{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021390563207680.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1242/dev.202315"}},{"identifier":{"@type":"URI","@value":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.202315/3357028/dev202315.pdf"}},{"identifier":{"@type":"URI","@value":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.202315/3358985/dev202315.pdf"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Live tracking of basal stem cells of the epidermis during growth, homeostasis and injury response in zebrafish"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n               <jats:p>Basal stem cells of the epidermis continuously differentiate into keratinocytes and replenish themselves via self-renewal to maintain skin homeostasis. Numerous studies have attempted to reveal how basal cells undergo differentiation or self-renewal; however, this has been hampered by a lack of robust basal cell markers and analytical platforms that allow single-cell tracking. Here, we report that zebrafish integrin beta 4 is a useful marker for basal cell labelling, irrespective of the body region, stage and regenerative status. We employed Cre-loxP recombination in combination with live cell tracking of single basal clones in the caudal fin and investigated the embryonic origin and behaviour of basal cells during fish growth and homeostasis. Although most basal cells, including those in fins, became quiescent in the adult stage, genetic cell ablation showed that basal cells were reactivated to either self-renew or differentiate, depending on the injured cell type. Our study provides a simple and easy-to-use platform for quantitative in vivo imaging of basal stem cells at wider stages and under various conditions.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021390563207696","@type":"Researcher","foaf:name":[{"@value":"Zhengcheng Liu"}],"jpcoar:affiliationName":[{"@value":", Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390563207681","@type":"Researcher","foaf:name":[{"@value":"Yidan Meng"}],"jpcoar:affiliationName":[{"@value":", Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326226928896","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00221896"},{"@type":"NRID","@value":"1000000221896"},{"@type":"NRID","@value":"9000001905896"},{"@type":"NRID","@value":"9000018475150"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0079815"}],"foaf:name":[{"@value":"Atsushi Kawakami"}],"jpcoar:affiliationName":[{"@value":", Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390563207701","@type":"Researcher","foaf:name":[{"@value":"Ayu Ishikura"}],"jpcoar:affiliationName":[{"@value":", Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09501991"},{"@type":"EISSN","@value":"14779129"}],"prism:publicationName":[{"@value":"Development"}],"dc:publisher":[{"@value":"The Company of Biologists"}],"prism:publicationDate":"2024-01-15","prism:volume":"151","prism:number":"2"},"reviewed":"false","dc:rights":["http://www.biologists.com/user-licence-1-1/"],"url":[{"@id":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.202315/3357028/dev202315.pdf"},{"@id":"https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.202315/3358985/dev202315.pdf"}],"createdAt":"2024-01-17","modifiedAt":"2024-01-24","project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782014598912","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19H03232"},{"@type":"JGN","@value":"JP19H03232"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03232/"}],"notation":[{"@language":"ja","@value":"恒常性破綻から形態再生に至る組織再生プロセスの統合的理解を目指して"},{"@language":"en","@value":"Conprehemsive understanding of tissue regeneration processes frombreak down of homeostasis to morphological regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1040011231170896512","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22K19306"},{"@type":"JGN","@value":"JP22K19306"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19306/"}],"notation":[{"@language":"ja","@value":"組織再生における位置情報メカニズムの解明"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232095424000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145317122176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration 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Vitro"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044183635584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A single type of progenitor cell maintains normal epidermis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044926404992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Organization of Human Epidermis: Functional Epidermal Units and Phi Proportionality"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045170998784","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Proteomics of early zebrafish embryos"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518580954112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Distinct contribution of stem and progenitor cells to epidermal maintenance"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518767718400","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Lgl2 Executes Its 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