{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360011144781908352.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/sj.onc.1208716"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/1208716.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/1208716"}},{"identifier":{"@type":"PMID","@value":"16007216"}}],"dc:title":[{"@value":"The Brm gene suppressed at the post-transcriptional level in various human cell lines is inducible by transient HDAC inhibitor treatment, which exhibits antioncogenic potential"}],"description":[{"notation":[{"@value":"The mammalian SWI/SNF chromatin remodeling complex is composed of more than 10 protein subunits, and plays important roles in epigenetic regulation. Each complex includes a single BRG1 or Brm molecule as the catalytic subunit. We previously reported that loss of Brm, but not BRG1, causes transcriptional gene silencing of murine leukemia virus-based retrovirus vectors. To understand the biological function and biogenesis of Brm protein, we examined seven cell lines derived from various human tumors that do not produce Brm protein. We show here that these Brm-deficient cell lines transcribe the Brm genes efficiently as detected by nuclear run-on transcription assay, whereas Brm mRNA and Brm hnRNA were undetectable by reverse transcription-polymerase chain reaction analysis. These results indicate that expression of Brm is strongly and promptly suppressed at the post-transcriptional level, through processing and transport of the primary transcript or through stability of mature Brm mRNA. This suppression was attenuated by transient treatment of these cell lines with HDAC inhibitors probably through indirect mechanism. Importantly, all of the treated cells showed prolonged induction of Brm expression after the removal of HDAC inhibitors, and acquired the ability to maintain retroviral gene expression. These results indicate that these Brm-deficient human tumor cell lines carry a functional Brm gene. Treatment with HDAC inhibitors or introduction of exogenous Brm into Brm-deficient cell lines significantly reduced the oncogenic potential as assessed by colony-forming activity in soft agar or invasion into collagen gel, indicating that, like BRG1, Brm is involved in tumor suppression."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380011144781908356","@type":"Researcher","foaf:name":[{"@value":"Nobutake Yamamichi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908484","@type":"Researcher","foaf:name":[{"@value":"Mitsue Yamamichi-Nishina"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908353","@type":"Researcher","foaf:name":[{"@value":"Taketoshi Mizutani"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908482","@type":"Researcher","foaf:name":[{"@value":"Hirotaka Watanabe"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908357","@type":"Researcher","foaf:name":[{"@value":"Shigeru Minoguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908481","@type":"Researcher","foaf:name":[{"@value":"Nao Kobayashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908354","@type":"Researcher","foaf:name":[{"@value":"Satoko Kimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908352","@type":"Researcher","foaf:name":[{"@value":"Taiji Ito"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908483","@type":"Researcher","foaf:name":[{"@value":"Naohisa Yahagi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908358","@type":"Researcher","foaf:name":[{"@value":"Masao Ichinose"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011144781908480","@type":"Researcher","foaf:name":[{"@value":"Masao Omata"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658301054727","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60111449"},{"@type":"NRID","@value":"1000060111449"},{"@type":"NRID","@value":"9000016809516"},{"@type":"NRID","@value":"9000020555674"},{"@type":"NRID","@value":"9000253469944"},{"@type":"NRID","@value":"9000001118020"},{"@type":"NRID","@value":"9000016912096"},{"@type":"NRID","@value":"9000007167261"},{"@type":"NRID","@value":"9000009537615"},{"@type":"NRID","@value":"9000253200866"},{"@type":"NRID","@value":"9000255783716"},{"@type":"NRID","@value":"9000402486184"},{"@type":"NRID","@value":"9000391625682"},{"@type":"NRID","@value":"9000010743701"},{"@type":"NRID","@value":"9000017149649"},{"@type":"NRID","@value":"9000254056612"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/iba"}],"foaf:name":[{"@value":"Hideo Iba"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09509232"},{"@type":"EISSN","@value":"14765594"}],"prism:publicationName":[{"@value":"Oncogene"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2005-06-20","prism:volume":"24","prism:number":"35","prism:startingPage":"5471","prism:endingPage":"5481"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.springer.com/tdm","https://www.springer.com/tdm"],"url":[{"@id":"https://www.nature.com/articles/1208716.pdf"},{"@id":"https://www.nature.com/articles/1208716"}],"createdAt":"2005-07-12","modifiedAt":"2022-03-22","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Transcription,%20Genetic","dc:title":"Transcription, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Chromosomal%20Proteins,%20Non-Histone","dc:title":"Chromosomal Proteins, Non-Histone"},{"@id":"https://cir.nii.ac.jp/all?q=Reverse%20Transcriptase%20Polymerase%20Chain%20Reaction","dc:title":"Reverse Transcriptase Polymerase Chain Reaction"},{"@id":"https://cir.nii.ac.jp/all?q=Blotting,%20Western","dc:title":"Blotting, Western"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Helicases","dc:title":"DNA Helicases"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression","dc:title":"Gene