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To solve this problem, we used glyoxal, a two-carbon atom di-aldehyde. We found that glyoxal fixation greatly improved antibody penetration and immunoreactivity, uncovering signals for buried molecules by conventional immunohistochemical procedures at light and electron microscopic levels. It also enhanced immunosignals of most other molecules, which are known to be detectable in formaldehyde-fixed sections. Furthermore, we unearthed several specific primary antibodies that were once judged to be unusable in formaldehyde-fixed tissues, allowing us to successfully localize so far controversial synaptic adhesion molecule Neuroligin 1. Thus, glyoxal is a highly effective fixative for immunostaining, and a side-by-side comparison of glyoxal and formaldehyde fixation is recommended for routine immunostaining in neuroscience research.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801206439424","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20599641"},{"@type":"NRID","@value":"1000020599641"},{"@type":"NRID","@value":"9000240124496"},{"@type":"NRID","@value":"9000409884444"},{"@type":"NRID","@value":"9000404636281"},{"@type":"NRID","@value":"9000024446165"},{"@type":"NRID","@value":"9000001686929"},{"@type":"NRID","@value":"9000018608592"},{"@type":"NRID","@value":"9000278499414"},{"@type":"NRID","@value":"9000319553687"},{"@type":"NRID","@value":"9000399769316"},{"@type":"NRID","@value":"9000237782027"},{"@type":"NRID","@value":"9000238353110"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/kotoro"}],"foaf:name":[{"@value":"Kohtarou 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