{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360011144091543680.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aem.00062-14"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AEM.00062-14"}}],"dc:title":[{"@value":"Identification of Bacteriophages for Biocontrol of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. actinidiae"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Pseudomonas syringae</jats:named-content>\n            pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (\n            <jats:named-content content-type=\"genus-species\">Actinidia</jats:named-content>\n            sp.). Since 2008, a global outbreak of\n            <jats:named-content content-type=\"genus-species\">P. syringae</jats:named-content>\n            pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in\n            <jats:named-content content-type=\"genus-species\">P. syringae</jats:named-content>\n            pv. actinidiae and other pathovars against antibiotics and copper sprays have led to a search for alternative management strategies. We isolated 275 phages, 258 of which were active against\n            <jats:named-content content-type=\"genus-species\">P. syringae</jats:named-content>\n            pv. actinidiae. Extensive host range testing on\n            <jats:named-content content-type=\"genus-species\">P. syringae</jats:named-content>\n            pv. actinidiae, other pseudomonads, and bacteria isolated from kiwifruit orchards showed that most phages have a narrow host range. Twenty-four were analyzed by electron microscopy, pulse-field gel electrophoresis, and restriction digestion. Their suitability for biocontrol was tested by assessing stability and the absence of lysogeny and transduction. A detailed host range was performed, phage-resistant bacteria were isolated, and resistance to other phages was examined. The phages belonged to the\n            <jats:named-content content-type=\"genus-species\">Caudovirales</jats:named-content>\n            and were analyzed based on morphology and genome size, which showed them to be representatives of\n            <jats:named-content content-type=\"genus-species\">Myoviridae</jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Podoviridae</jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Siphoviridae</jats:named-content>\n            . Twenty-one\n            <jats:named-content content-type=\"genus-species\">Myoviridae</jats:named-content>\n            members have similar morphologies and genome sizes yet differ in restriction patterns, host range, and resistance, indicating a closely related group. Nine of these\n            <jats:named-content content-type=\"genus-species\">Myoviridae</jats:named-content>\n            members were sequenced, and each was unique. The most closely related sequenced phages were a group infecting\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa</jats:named-content>\n            and characterized by phages JG004 and PAK_P1. In summary, this study reports the isolation and characterization of\n            <jats:named-content content-type=\"genus-species\">P. syringae</jats:named-content>\n            pv. actinidiae phages and provides a framework for the intelligent formulation of phage biocontrol agents against kiwifruit bacterial canker.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380011144091543686","@type":"Researcher","foaf:name":[{"@value":"Rebekah A. Frampton"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144091543683","@type":"Researcher","foaf:name":[{"@value":"Corinda Taylor"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144091543687","@type":"Researcher","foaf:name":[{"@value":"Angela V. Holguín Moreno"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144091543680","@type":"Researcher","foaf:name":[{"@value":"Sandra B. Visnovsky"}],"jpcoar:affiliationName":[{"@value":"New Zealand Institute for Plant and Food Research Limited, Christchurch, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144091543685","@type":"Researcher","foaf:name":[{"@value":"Nicola K. Petty"}],"jpcoar:affiliationName":[{"@value":"The ithree institute, University of Technology Sydney, Sydney, Australia"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144091543681","@type":"Researcher","foaf:name":[{"@value":"Andrew R. Pitman"}],"jpcoar:affiliationName":[{"@value":"New Zealand Institute for Plant and Food Research Limited, Christchurch, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144091543684","@type":"Researcher","foaf:name":[{"@value":"Peter C. Fineran"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1380011144091543682","@type":"Researcher","foaf:name":[{"@value":"H. Goodrich-Blair"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00992240"},{"@type":"EISSN","@value":"10985336"}],"prism:publicationName":[{"@value":"Applied and Environmental Microbiology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2014-04","prism:volume":"80","prism:number":"7","prism:startingPage":"2216","prism:endingPage":"2228"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AEM.00062-14"}],"createdAt":"2014-02-01","modifiedAt":"2022-02-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050290932560855936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Isolation and characterization of a novel jumbo phage from leaf litter compost and its suppressive effect on rice seedling rot diseases."}]},{"@id":"https://cir.nii.ac.jp/crid/1360580229791504000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Isolation of Burkholderia jumbo phages and their utilization as biocontrol agents to suppress rice seedling rot disease"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/aem.00062-14"},{"@type":"CROSSREF","@value":"10.3390/v13040591_references_DOI_ZGnY9J4CjeLDo8EpWO0Rhu52yJC"},{"@type":"CROSSREF","@value":"10.1007/s10327-022-01107-z_references_DOI_ZGnY9J4CjeLDo8EpWO0Rhu52yJC"}]}