Expression"},{"@id":"https://cir.nii.ac.jp/all?q=Nuclear%20Proteins","dc:title":"Nuclear Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=SMARCB1%20Protein","dc:title":"SMARCB1 Protein"},{"@id":"https://cir.nii.ac.jp/all?q=DNA-Binding%20Proteins","dc:title":"DNA-Binding Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Histone%20Deacetylase%20Inhibitors","dc:title":"Histone Deacetylase Inhibitors"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Transformation,%20Neoplastic","dc:title":"Cell Transformation, Neoplastic"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation","dc:title":"Gene Expression Regulation"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=RNA%20Interference","dc:title":"RNA Interference"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Messenger","dc:title":"RNA, Messenger"},{"@id":"https://cir.nii.ac.jp/all?q=Enzyme%20Inhibitors","dc:title":"Enzyme Inhibitors"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription%20Factors","dc:title":"Transcription Factors"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002218689886080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"SNF5/INI1 Deficiency Redefines Chromatin Remodeling Complex Composition during Tumor Development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233478977024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"MiR-199a Inhibits Secondary Envelopment of Herpes Simplex Virus-1 Through the Downregulation of Cdc42-specific GTPase Activating Protein Localized in Golgi Apparatus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233528402688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004237516463360","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cathepsin E Is a Marker of Gastric Differentiation and Signet-Ring Cell Carcinoma of Stomach: A Novel Suggestion on Gastric Tumorigenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706977228800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rebamipide induces dendritic cell recruitment to N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-exposed rat gastric mucosa based on IL-1β upregulation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708453633280","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The role of the SWI/SNF chromatin remodeling complex in maintaining the stemness of glioma initiating cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710467141248","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transduced caudal‐type homeobox (<scp>CDX</scp>) 2/<scp>CDX</scp>1 can induce growth inhibition on <scp>CDX</scp>‐deficient gastric cancer by rapid intestinal differentiation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710962508928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Phosphorylation of Herpes Simplex Virus 1 dUTPase Upregulated Viral dUTPase Activity To Compensate for Low Cellular dUTPase Activity for Efficient Viral Replication"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285712530300160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Gli Regulates MUC5AC Transcription in Human Gastrointestinal Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567180041777152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Use of cell type‐specific transcriptome to identify genes specifically involved in Müller glia differentiation during retinal development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183431338496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Analysis of Müller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse"}]},{"@id":"https://cir.nii.ac.jp/crid/1360572092648340480","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"BRG1, INI1, and ARID1B Deficiency in Endometrial Carcinoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580232163331968","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transcriptome analysis reveals the essential role of NK2 homeobox 1/thyroid transcription factor 1 (NKX2-1/TTF-1) in gastric adenocarcinoma of fundic-gland type"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848655018515456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"BMP signaling participates in late phase differentiation of the retina, partly via upregulation of <i>Hey2</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660482669312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Serine/threonine kinase, Melk, regulates proliferation and glial differentiation of retinal progenitor cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1038/sj.onc.1208716"},{"@type":"OPENAIRE","@value":"doi_dedup___::ff92f06489b57175de8541f930d5bd1b"},{"@type":"CROSSREF","@value":"10.1038/s41598-017-06754-3_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1038/srep08428_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0056766_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1038/s41598-017-00982-3_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1111/cas.13821_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1128/jvi.00603-14_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0106106_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1016/j.bbrc.2012.06.087_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1002/dneu.22131_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1038/s41598-017-03874-8_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1097/pas.0000000000001581_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1007/s10120-022-01334-5_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1002/dneu.22196_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1111/j.1349-7006.2011.02104.x_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"},{"@type":"CROSSREF","@value":"10.1158/1541-7786.mcr-14-0005_references_DOI_BESl3lNGL6kyww4cmTDPPrdRYbS"}]}