{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360011143577406464.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.91.9.3814"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.91.9.3814"}}],"dc:title":[{"@value":"Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Cytotoxic necrotizing factor type 2 (CNF2) produced by Escherichia coli strains isolated from intestinal and extraintestinal infections is a dermonecrotic toxin of 110 kDa. We cloned the CNF2 gene from a large plasmid carried by an Escherichia coli strain isolated from a lamb with septicemia. Hydropathy analysis of the deduced amino acid sequence revealed a largely hydrophilic protein with two potential hydrophobic transmembrane domains. The N-terminal half of CNF2 showed striking homology (27% identity and 80% conserved residues) to the N-terminal portion of Pasteurella multocida toxin. Methylamine protection experiments and immunofluorescence studies suggested that CNF2 enters the cytosol of the target cell through an acidic compartment and induces the reorganization of actin into stress fibers. Since the formation of stress fibers in eukaryotic cells involves Rho proteins, we radiolabeled these small GTP-binding proteins from CNF2-treated and control cells with a Rho-specific ADP-ribosyltransferase. The [32P]ADP-ribosylated Rho proteins from CNF2-treated cells migrated slightly more slowly in SDS/PAGE than did the labeled proteins from the control cells. This shift in mobility of Rho proteins in SDS/PAGE was also observed when CNF2 and the RhoA protein were coexpressed in E. coli. We propose that Rho proteins are the targets of CNF2 in mammalian cells.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380576120513040900","@type":"Researcher","foaf:name":[{"@value":"E Oswald"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120513040897","@type":"Researcher","foaf:name":[{"@value":"M Sugai"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120513040901","@type":"Researcher","foaf:name":[{"@value":"A Labigne"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120513040896","@type":"Researcher","foaf:name":[{"@value":"H C Wu"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120513040898","@type":"Researcher","foaf:name":[{"@value":"C Fiorentini"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120513040902","@type":"Researcher","foaf:name":[{"@value":"P Boquet"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]},{"@id":"https://cir.nii.ac.jp/crid/1380576120513040899","@type":"Researcher","foaf:name":[{"@value":"A D O'Brien"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00278424"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"1994-04-26","prism:volume":"91","prism:number":"9","prism:startingPage":"3814","prism:endingPage":"3818"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.91.9.3814"}],"createdAt":"2006-05-31","modifiedAt":"2022-04-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004233976446208","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Characterization of the Membrane-targeting C1 Domain in Pasteurella multocida Toxin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710699597440","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enzymatic actions of <i>Pasteurella multocida</i> toxin detected by monoclonal antibodies recognizing the deamidated α subunit of the heterotrimeric GTPase G<sub>q</sub>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576966732694016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Swine Atrophic Rhinitis Caused by Pasteurella multocida Toxin and Bordetella Dermonecrotic Toxin"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681402496128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Pathogenicity of Escherichia coli from Dogs with UTI in Relation  to Urovirulence Factors."},{"@language":"ja","@value":"内科学:犬の尿路感染症由来大腸菌の病原性発現における尿路病原因子の関連性(短報)"},{"@value":"Pathogenicity of Eschericia coli from dogs with UTI in relation to urovirulence factors"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.91.9.3814"},{"@type":"CROSSREF","@value":"10.1074/jbc.m110.102285_references_DOI_76icZ9UXmIJf039P0nX3Nor20q1"},{"@type":"CROSSREF","@value":"10.1111/j.1742-4658.2011.08197.x_references_DOI_76icZ9UXmIJf039P0nX3Nor20q1"},{"@type":"CROSSREF","@value":"10.1292/jvms.62.1197_references_DOI_76icZ9UXmIJf039P0nX3Nor20q1"},{"@type":"CROSSREF","@value":"10.1007/82_2012_206_references_DOI_76icZ9UXmIJf039P0nX3Nor20q1"}]}