{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360017282468340736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3390/cells11111754"}},{"identifier":{"@type":"URI","@value":"https://www.mdpi.com/2073-4409/11/11/1754/pdf"}},{"identifier":{"@type":"PMID","@value":"35681449"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Efficient Isolation of Lymphocytes and Myogenic Cells from the Tissue of Muscle Regeneration"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Isolation of both lymphocytes and myogenic cells from muscle tissue is required for elucidating the cellular and molecular mechanisms of muscle regeneration. Here, we aimed to establish an optimal method obtaining a high yield of lymphocytes during muscle regeneration. After the muscle injury, we observed higher infiltration of lymphocytic cells in the muscle on day 3 after injury. Then, we compared two different white blood cell isolation methods, the Percoll gradient and CD45-magnetic bead methods, to assess the percentage and number of T and B cells. Flow cytometry analysis showed that the CD45-magnetic bead method has a better efficiency in isolating CD4+, CD8+ T cells, and B cells from injured muscle tissues of wild-type and mdx mice than that by the Percoll gradient method. Moreover, we found that the CD45-negative fraction from wild-type and mdx mice includes myogenic cells. In conclusion, we report that the CD45-magnetic bead method is suitable to isolate T and B cells during muscle regeneration with higher purity and yield and can also isolate myogenic cells within the same sample. This method provides a technical basis for further studies on muscle regeneration, involving lymphocytes and muscle cells, with a wide range of clinical applications.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420845751154403072","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70734416"},{"@type":"NRID","@value":"1000070734416"},{"@type":"NRID","@value":"9000345329552"},{"@type":"NRID","@value":"9000350551347"},{"@type":"NRID","@value":"9000256892661"},{"@type":"NRID","@value":"9000411818019"},{"@type":"NRID","@value":"9000018945436"},{"@type":"NRID","@value":"9000014575148"},{"@type":"NRID","@value":"9000397954648"},{"@type":"NRID","@value":"9000411817222"},{"@type":"NRID","@value":"9000243891017"},{"@type":"NRID","@value":"9000411116134"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/proteasome7"}],"foaf:name":[{"@value":"Yasuo Kitajima"}],"jpcoar:affiliationName":[{"@value":"Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380017282468340740","@type":"Researcher","foaf:name":[{"@value":"Ryoka Tsukahara"}],"jpcoar:affiliationName":[{"@value":"Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380017282468340741","@type":"Researcher","foaf:name":[{"@value":"Shohei Nakamoto"}],"jpcoar:affiliationName":[{"@value":"Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326220369792","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40334429"},{"@type":"NRID","@value":"1000040334429"},{"@type":"NRID","@value":"9000002039702"},{"@type":"NRID","@value":"9000398802204"},{"@type":"NRID","@value":"9000399833400"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0099505"}],"foaf:name":[{"@value":"Tomoharu Yasuda"}],"jpcoar:affiliationName":[{"@value":"Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20734409"}],"prism:publicationName":[{"@value":"Cells"}],"dc:publisher":[{"@value":"MDPI AG"}],"prism:publicationDate":"2022-05-26","prism:volume":"11","prism:number":"11","prism:startingPage":"1754"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.mdpi.com/2073-4409/11/11/1754/pdf"}],"createdAt":"2022-05-26","modifiedAt":"2025-10-10","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=lymphocytes","dc:title":"lymphocytes"},{"@id":"https://cir.nii.ac.jp/all?q=CD45%20beads","dc:title":"CD45 beads"},{"@id":"https://cir.nii.ac.jp/all?q=muscle%20regeneration","dc:title":"muscle regeneration"},{"@id":"https://cir.nii.ac.jp/all?q=QH573-671","dc:title":"QH573-671"},{"@id":"https://cir.nii.ac.jp/all?q=Communication","dc:title":"Communication"},{"@id":"https://cir.nii.ac.jp/all?q=Muscles","dc:title":"Muscles"},{"@id":"https://cir.nii.ac.jp/all?q=CD8-Positive%20T-Lymphocytes","dc:title":"CD8-Positive T-Lymphocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Flow%20Cytometry","dc:title":"Flow Cytometry"},{"@id":"https://cir.nii.ac.jp/all?q=Muscle%20Development","dc:title":"Muscle Development"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Percoll","dc:title":"Percoll"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20mdx","dc:title":"Mice, Inbred mdx"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Cytology","dc:title":"Cytology"},{"@id":"https://cir.nii.ac.jp/all?q=myogenic%20cells","dc:title":"myogenic cells"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040285300696455040","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"20H04078"},{"@type":"JGN","@value":"JP20H04078"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H04078/"}],"notation":[{"@language":"ja","@value":"骨格筋再生における筋幹細胞の新たなダイナミクスの解明"},{"@language":"en","@value":"Elucidation of a new role of muscle stem cells in skeletal muscle regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1040287907231036672","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21H02751"},{"@type":"JGN","@value":"JP21H02751"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02751/"}],"notation":[{"@language":"ja","@value":"免疫特性と癌化に及ぼすリンパ球増殖限界のエピジェネティックな制御基盤"},{"@language":"en","@value":"Epigenetic regulation of lymphocyte cell division and oncogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1040570232414668032","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21K19720"},{"@type":"JGN","@value":"JP21K19720"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19720/"}],"notation":[{"@language":"ja","@value":"タンパク分解系に着目した新たなサルコペニア動物モデルの創出"},{"@language":"en","@value":"Examination of sarcopenic animal models focusing on the proteolytic system"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011144181489920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Single-Cell Analysis of the Muscle Stem Cell Hierarchy Identifies Heterotypic Communication Signals Involved in Skeletal Muscle Regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145672540160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Quantitative Analysis of the Contribution of TCR/pepMHC Affinity and CD8 to T Cell 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