{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360011146428279808.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aem.07679-11"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AEM.07679-11"}}],"dc:title":[{"@value":"Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Arabinoxylan is a heteropolymeric chain of a β-1,4-linked xylose backbone substituted with arabinose residues, representing a principal component of plant cell walls. Here we developed recombinant\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae</jats:named-content>\n            strains as whole-cell biocatalysts capable of combining hemicellulase production, xylan hydrolysis, and hydrolysate fermentation into a single step. These strains displayed a series of uni-, bi-, and trifunctional minihemicellulosomes that consisted of a miniscaffoldin (CipA3/CipA1) and up to three chimeric enzymes. The miniscaffoldin derived from\n            <jats:named-content content-type=\"genus-species\">Clostridium thermocellum</jats:named-content>\n            contained one or three cohesin modules and was tethered to the cell surface through the\n            <jats:named-content content-type=\"genus-species\">S. cerevisia</jats:named-content>\n            e a-agglutinin adhesion receptor. Up to three types of hemicellulases, an endoxylanase (XynII), an arabinofuranosidase (AbfB), and a β-xylosidase (XlnD), each bearing a C-terminal dockerin, were assembled onto the miniscaffoldin by high-affinity cohesin-dockerin interactions. Compared to uni- and bifunctional minihemicellulosomes, the resulting quaternary trifunctional complexes exhibited an enhanced rate of hydrolysis of arabinoxylan. Furthermore, with an integrated\n            <jats:sc>d</jats:sc>\n            -xylose-utilizing pathway, the recombinant yeast displaying the bifunctional minihemicellulosome CipA3-XynII-XlnD could simultaneously hydrolyze and ferment birchwood xylan to ethanol with a yield of 0.31 g per g of sugar consumed.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380011146428279808","@type":"Researcher","foaf:name":[{"@value":"Jie Sun"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA"},{"@value":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011146428279812","@type":"Researcher","foaf:name":[{"@value":"Fei Wen"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011146428279809","@type":"Researcher","foaf:name":[{"@value":"Tong Si"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011146428279810","@type":"Researcher","foaf:name":[{"@value":"Jian-He Xu"}],"jpcoar:affiliationName":[{"@value":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China"}]},{"@id":"https://cir.nii.ac.jp/crid/1380011146428279811","@type":"Researcher","foaf:name":[{"@value":"Huimin Zhao"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA"},{"@value":"Departments of Chemistry, Biochemistry, and Bioengineering, Institute for Genomic Biology, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00992240"},{"@type":"EISSN","@value":"10985336"}],"prism:publicationName":[{"@value":"Applied and Environmental Microbiology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2012-06","prism:volume":"78","prism:number":"11","prism:startingPage":"3837","prism:endingPage":"3845"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AEM.07679-11"}],"createdAt":"2012-03-24","modifiedAt":"2022-02-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360567186804491264","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biomolecular assembly strategies to develop potential artificial cellulosomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118768342912","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus Phlebia acerina"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/aem.07679-11"},{"@type":"CROSSREF","@value":"10.1186/s40508-014-0019-9_references_DOI_JtT8v1eddc8XDMKNEb0UrqV8SSD"},{"@type":"CROSSREF","@value":"10.3390/fermentation7030116_references_DOI_JtT8v1eddc8XDMKNEb0UrqV8SSD"}]}