{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360011144152134656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1006/abbi.1995.1094"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0003986185710946?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0003986185710946?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"7864625"}}],"dc:title":[{"@value":"Characterization of an Inducible P450 Hydroxylase Involved in the Rice Diterpene Phytoalexin Biosynthetic Pathway"}],"description":[{"notation":[{"@value":"Ent-isopimara-8(14),15-dien-3 beta-ol (1) was isolated from uv-irradiated rice (Oryza sativa L.) leaves. Since 1 was converted to the rice diterpene phytoalexins and oryzalexins D (ent-isopimara-8(14),15-diene-3 beta,7 alpha-diol) and E (ent-isopimara-8(14),15-diene-3 beta,9 alpha-diol) in uv-irradiated rice leaf microsome fraction in the presence of oxygen and NADPH, it was concluded that 1 was the biosynthetic precursor of these oryzalexins. This enzyme reaction was inhibited by cytochrome P450 inhibitors such as piperonyl butoxide, SKF-525A, paclobutorazole, metyrapone, cytochrome c, and carbon monoxide. The lack of inhibitory activity of 1 for spore germination of Pyricularia oryzae indicates that the final cytochrome P450-dependent hydroxylation steps in converting 1 to oryzalexins D and E are essential for the production of rice phytoalexins with antifungal activities."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380011144152134656","@type":"Researcher","foaf:name":[{"@value":"H. Kato"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144152134657","@type":"Researcher","foaf:name":[{"@value":"O. Kodama"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144152134658","@type":"Researcher","foaf:name":[{"@value":"T. Akatsuka"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00039861"}],"prism:publicationName":[{"@value":"Archives of Biochemistry and Biophysics"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"1995-02","prism:volume":"316","prism:number":"2","prism:startingPage":"707","prism:endingPage":"712"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0003986185710946?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0003986185710946?httpAccept=text/plain"}],"createdAt":"2002-10-07","modifiedAt":"2019-05-07","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Antifungal%20Agents","dc:title":"Antifungal Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Extracts","dc:title":"Plant Extracts"},{"@id":"https://cir.nii.ac.jp/all?q=Terpenes","dc:title":"Terpenes"},{"@id":"https://cir.nii.ac.jp/all?q=Ultraviolet%20Rays","dc:title":"Ultraviolet Rays"},{"@id":"https://cir.nii.ac.jp/all?q=Oryza","dc:title":"Oryza"},{"@id":"https://cir.nii.ac.jp/all?q=Spores,%20Fungal","dc:title":"Spores, Fungal"},{"@id":"https://cir.nii.ac.jp/all?q=Mixed%20Function%20Oxygenases","dc:title":"Mixed Function Oxygenases"},{"@id":"https://cir.nii.ac.jp/all?q=Phytoalexins","dc:title":"Phytoalexins"},{"@id":"https://cir.nii.ac.jp/all?q=Cytochrome%20P-450%20Enzyme%20Inhibitors","dc:title":"Cytochrome P-450 Enzyme Inhibitors"},{"@id":"https://cir.nii.ac.jp/all?q=Diterpenes","dc:title":"Diterpenes"},{"@id":"https://cir.nii.ac.jp/all?q=Sesquiterpenes","dc:title":"Sesquiterpenes"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282679176773504","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"イネのファイトアレキシン生合成遺伝子クラスター　　その発見と協調的な発現制御メカニズム"},{"@language":"en","@value":"Biosynthetic Gene Clusters for Phytoalexin in Rice"},{"@language":"ja-Kana","@value":"イネ ノ ファイトアレキシン セイゴウセイ イデンシ クラスター ソノ ハッケン ト キョウチョウテキナ ハツゲン セイギョ メカニズム"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303316992","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Transcriptional regulation of the biosynthesis of phytoalexin: A lesson from specialized metabolites in rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681456513792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Biosynthesis of Phytoalexins and Regulatory Mechanisms of It in Rice"},{"@value":"Award Review : Biosynthesis of Phytoalexins and Regulatory Mechanisms of It in Rice"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1006/abbi.1995.1094"},{"@type":"OPENAIRE","@value":"doi_dedup___::1f6bfc8f28fa5efd3fa00addbad795b8"},{"@type":"CROSSREF","@value":"10.1271/bbb.130109_references_DOI_SOKuF5mfDIT7itGPCMZyweHQ890"},{"@type":"CROSSREF","@value":"10.1271/kagakutoseibutsu.47.43_references_DOI_SOKuF5mfDIT7itGPCMZyweHQ890"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.0730a_references_DOI_SOKuF5mfDIT7itGPCMZyweHQ890"}]}