Research Article"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782022671616","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19K16001"},{"@type":"JGN","@value":"JP19K16001"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K16001/"}],"notation":[{"@language":"ja","@value":"高感度生体イメージング法を用いた狂犬病ウイルス潜伏感染部位の同定と診断応用"},{"@language":"en","@value":"Identification and diagnostic application of rabies virus latent infection site 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